TRUC, NGUYEN TRUNG
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Antifungal potential of Enydra fluctuans extract for postharvest control of Colletotrichum siamense in dragon fruit TRUC, NGUYEN TRUNG; THI, QUACH VAN CAO; NHUNG, DUONG THI CAM
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100127

Abstract

Abstract. Truc NT, Thi QVC, Nhung DTC. 2026. Antifungal potential of Enydra fluctuans extract for postharvest control of Colletotrichum siamense in dragon fruit. Asian J Agric 10 (1): g100127. https://doi.org/10.13057/asianjagric/g100127. Pitaya, commonly known as red-fleshed dragon fruit, is rich in bioactive and nutritional compounds; however, its postharvest shelf life is limited due to high susceptibility to fungal diseases, particularly anthracnose caused by Colletotrichum spp. These infections significantly reduce fruit quality, shorten storage duration, and decrease economic value. This study investigated the antifungal efficacy of Whole Enydra fluctuans Phenolic Extract (WEPE) against postharvest pathogens of dragon fruit under in vitro and in vivo conditions. Fifteen fungal isolates were recovered from infected fruits, among which strain TL12 was identified as Colletotrichum siamense based on morphological characteristics and ITS rDNA sequencing. Pathogenicity tests confirmed Koch’s postulates, producing disease symptoms comparable to those observed in naturally infected fruits. To the best of our knowledge, this is the first report of C. siamense associated with postharvest anthracnose of dragon fruit in the studied region. WEPE obtained using ultrasound-assisted extraction with 45% ethanol exhibited the highest total phenolic content (18.26±1.49 mg GAE/g). In vitro assays demonstrated strong antifungal activity, with minimum inhibitory concentrations of 700 μg/mL for complete mycelial growth inhibition and 5000 μg/mL for spore germination inhibition. In vivo application of WEPE significantly suppressed anthracnose development on dragon fruit in a concentration-dependent manner. After 7 days of incubation, disease severity was reduced by approximately 30.4% and 55% at 700 and 5000 μg/mL, respectively, compared to the control. The results indicate that WEPE possesses strong antifungal potential against C. siamense and may be considered a promising natural agent for postharvest disease management in dragon fruit. However, these findings are based on short-term storage experiments under controlled laboratory conditions. Further research involving formulation optimization, extended storage evaluation, and field-scale validation is necessary before practical application in commercial postharvest systems.
Sodium alginate-coconut oil edible coating delayed postharvest senescence and enhanced antioxidant capacity of lemon (Citrus latifolia) fruit TRUC, NGUYEN TRUNG; NHUNG, DUONG THI CAM
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100156

Abstract

Abstract. Truc NT, Nhung DTC. 2026. Sodium alginate-coconut oil edible coating delayed postharvest senescence and enhanced antioxidant capacity of lemon (Citrus latifolia) fruit. Asian J Agric 10 (1): g100156. https://doi.org/10.13057/asianjagric/g100156. Postharvest quality deterioration of lemon fruit is closely associated with physiological metabolism and oxidative stress during storage. This study investigated the efficacy of Sodium Alginate (SA) and Coconut Oil (CO) coatings, applied individually or in combination, in regulating physiological metabolism, oxidative stress, and antioxidant defense responses of lemon fruit during ambient storage. Fruits were assigned to four treatments (Control, SA, CO, and SA-CO) using a completely randomized design with three replicates per treatment. Coated and uncoated fruits were stored at 25℃ for 18 days, and key physiological (moisture loss, color), biochemical (vitamin C, total phenolics), reactive oxygen species (ROS), and antioxidant enzyme indicators were periodically evaluated. Relative to the control, the combined SA-CO coating markedly delayed deterioration. By day 18, SA-CO treated fruit showed weight loss of about 14%, which was roughly 2.5 percentage points lower than the control. Chlorophyll degradation was reduced, with SA-CO fruit retaining around 185 mg kg-1 chlorophyll, approximately 40 mg kg-1 higher than the control. Antioxidant compounds were better preserved, with vitamin C maintained at nearly 0.29 g kg-1 and total phenolics reaching about 0.15 g GAE kg-1, both slightly higher than the control. The coating also improved antioxidant enzyme regulation. At the end of storage, SOD activity in SA-CO fruit was close to 29 U mg-1 protein, substantially lower than the control, whereas APX and CAT activities increased by about 12 U mg-1 and 25 U mg-1 protein, respectively. Correspondingly, reactive oxygen species decreased, with superoxide radicals recorded at around 1.5 mmol NO₂ kg-1 s-1 and hydrogen peroxide at about 0.8 µmol H₂O₂ kg-1, both noticeably lower than the control. Overall, the combined SA-CO coating most effectively mitigated physiological degradation, improved antioxidant defense responses, and reduced oxidative stress, thereby supporting better retention of postharvest quality in lemon fruit stored under ambient conditions.