Hermin Lastri Gintoe
Politeknik Cendrawasih Palu

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Eksplorasi Potensi Actinomycetes Penghasil Antibiotik Baru dari Danau Poso Sulawesi Tengah Sebagai Antibakteri Patogen pada Manusia Hermin Lastri Gintoe; Riskayati; Slamet Ifandi; Andi Lindhemutianingrum Siradje
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 8 No. 5 (2025): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/04h2zb36

Abstract

This study aims to isolate, characterize, evaluate antibacterial activity, and identify Actinomycetes isolates at the molecular level. The method used was an experimental approach with a descriptive design. The research consisted of several stages: (1) isolation and characterization through macroscopic, microscopic, and biochemical tests, and (2) antibacterial activity testing and molecular identification of Actinomycetes isolates. The results showed that five isolates—DP1, DP2, DP3, DP4, and DP5—were identified and suspected to belong to the genus Streptomyces. Macroscopic and microscopic characterization indicated that all isolates exhibited white, yellow, and brown colony colors and were Gram-positive. Biochemical tests showed positive results for catalase, coagulase, oxidase, citrate, and TSIA tests. Antibacterial activity assays against Escherichia coli and S. aureusrevealed that isolate DP4 had the strongest inhibition zone, while showing negative activity against S. typhi. In conclusion, five Actinomycetes isolates were successfully isolated from the sediment soil of Lake Poso. Isolate DP4 demonstrated the greatest potential as an antibacterial candidate by inhibiting two pathogenic bacteria. Based on 16S rDNA sequence similarity analysis, isolate DP4 was identified as S. aurantiacus with 99% similarity. Keywords: Actinomycetes, Antibacterial, Lake Poso, Exploration
Penyuluhan dan Pemeriksaan Kadar Asam Urat (dalam Darah) pada Masyarakat Desa Bangga Kec. Dolo Selatan, Kab. Sigi, Prov Sulawesi Tengah Hermin Lastri Gintoe; Andi Lindhemuthianingrum; Astri Febriana Iffaf
ALKHIDMAH: Jurnal Pengabdian dan Kemitraan Masyarakat Vol. 2 No. 4 (2024): Oktober: Jurnal Pengabdian dan Kemitraan Masyarakat
Publisher : LP3M INSTITUT KH YAZID KARIMULLAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59246/alkhidmah.v2i4.1118

Abstract

This activity aims to increase public knowledge about gout and conduct uric acid level checks in Bangga Village, Dolo Selatan District, Sigi Regency, Central Sulawesi Province. The main problem faced is the high incidence of gout, which is often caused by unhealthy eating patterns and lack of knowledge about gout. The methods used include interactive counseling and uric acid level checks with the Point of Care Testing (POCT) tool, carried out on January 8, 2024. Of the 15 participants who took part in the activity, 9 people were found to have high uric acid levels and 6 people with normal levels. The results showed that most of the respondents who had high uric acid levels came from the older age group. This activity succeeded in increasing public awareness of the importance of a healthy diet and is expected to encourage behavioral changes in maintaining health.