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All Journal Journal of Tropical Animal Science and Technology Indonesian Journal of Laboratory
Rafif, Muhammad Rio
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Agriculture, Biological Sciences & Forestry Mathematics Mechanical Engineering Medicine & Pharmacology Physics

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Validation of Analysis Methods for Phosphorus in Chicken Meat and Fresh Cow's Milk Using Microwave Destruction and Spectrophotometry Microplate UV-VIS Hadianto, Ikhwan; Kusumawati, Siska Devi; Nurlatifah; Rafif, Muhammad Rio; Ispitasari, Rina
Journal of Tropical Animal Science and Technology Vol. 7 No. 3 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v7i3.10311

Abstract

Phosphorus content in animal-derived foods is an important indicator of nutritional value and food safety. This study validated a method for phosphorus analysis in chicken meat and fresh cow’s milk using wet digestion based on microwave digestion and microplate UV-Vis spectrophotometry with the principle of molybdenum blue complex formation by ascorbic acid at a wavelength of 880 nm. Method performance was evaluated according to ISO/IEC 17025:2017. The validation parameters included linearity and linear range, sensitivity comprising the limit of detection (LOD) and limit of quantification (LOQ), precision comprising repeatability and intermediate precision, as well as accuracy. The calibration curve showed good linearity within the range of 2–12 ppm (r² = 0.998). The LOD and LOQ values were 0.29 and 0.97 mg/L for chicken meat, and 0.17 and 0.57 mg/L for cow’s milk, respectively. Precision testing resulted in repeatability and intermediate precision RSDs of 2.00% and 2.18% for chicken meat, and 2.62% and 2.14% for cow’s milk. Accuracy, determined by recovery analysis, showed values of 97.14% for chicken meat and 103.15% for cow’s milk. These results demonstrate that the validated method meets the acceptance criteria of ISO/IEC 17025. The method provides acceptable linearity, sensitivity, precision, and accuracy, and can be applied for routine phosphorus analysis in meat and milk matrices. Keywords: Phosphorus, chicken meat, cow’s milk, method validation, UV-Vis spectrophotometry
Validasi Metode Analisis Kalsium pada Bungkil Kedelai dengan Metode Atomic Absorption Specthophotometry (AAS) Hadianto, Ikhwan; Kusumawati, Siska Devi; Latifah, Nur; Rafif, Muhammad Rio
Indonesian Journal of Laboratory Vol 9, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijl.v9i1.115859

Abstract

ABSTRAK. Penelitian dilakukan bertujuan untuk memvalidasi metode penentuan kadar kalsium pada bungkil kedelai menggunakan Atomic Absorption Spectrophotometry (AAS) sesuai ISO/IEC 17025:2017. Kalsium merupakan mineral essensial bagi makhluk hidup. Analisis kandungan kalsium dapat dilakukan dengan menggunakan AAS. Metode pengujian AAS pada bungkil kedelai belum memiliki metode baku penerapannya, sehingga perlu validasi metode. Metode Validasi metode meliputi pengujian linearitas, sensitivitas, presisi, dan akurasi. Hasil penelitian menunjukkan linearitas yang sangat baik pada rentang 0–6 mg/L dengan nilai r² sebesar 0,9962, dengan nilai LOD dan LOQ masing-masing 0,08 mg/L dan 0,28 mg/L. Nilai RSD presisi berada di bawah batas 2/3 RSD Horwitz, dan recovery mencapai 98,68%. Metode ini dinyatakan valid dan layak untuk analisis rutin kalsium pada bungkil kedelai.ABSTRACT. This study was conducted to validate a method for the determination of calcium content in soybean meal using Atomic Absorption Spectrophotometry (AAS) in accordance with ISO/IEC 17025:2017. Calcium is an essential mineral for living organisms. The analysis of calcium content can be performed using AAS. However, the application of the AAS method for soybean meal has not yet been standardized; therefore, method validation is required. The method validation parameters included linearity, sensitivity, precision, and accuracy. The results showed excellent linearity over the concentration range of 0–6 mg/L, with a coefficient of determination (r²) of 0.9962. The limit of detection (LOD) and limit of quantification (LOQ) were 0.08 mg/L and 0.28 mg/L, respectively. The relative standard deviation (RSD) for precision was below the 2/3 Horwitz RSD limit, and the recovery value reached 98.68%. Therefore, the method was considered valid and suitable for the routine analysis of calcium in soybean meal.
Co-Authors Hadianto, Ikhwan Ispitasari, Rina Kusumawati, Siska Devi Nur Latifah, Nur Nurlatifah