This study aims to determine the effect of work discipline and work environment on the performance of Puskesmas Pesanggrahan South Jakarta employees. The research method used is descriptive associative with a total sample population of 64 respondents. Methods of data collection using a questionnaire. The analysis used includes: validity test, reliability test, classical assumption test, correlation coefficient test, coefficient of determination test, simple linear regression test, t-test and F-test. The results of the partial test (t-test) obtained that work discipline and work environment partially have a significant effect on employee performance. The results of the simultaneous test show that discipline affects the performance of the regression equation Y = 17.934 + 0.549X1, the correlation coefficient is 0.613, meaning that the two variables have a strong relationship. The value of determination or contribution of influence is 0.624 or 62.4% the work environment affects the performance of the regression equation Y = 21.127 + 0.465X2, the correlation coefficient of 0.593 means that the two variables have a strong relationship level. The value of determination or contribution of influence is 0.649 or 64.9% work discipline and work environment together have a significant effect on employee performance with a calculated F value > F table or (36.831 > 2.760). This is reinforced by the multiple linear regression equation Y = 18.224 + 0.395X1 + 0.147X2. While the contribution of work discipline and work environment variables to employee performance is 57.6%.