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All Journal Green and Tropical Laboratory for Sustainability
Moh. Mirza Nuryady
Department of Biology Education, Faculty of Teacher and Training Education, Universitas Muhammadiyah Malang, Indonesia
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Environmental Science Social Sciences

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Immunostimulanting effect of jackbean flour on non-specific immunity of mice in vitro and in silico Riris Dewi Retnowati; Moh. Mirza Nuryady; Elly Purwanti; Sri Wahyuni; Iin Hindun
Green and Tropical Laboratory for Sustainability Vol. 1 No. 1 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v1i1.36289

Abstract

Background: Jackbean (Canavalia ensiformis) contains protein and secondary metabolite compounds, such as phenolics and flavonoids that can have potential as antioxidants, antibacterial, antimicrobial, and antiviral. Objectives: This study aims to determine the effect of giving jackbean flour as an immunostimulant on non-specific immunity of mice in-vitro and in-silico. Methods: This type of research is double methods research with a comparative method and uses an experimental design in the form of a Randomized Group Design and in-silico testing with the NCBI website. Mice with a total of 16 heads were divided into 4 treatment groups, namely the Negative Control group (P0), Dose 1 (P1) with a solution of jackbean flour as much as 2.3 g, Dose 2 (P2) as much as 4.6 g, Dose 3 (P3) as much as 9.2 g. The hemagglutination test was carried out on mice with a total of 16 mice. The hemagglutination test was carried out on the 15th day after giving the flour solution, then tested the content of active compounds in koro pedang in silico according to the literature review of previous research from the website which contains active compounds, namely canavanine, concanavalin A and B. Results: The results of in-vitro research show that the most influential dose for hemagglutination is P1 with a dose of 2.3 grams, characterized by almost all wells on the microplate there is concentrated clotting, and there is significance (p < 0.05) which means there is a difference in the effectiveness of jackbean flour solution as an immunostimulant from each dose. Conclusion: This study concluded that jack bean flour, especially at a dose of 2.3 grams, effectively enhanced non-specific immunity in mice, as evidenced by significant hemagglutination results. Therefore, jack bean flour has the potential to be a natural immunostimulant in improving immune health and fighting infections.
Neem leaves (Azadirachta Indica) and Jasmine flowers (Jasminum sambac): A toxic combination of Aedes aegypti Putri Ayu Irodah; Moh. Mirza Nuryady; N. Nurwidodo
Green and Tropical Laboratory for Sustainability Vol. 1 No. 1 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v1i1.36335

Abstract

Background: The high number of cases of dengue hemorrhagic fever (DHF) in Indonesia indicates the lack of diseases prevention caused by mosquito vectors. Chemical control efforts can cause resistance to mosquitoes, so it is necessary to have natural insecticides derived from plants, one of which is neem and jasmine which has active ingredients as larvicides. Objectives: This research aims to analyze the effect of different concentrations of extracts from neem (Azadirachta indica) and jasmine (Jasminum sambac) leaves on the mortality of Aedes aegypti larvae. Methods: This research is true experimental research using 7 treatments, there are negative control group using distilled water and a positive control group using 1% abate and 5 treatment groups using combination of extracts from neem leaves (Azadirachta indica) and jasmine flower (Jasminum sambac) with concentration ratio 0%:100%, 25%:75%, 50%:50%, 75%:25%, dan 100%:0%. In this study, it is used Aedes aegypti larvae taken by landing collection in Nguling, Pasuruan. Results: The results of identification of larvae showed that the larvae taken were Aedes aegypti larvae in terms of morphological characteristics. The results showed that the combination of 50%: 50% concentration caused a lot of mortality in larvae and obtained LC50 from the extract of neem (Azadirachta indica) and jasmine flower (Jasminum sambac) was 253,57 µg / mL. Conclusion: Extracts from neem leaves (Azadirachta indica) and jasmine flowers (Jasminum sambac) can be used as an candidate alternative to Aedes aegypti larvicide.
Aedes aegypti mosquitoes in Malang Regency: What is the resistance status to malathion 0.8%? Jihan Ully Agustin; Siti Zaenab; Moh. Mirza Nuryady; Tutut Indria Permana
Green and Tropical Laboratory for Sustainability Vol. 1 No. 1 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v1i1.36354

Abstract

Background: Dengue hemorrhagic fever (DHF) carried by the Aedes aegypti has become a health problem in Karangploso, Singosari, and Lawang Districts. The vector control program implemented by the government is fogging. Fogging contains an active material malathion which can hit adult mosquitoes. Excessive use of malathionic active ingredients can lead to vector resistance. Objectives: The purpose of this study is to analyze the resistance status of Ae. aegypti to malathion 0.8% in Karangploso, Singosari, and Lawang Districts, as well as community behavior in controlling dengue disease. Methods: Type of research is a descriptive observational study with cross sectional approach. Data was obtained through susceptibility tests in the laboratory and observations of public attitudes in controlling dengue disease through a Google form survey. The data was presented in tabular form and discussed descriptively. The results showed that the mortality percentage of Ae. aegypti mosquitoes in Karangploso District was 98%, Singosari 96%, and Lawang 98%, all of which were in the tolerant category. Results: The results of observations to the community showed that the community already knew the most common breeding sites for Ae. aegypti larvae is bathub and the way people control dengue fever using several types of household insecticides, namely spray, burn, and anti-mosquito lotions. Conclusion: The conclusion of this study is the resistance status of Ae. aegypti in the three sub-districts are classified as tolerant and the community already knows the most preferred breeding sites for the Ae. aegypti, but the way people control DHF is still using some household insecticides that may increase the status of Ae. aegypti becomes resistant.
Co-Authors Elly Purwanti Iin Hindun Jihan Ully Agustin N. Nurwidodo Putri Ayu Irodah Riris Dewi Retnowati Siti Zaenab Sri Wahyuni Tutut Indria Permana