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Mapping Plant Biotechnology Research for Low Heavy Metal Accumulation and Crop Safety: A Bibliometric–SLR Study Highlighting CRISPR-Cas9 Zakiyah, Nur Meili; Sanjaya, Bella Rhea Lavifa; Sutini, Sutini
G-Tech: Jurnal Teknologi Terapan Vol 10 No 1 (2026): G-Tech, Vol. 10 No. 1 January 2026
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/g-tech.v10i1.8732

Abstract

Heavy metal contamination in agriculture presents a growing risk to food security, underscoring the need for precise and scalable biotechnological solutions. This study maps the research landscape of plant biotechnology for reducing heavy metal accumulation using an integrated bibliometric analysis (Bibliometrix/Biblioshiny and VOSviewer) combined with a PRISMA-based SLR of 43 English-language empirical articles published between 1997 and 2025. The analysis identifies four major thematic clusters: tolerance mechanisms, field applications, multi-omics, and genome editing that indicating a shift from early physiological studies toward precision biotechnology. The focused SLR reveals that CRISPR-Cas9, although still limited in publication volume, has emerged as a strategically positioned research front, particularly in recent crop-focused genome-editing studies involving agriculturally relevant species. Geographical patterns show strong research productivity in Asia and high scientific influence in Western countries. Despite the modest dataset, this study provides a strategic roadmap for prioritizing CRISPR applications, strengthening interdisciplinary integration, and accelerating biotechnology-based solutions for food safety and sustainable agriculture.
Phenological Stage Application of Gibberellin to Enhance Fruit Quality of Melon (Cucumis melo ‘Fujisawa’) under NFT Hydroponic System Hidayat, Ramdan; Fasya, Elfira Rizki Oktaviana; Nugrahani, Pangesti; Zakiyah, Nur Meili
AGRIVITA Journal of Agricultural Science Vol 48, No 2 (2026): IN PRESS
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.5140

Abstract

National melon production remains suboptimal due to the crop’s sensitivity to the environment and lack of management practices. This study analyzed the interaction between GA₃ concentration (0, 50, 100, 150 ppm) and application timing (pre-anthesis, anthesis, post-anthesis) on growth, yield, and fruit quality of Fujisawa melon in an NFT hydroponic system. Significant concentration × timing interactions (p<0.05) optimized fruit traits at 100 ppm + anthesis: weight increased by 0.49 kg (24.1%) to 2.03 kg, flesh thickness by 1.33 cm (25.7%) to 5.17 cm, diameter by 1.79 cm (10.9%) to 16.38 cm, and volume by 413 cm³ (21.2%) to 1,950 cm³ vs. control. Regardless of timing, 100 ppm GA₃ accelerated flowering (2.71 days) and harvest (3.87 days) vs. control and increased total soluble solids by 2.33 to 16.33ºBrix. Pre-anthesis application enhanced plant length by 8.57–9.41 cm and hastened flowering by 0.87–0.99 days compared to other timings. Leaf number and fruit cavity were unaffected by either treatment (p>0.05). The regression for fruit traits at anthesis (R²=0.74–0.85) confirmed optimum concentrations of 67.50–138.50 ppm; ≥150 ppm diminished benefits. These findings provide recommendations: 100 ppm GA₃ at anthesis maximizes yield components, 75 ppm at flexible times increases sweetness levels.