Trianggoro Budisulistyo
Neurology Department, Medical Faculty, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275

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The Effect of Moringa Leaves Extracts on MDA Level in Male Mice Exposed to Electromagnetic Radiation of Mobile Phones Nadia Ilmi Amalia; Trianggoro Budisulistyo; Dhega Anindita Wibowo; Tun Paksi Sareharto
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 13, No 4 (2024): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v13i4.43905

Abstract

Background: The use of cell phones causes anxiety about the effects of electromagnetic wave radiation emitted because of the production of free radicals in the body is increased by cell phone electromagnetic wave radiation. This escalation results in an elevated production of Reactive Oxygen Species (ROS), resulting in elevated Malondialdehyde (MDA) levels due to lipid peroxidation, which is toxic to the body. One way to reduce MDA levels is to introduce antioxidants into the body. Previous studies have found that Moringa leaf extract can be used as a good source of antioxidants.Objective: The aim of this research was to assess the impact of Moringa leaf extract administration on the MDA levels in BALB/C mice subjected to electromagnetic waves emitted by cell phones.Methods: This study employed an experimental study with a post-test only control group design. The research involved 23 male BALB/c mice, divided into four groups. The healthy control group received no treatment, while the negative control group was exposed to cell phone electromagnetic waves for 2 hours/day. Groups P1 and P2 received Moringa leaf extract at doses of 100 mg/kgBW and 400 mg/kgBW, respectively, in addition to being exposed to cellular phone electromagnetic waves for 2 hours/day. The treatment was administered for 30 days following a 7-dayadaptation period. On the 31st day, blood plasma samples were takenfrom all mice and examined for MDA levels. The data underwent analysis through non-parametric One-Way ANOVA and subsequent Pos Hoc LSD tests. Significance is acknowledged for p-values less than 0.05.Results: The highest MDA levels were found in the K (-) group, namely 0.43 ± 0.18 ppm, and the lowest MDA levels were observed in the P1 group, with values of 0.19 ± 0.08 ppm. A significant difference was noted between the K (-) group and the P1 group (p = 0.05), while no significant difference were observed in the other groups.Conclusion: Administering Moringa leaf extract at a dose of 100 mg/kgBW/day can reduce MDA levels in mice exposed to electromagnetic waves from cell phones.
The Effect of Moringa Leaves Extract on Sperm Motility in Male Mice Exposed to Electromagnetic Radiation of Mobile Phone Hasnaa Alyasi Sabrina; Trianggoro Budisulistyo; Achmad Zulfa Juniarto; Tun Paksi Sareharto
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 13, No 3 (2024): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v13i3.43904

Abstract

Background: Electromagnetic radiation can induce an increase in oxidative stress. The presence of oxidative stress can impact the structure of the plasma membrane of sperm cells, damage the structure of Deoxyribonucleic Acid (DNA), and accelerate the process of apoptosis, ultimately leading to a decline in sperm quality. This increase in oxidative stress can be prevented by substances that serve as antioxidants. Moringa plants, particularly the leaves, contain various substances that can act as antioxidants for the body. These include flavonoids, alkaloids, saponins, tannins, and terpenoids. The antioxidant content in Moringa leaves is believed to safeguard the process of spermatogenesisAim: To determine the impact of Moringa (Moringa oleifera) leaf extract on the motility of spermatozoa in BALB/C mice subjected to exposure to electromagnetic waves.Methods: This study utilized design featuring a post-test-only control group design, dividing participants into four randomly assigned groups, namely a negative control group, a positive control group (exposure to electromagnetic waves), and 2 treatment groups (moringa leaf extract at dose of 100 mg/kg BW and 400mg/kg BW). Each group compromised 5 experimental animals, and the treatments were administered for duration of 30 days. Spermatozoa preparations were made for each group and observed under a microscope (400x). Data were analyzed by initially testing for normality using the Shapiro-Wilk test. If the data distribution was normal, the hypothesis examination involved conducting a One Way ANOVA followed by subsequent Post Hoc testing.Results: The results of the one-way ANOVA test indicated differences between groups of mice (p<0.05). The post hoc test results further demonstrated that a dose of 400 mg/kg BW was the most effective in preventing a decrease in spermatozoa motility. This effect is believed to be atttributed to the presence of vitamin C, beta carotene, beta-sitosterol, flavonoids, and polyphenols.Conclusion: There is an improvement in spermatozoa motility in BALB/C mice exposed to electromagnetic waves after the administration of Moringa leaf extract.