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All Journal Jurnal Pengabdian Masyarakat (ABDIRA) Journal of Innovative and Creativity
Mujtahid Bin Abd. Kadir
Universitas Megarezky
Aerospace Engineering Computer Science & IT Education Languange, Linguistic, Communication & Media Mathematics Nursing Social Sciences Other

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The Effect of Tissue Fixation Duration on the Quality of Histopathological Examination Results at Sawerigading Palopo Regional General Hospital Nurhilaliyah; Mujtahid Bin Abd. Kadir; Muhajirah; Muthahharah; Nirmala
Journal of Innovative and Creativity Vol. 6 No. 1 (2026)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v6i1.6596

Abstract

This study aimed to evaluate the effect of fixation duration on the quality of histopathological preparations of prostate tissue using Hematoxylin and Eosin (H&E) staining. A quantitative quasi-experimental design was employed using prostate tissue obtained from biopsy specimens at Sawerigading Palopo Regional General Hospital. Ten tissue samples were subjected to three different fixation durations 6 hours, 24 hours, and 48 hours resulting in 30 observational units. All specimens were fixed in 10% formalin and processed through standard histopathological procedures, including dehydration, clearing, embedding, sectioning, and H&E staining. Histological quality was assessed microscopically based on five parameters: staining quality, nuclear sharpness, cytoplasmic integrity, presence of artifacts, and clarity of histological structure. Data were analyzed using descriptive statistics and inferential tests, including normality testing and nonparametric correlation analysis. The results showed that fixation duration significantly affected histopathological quality. Specimens fixed for 6 hours demonstrated suboptimal morphology characterized by poor nuclear clarity and cytoplasmic integrity, whereas 24 hour fixation produced markedly improved histological features. The highest quality was observed in specimens fixed for 48 hours, showing optimal staining, clear nuclear detail, and minimal artifacts. Statistical analysis revealed a strong positive correlation between fixation duration and histological quality (p < 0.05). These findings indicate that fixation duration plays a critical role in determining the quality of prostate histopathological preparations, and a fixation period of 24–48 hours is recommended to obtain optimal diagnostic results.
Upaya Pencegahan Penyebaran Covid-19 dan Penyakit Menular melalui Pembuatan Hand Sanitizer Kun Ika Nur Rahayu; Devangga Darma Karingga; Mujtahid Bin Abd. Kadir
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 6, No 3 (2026): Abdira
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v6i3.2007

Abstract

Communicable diseases including COVID-19 remain a public health problem that requires prevention efforts based on community empowerment. This community service activity aimed to improve the knowledge and skills of the community in the working area of Sukorame Public Health Center, Kediri City, in producing hand sanitizer containing 60% alcohol with aloe vera as an additional ingredient. The method used was an educational-participatory approach through health education, discussion, demonstration, hands-on practice, mentoring, and evaluation using pre-tests, post-tests, and skill observation. The results showed an increase in the good knowledge category from 17.8% to 77.8% after the education session. In addition, 84.4% of participants were able to independently produce hand sanitizer according to the procedure. This activity shows that education and practical training are effective in improving community capacity for preventing communicable diseases. The results of this community service are important as a form of community empowerment to support sustainable clean and healthy living behavior.
Co-Authors Karingga, Devangga Darma Kun Ika Nur Rahayu Muhajirah Muthahharah Nirmala Nurhilaliyah