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Phytochemical Profiling and TLC Analysis of Hymenocardia acida Methanol Leaf Extract Zubair, Abdulgafar Omeiza
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1399-1401

Abstract

The study investigated the phytochemical constituents and chromatographic profile of Hymenocardia acida leaf extract obtained through methanol extraction. Hymenocardia acida, widely used in traditional medicine across Africa, is reputed for its therapeutic potential in treating various ailments. To explore the bioactive compounds, we conducted a series of qualitative phytochemical screenings to detect the presence of alkaloids, flavonoids, saponins, tannins, phenols, terpenoids, steroids. The results revealed a broad spectrum of phytochemicals known to exhibit antioxidant, antimicrobial, and anti-inflammatory properties. Additionally, thin-layer chromatography (TLC) was performed to obtain a chromatographic profile of the methanol extract, aiming to separate and identify potential bioactive compounds. Silica gel plates were used as the stationary phase, and various solvent systems, such as hexane acetate and chloroform were optimized for effective separation of phytochemicals. The resulting TLC plates were analyzed under UV light at 254 nm and 365 nm, and retention factor (Rf) values were calculated to provide preliminary identification. Different solvent systems were used which showed different behaviors in the plant compounds. The resulting mixture between hexane and ethyl acetate at ratio 9:1, 8:2, 7:3, 6:4 and 5:5 are given as (0), (0,0.26,0.16), (0,0.95,0.94), (0) and (0,0.91,0.95) respectively. The TLC fingerprint and phytochemical profile generated from this study offer valuable insights into the potential pharmacological applications of Hymenocardia acida, supporting its use in traditional medicine and guiding further isolation and characterization of its active compounds for drug development.
Bacteriological Quality of Raw Cow Milk Obtained from Fulanis Within University of Abuja Environment Zubair, Abdulgafar Omeiza; Zubair, Jemilat Oyiza; Muhammed, Rofiat Oyine
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.555-559

Abstract

Milk is a highly nutritious food that can serve as a suitable medium for microbial growth if not properly handled. This study assessed the bacteriological quality of raw cow milk obtained from Fulani herders within the University of Abuja environment with emphasis on the prevalence of bacterial species and their potential public health implications. Twenty raw milk samples were collected and analyzed using standard microbiological techniques, including serial dilution and spread plate methods for enumeration, determination of total bacterial load and total coliform count, Gram staining and biochemical identification. Five bacterial species were isolated; Bacillus spp. (34.29%), Lactobacillus spp. (28.57%), Staphylococcus aureus (17.14%), Pseudomonas aeruginosa (11.43%) and Escherichia coli (8.57%). The total bacterial load ranged from 5.5 × 108 to 1.15 × 109 CFU/mL, while total coliform counts ranged from 3.35 × 105 to 8.40 × 105 CFU/mL. The predominance of Gram-positive bacteria such as Bacillus and Lactobacillus reflects the natural microbial flora of raw milk, whereas the presence of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli indicates contamination from handlers, equipment or the environment. These findings reveal significant microbial contamination and associated public health risks linked to raw milk consumption. The study underscores the need for improved hygienic practices during milking, handling, and storage to reduce contamination and ensure consumer safety.