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All Journal Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Journal of Community Service and Empowerment
Wahyu Prihanta
Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang, Indonesia
Agriculture, Biological Sciences & Forestry Education Social Sciences Other

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The strength of Muhammadiyah's existence in the modern era: Pillars of empowerment and community strengthening Wahyu Prihanta; Moh. Nurhakim; Saiful Amien
Journal of Community Service and Empowerment Vol. 5 No. 2 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v5i2.33085

Abstract

A study regarding the role of Muhammadiyah in the pillars of community empowerment and strengthening needs to be carried out, considering that this organization is the largest organization in Indonesia. This article aimed to analyze the strength of Muhammadiyah's existence which focuses on the pillars of empowerment and strengthening of society. This paper was prepared using library research methods. Data were analyzed using qualitative descriptive analysis. Data visualization, especially regarding references and keywords that frequently appear, is assisted with VOSviewer. The results show that Muhammadiyah is an Islamic organization that has a clear and real role in empowering and strengthening of society. Muhammadiyah has a central role in the field of philanthropy, in fact this has become Muhammadiyah's identity. Al-Ma'un became the ideological-theological foundation of Muhammadiyah's struggle. The advocacy movement through community empowerment has changed the face of Muhammadiyah to become more populist. Community empowerment is carried out through action programs that directly touch on the real problems of poor and marginalized communities represented by farmers, fishermen, livestock breeders, workers, urban poor communities and people with disabilities. Women's groups who have been marginalized and tend to be subordinated have also become targets of empowerment work carried out by Muhammadiyah, especially through its wing organization, namely Aisyiyah.
In silico and in vitro analysis primers for antibiotic resistance gene of bacteria Eva Ayuntya; Kiky Martha Ariesaka; Wahyu Prihanta; Husamah Husamah; Fendy Hardian Permana; Moh Mirza Nuryady
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4313

Abstract

The prevalence of Extended-Spectrum Beta-Lactamase (ESBL) occurs due to excessive use of antibiotics, resulting in antibiotic resistance. Research on ESBL has been conducted in several Indonesian cities, such as Surabaya, Malang, and Jakarta which identified the presence of ESBL bacterial isolates. Limited information about blaCTX-M and blaVEB primer data in in silico analysis such as primer length, GC base content, melting temperature, and organisms attached to the gene, is an important thing that needs further research, because in silico data will affect the annealing temperature in the in vitro process using Polymerase Chain Reaction (PCR). The purpose of this study was to determine that blaCTX-M and blaVEB primers can be used to detect the presence of ESBL genes from amplification results in PCR. In silico primer analysis was carried out using BLAST primers from the NCBI website which would then be continued through in vitro primer analysis using PCR to see the presence of DNA bands. The results of in silico research showed that there were 27 organisms detected in the blaCTX-M gene and 37 organisms in the blaVEB gene. The results of in vitro analysis on blaCTX-M primers can be amplified at ± 400 bp, while blaVEB cannot be amplified. 
Co-Authors Eva Ayuntya Fendy Hardian Permana Husamah Husamah Kiky Martha Ariesaka Moh Mirza Nuryady Moh. Nurhakim Saiful Amien