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All Journal Journal of Food and Pharmaceutical Science Jurnal Mandala Pharmacon Indonesia Journal of Food and Pharmaceutical Sciences
Yadnya-Putra, Anak Agung Gede Rai
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Aktivitas Antiinflamasi Nanoemulsi Ekstrak Kurkumin dari Kunyit (Curcuma longa L.) pada Mencit Jantan yang Di induksi Karagenan Widiantara, Komang Rian; Leticia, Dewa Ayu Putu Maylani; Wardani, Ni Nyoman Putri; Artawan, Dewa Gede Wira Putra; Dewi, Putu Claudia Sekar Sariyanti; Yadnya-Putra, Anak Agung Gede Rai
Jurnal Mandala Pharmacon Indonesia Vol. 11 No. 2 (2025): Jurnal Mandala Pharmacon Indonesia 
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v11i2.1004

Abstract

Inflamasi merupakan respon fisiologis tubuh terhadap cedera jaringan akibat trauma fisik, kimia, atau mikrobiologik. Terapi menggunakan obat antiinflamasi nonsteroid (NSAID) dan steroid sering menimbulkan efek samping serius, sehingga diperlukan alternatif berbasis bahan alam. Kunyit (Curcuma longa L.) diketahui mengandung senyawa kurkuminoid, terutama kurkumin, yang memiliki aktivitas antiinflamasi. Namun, bioavailabilitas kurkumin yang rendah membatasi efektivitasnya, sehingga dikembangkan dalam bentuk nanoemulsi untuk meningkatkan kelarutan dan penetrasi. Penelitian ini bertujuan mengetahui aktivitas antiinflamasi nanoemulsi ekstrak kunyit serta menentukan dosis optimum terhadap mencit jantan yang diinduksi karagenan 1%. Ekstraksi dilakukan menggunakan metode Ultrasound Assisted Extraction dengan pelarut etanol 96%, diikuti pembuatan nanoemulsi melalui metode emulsifikasi energi rendah menggunakan VCO, PEG 40, dan PEG 400. Nanoemulsi dievaluasi secara fisik (organoleptik, pH, viskositas, stabilitas, ukuran partikel). Rata-rata ukuran partikel sebesar 67,72 ± 1,21 nm yang berada dalam rentang ukuran nanoemulsi (20-200 nm). Uji aktivitas antiinflamasi menggunakan metode induksi karagenan menunjukkan bahwa nanoemulsi ekstrak kunyit menurunkan volume peradangan relatif secara signifikan dibandingkan kontrol negatif (Kruskal-Wallis, p = 0,009). Nilai Area Under Curve (AUC) menurun seiring peningkatan dosis, dengan hasil terbaik pada dosis 200 mg/kgBB, mendekati efektivitas kontrol positif (kalium diklofenak 6,5 mg/kgBB). Begitu pula dengan persentase hambatan peradangan yang cukup tinggi pada menit ke-300 sebesar 83,07% (200 mg/kgBB). Hasil ini menunjukkan bahwa nanoemulsi ekstrak kunyit berpotensi sebagai kandidat sediaan antiinflamasi alami yang efektif, uji lanjutan diperlukan uji toksisitas, stabilitas jangka panjang, serta uji klinis lebih lanjut guna menjamin mutu dan keamanan sediaan.
Optimization of Green Ultrasound-Assisted Extraction of Carotenoids from Tagetes erecta L. using Natural Deep Eutectic Solvents (NADES) Andriani, Ni Luh Febby; Yadnya-Putra, Anak Agung Gede Rai; Kusuma Dewi, Luh Putu Mirah
Journal of Food and Pharmaceutical Sciences Vol 14, No 2 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.22717

Abstract

Carotenoids from marigold flowers (Tagetes erecta L.) are commonly extracted using conventional solvents like hexane and ethanol, which may pose environmental and health risks. Based on these considerations, this study aimed to evaluate the potential of Natural Deep Eutectic Solvents (NADES) as greener alternatives and to determine the optimal NADES composition and extraction conditions using Ultrasound-Assisted Extraction (UAE). The most effective NADES was selected based on total carotenoid content, statistically analyzed using one-way ANOVA. Optimization was carried out using Response Surface Methodology (RSM) with a Box–Behnken Design (BBD) to assess the effects of ultrasonic power, extraction time, and temperature. Total carotenoid content was determined by UV-Vis spectrophotometry. Among the tested solvents, the hydrophobic NADES composed of menthol:lactic acid (8:1) showed the highest efficiency (98.48 ± 0.20 mg β-carotene equivalent (BCE)/g dry sample (DS)), outperforming the hydrophilic NADES, hexane, and 80% ethanol. Optimization using Response Surface Methodology with a Box–Behnken Design identified optimal UAE conditions at 100% ultrasonic power, 40 minutes, and 60°C, yielding 304.01 ± 1.60 mg BCE/g DS. These results confirm the effectiveness of NADES as sustainable and efficient solvents for carotenoid extraction from marigold flowers.
Optimization of Green Ultrasound-Assisted Extraction of Carotenoids from Tagetes erecta L. using Natural Deep Eutectic Solvents (NADES) Andriani, Ni Luh Febby; Yadnya-Putra, Anak Agung Gede Rai; Kusuma Dewi, Luh Putu Mirah
Journal of Food and Pharmaceutical Sciences Vol 14, No 2 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.22717

Abstract

Carotenoids from marigold flowers (Tagetes erecta L.) are commonly extracted using conventional solvents like hexane and ethanol, which may pose environmental and health risks. Based on these considerations, this study aimed to evaluate the potential of Natural Deep Eutectic Solvents (NADES) as greener alternatives and to determine the optimal NADES composition and extraction conditions using Ultrasound-Assisted Extraction (UAE). The most effective NADES was selected based on total carotenoid content, statistically analyzed using one-way ANOVA. Optimization was carried out using Response Surface Methodology (RSM) with a Box–Behnken Design (BBD) to assess the effects of ultrasonic power, extraction time, and temperature. Total carotenoid content was determined by UV-Vis spectrophotometry. Among the tested solvents, the hydrophobic NADES composed of menthol:lactic acid (8:1) showed the highest efficiency (98.48 ± 0.20 mg β-carotene equivalent (BCE)/g dry sample (DS)), outperforming the hydrophilic NADES, hexane, and 80% ethanol. Optimization using Response Surface Methodology with a Box–Behnken Design identified optimal UAE conditions at 100% ultrasonic power, 40 minutes, and 60°C, yielding 304.01 ± 1.60 mg BCE/g DS. These results confirm the effectiveness of NADES as sustainable and efficient solvents for carotenoid extraction from marigold flowers.
Co-Authors Andriani, Ni Luh Febby Artawan, Dewa Gede Wira Putra Dewi, Putu Claudia Sekar Sariyanti Kusuma Dewi, Luh Putu Mirah Leticia, Dewa Ayu Putu Maylani Wardani, Ni Nyoman Putri Widiantara, Komang Rian