Juwita Cristina Situmeang
UNIVERSITAS NEGERI MEDAN

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN JERUK PURUT (CITRUS HYSTRIX) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS Naomi Putri Chelsea Solin; Juwita Cristina Situmeang; Novia Christy Nababan
Didaktik : Jurnal Ilmiah PGSD STKIP Subang Vol. 11 No. 04 (2025): Volume 11 No. 04 Desember 2025 Published
Publisher : STKIP Subang

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Abstract

The increasing incidence of infections caused by Staphylococcus aureus and the growing problem of antibiotic resistance have encouraged the exploration of natural sources as alternative antibacterial agents. Kaffir lime (Citrus hystrix) leaves are known to contain various secondary metabolites with potential antibacterial properties. This study aimed to evaluate the antibacterial activity of kaffir lime leaf extract against Staphylococcus aureus and to analyze the effect of different extract concentrations on the diameter of inhibition zones. The extract was prepared using a maceration method with 96% ethanol, followed by solvent evaporation to obtain a concentrated extract. Antibacterial activity was assessed using the disc diffusion method with extract concentrations of 5%, 10%, 15%, and 20%. Chloramphenicol was used as a positive control, while 96% ethanol served as a negative control. The results showed that all extract concentrations produced inhibition zones ranging from 6.24 to 6.62 mm, which were classified as weak antibacterial activity and did not demonstrate a significant concentration-dependent effect. In contrast, the positive control exhibited a markedly larger inhibition zone of 32.14 mm, while the negative control showed no inhibitory effect. These findings indicate that the ethanol extract of kaffir lime leaves exhibits limited antibacterial activity against Staphylococcus aureus under the experimental conditions. Further studies involving extraction optimization or purification of active compounds are recommended to enhance its antibacterial potential.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN JERUK PURUT (CITRUS HYSTRIX) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS Naomi Putri Chelsea Solin; Juwita Cristina Situmeang; Novia Christy Nababan
Didaktik : Jurnal Ilmiah PGSD STKIP Subang Vol. 11 No. 04 (2025): Volume 11 No. 04 Desember 2025 Published
Publisher : STKIP Subang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The increasing incidence of infections caused by Staphylococcus aureus and the growing problem of antibiotic resistance have encouraged the exploration of natural sources as alternative antibacterial agents. Kaffir lime (Citrus hystrix) leaves are known to contain various secondary metabolites with potential antibacterial properties. This study aimed to evaluate the antibacterial activity of kaffir lime leaf extract against Staphylococcus aureus and to analyze the effect of different extract concentrations on the diameter of inhibition zones. The extract was prepared using a maceration method with 96% ethanol, followed by solvent evaporation to obtain a concentrated extract. Antibacterial activity was assessed using the disc diffusion method with extract concentrations of 5%, 10%, 15%, and 20%. Chloramphenicol was used as a positive control, while 96% ethanol served as a negative control. The results showed that all extract concentrations produced inhibition zones ranging from 6.24 to 6.62 mm, which were classified as weak antibacterial activity and did not demonstrate a significant concentration-dependent effect. In contrast, the positive control exhibited a markedly larger inhibition zone of 32.14 mm, while the negative control showed no inhibitory effect. These findings indicate that the ethanol extract of kaffir lime leaves exhibits limited antibacterial activity against Staphylococcus aureus under the experimental conditions. Further studies involving extraction optimization or purification of active compounds are recommended to enhance its antibacterial potential.
TREN DAN ARAH PENDIDIKAN PERUBAHAN IKLIM DAN PEMBELAJARAN IPA: TINJAUAN LITERATUR SISTEMATIS Juwita Cristina Situmeang; Helen Veronita Hutapea; Imelda Situmorang
Didaktik : Jurnal Ilmiah PGSD STKIP Subang Vol. 12 No. 02 (2026): Volume 12 No. 2, Juni 2026 Publish
Publisher : STKIP Subang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36989/didaktik.v12i01.12354

Abstract

ABSTRACT This study aims to analyze research trends and directions in climate change education within science learning through a Systematic Literature Review (SLR) approach. Climate change has become a critical global issue that requires not only technological solutions but also educational interventions to improve students’ scientific literacy and environmental awareness. This study adopted the PRISMA 2020 framework, consisting of identification, screening, eligibility, and inclusion stages. A total of 500 articles were initially identified from Google Scholar and ResearchGate using Publish or Perish, with keywords “Climate Change Education” and “Science Learning” (2015–2025). After a rigorous selection process, 12 articles met the inclusion criteria, and 6 articles were analyzed in depth. The data were analyzed using bibliometric analysis with VOSviewer, synthesis matrix, and Critical Journal Review (CJR). The findings indicate that research trends are dominated by themes such as climate education and student engagement, scientific literacy and sustainability, inquiry-based learning, family involvement, and climate impact studies. Furthermore, the results reveal that integrating climate change topics into science learning enhances students’ critical thinking skills, environmental awareness, and engagement in real-world problem solving. However, research gaps remain, particularly in the integration of climate change education into formal curricula, experimental studies, and research in developing countries. This study provides important insights for educators, researchers, and policymakers in developing more effective and contextual science learning related to climate change.