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All Journal Journal of Biodiversity and Biotechnology
Iswahyudi Iswahyudi
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret (UNS), Jl. Ir. Sutami 36A, Kentingan, Surakarta, 57126, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry

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Growth Response and Yields of Variety of Sorghum on Multiple NPK Fertilization in Dry Land Samanhudi Samanhudi; Puji Harsono; Sri Hartati; Iswahyudi Iswahyudi; Naufal Ghazy Wicaksono
Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.108195

Abstract

Land degradation is a major constraint to agricultural productivity, with drylands particularly vulnerable to critical conditions. Sorghum (Sorghum bicolor L. Moench) is a promising crop for such environments due to its adaptability and dual-purpose value for both biomass and sugar-rich stalks. This study evaluated the growth and yield response of four sorghum varieties—Super, Suri, Numbu, and Keller—to different rates of compound NPK fertilizer under dryland conditions. The experiment was arranged in a Randomized Complete Block Design (RCBD) with two factors: sorghum varieties and five fertilizer doses (0, 10, 12, 14, and 16 g plant⁻¹), replicated three times. Data were subjected to ANOVA, and treatment means were compared using DMRT at the 5% significance level. Results showed that the Keller variety consistently produced the best agronomic performance, including plant height, number of leaves, stem segments, stem diameter, and biomass dry weight. Moreover, Keller combined with 12 g plant⁻¹ of NPK fertilizer produced the highest sap content (14.0% Brix), highlighting its potential as a dual-purpose sorghum. Leaf traits were most responsive at 14 g plant⁻¹, indicating that moderate-to-high fertilization enhances vegetative growth without excessive input. These findings demonstrate that varietal selection coupled with appropriate nutrient management is crucial for optimizing sorghum production in dryland systems. The study provides practical guidance for farmers and can inform future breeding and fertilizer recommendation programs for sustainable sorghum cultivation.
In Vitro Effects of Yeast Extract and Indole-3-Acetic Acid on Shoot Emergence and Height in Mas Kirana Banana Samanhudi Samanhudi; Retna Bandriyati Arniputri; Sri Hartati; Iswahyudi Iswahyudi; Dewi Selvia Maulida Saputri
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.108197

Abstract

Mas Kirana banana (Musa sp.) is a superior local variety from Lumajang, East Java, valued for its favorable fruit size and consumer preference. Conventional propagation of this variety is limited due to its low production of saplings (2–3 per clump), making large-scale cultivation inefficient. Tissue culture techniques offer an alternative approach to accelerate propagation. This study aimed to evaluate the effects of yeast extract and indole-3-acetic acid (IAA) on the in vitro growth of Mas Kirana banana explants. The experiment was arranged in a factorial completely randomized design (CRD) with two factors: yeast extract at four concentrations (0, 400, 800, and 1200 mg L⁻¹) and IAA at four concentrations (0, 0.75, 1.5, and 2.25 ppm). Growth responses were assessed based on shoot emergence, shoot height, root development, and leaf formation. Yeast extract significantly affected shoot emergence time; however, its effect was inhibitory rather than promotive. The control (0 mg L⁻¹) produced the fastest emergence (11 DAP), whereas higher yeast concentrations (400–1200 mg L⁻¹) delayed emergence to 13–22 DAP.. IAA significantly influenced shoot height, with the best performance at 1.5 ppm, while other growth parameters were not significantly affected. These findings indicate that the effectiveness of yeast extract and IAA is highly concentration-dependent, and their roles in improving micropropagation of Mas Kirana banana require further optimization. This study provides baseline information to support the development of more efficient tissue culture protocols for large-scale propagation of this important local variety.
Co-Authors Dewi Selvia Maulida Saputri Naufal Ghazy Wicaksono Puji Harsono Retna Bandriyati Arniputri Samanhudi Samanhudi Samanhudi Samanhudi Sri Hartati Sri Hartati