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All Journal Jurnal Rekayasa Lingkungan
Rachman, Mahyudin Abdul
BPPT
Agriculture, Biological Sciences & Forestry

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PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN Rachman, Mahyudin Abdul
Jurnal Rekayasa Lingkungan Vol 6, No 1: Jurnal Rekayasa Lingkungan
Publisher : BPPT

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Abstract

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12,13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase defficient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methanesludge
ISOLASI DAN IDENTIFIKASI BAKTERI LIMBAH BIODIESEL PENGHASIL BIO-ENERGI DAN BIO-MATERIAL Rachman, Mahyudin Abdul
Jurnal Rekayasa Lingkungan Vol 5, No 2: Jurnal Rekayasa Lingkungan
Publisher : BPPT

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Glycerol from the waste of biodiesel can be utilized to 1,3-propanediol (PDO) and hydrogen gas (H2) with biotransformation processing. The product of PDO will be used as raw material for polymer, polyurethane and polyester and H2 will be converted to electrical energy by using Fuel-cell. The anaerobe or aerobe bacterium which has ability to degrade biodiesel waste for environmental friendly such as genus Enterobacter, Klebsiella, Clostridium, Bacillus, Citrobacter, etc. The direct screening of bacteria for isolation from biodiesel waste in order to obtain the bacteria which has highest conversion of glycerol to PDO and H2. We have found that 28 species from liquid and 7 from solid biodiesel waste and by using IMVIC testing, 7 species of bacteria was Klebsiella or Enterobacter which can be predicted to convert glycerol for PDO and H2. The highest yield product was species TH3from biodiesel waste. Moreover, the microbiology identification indicated that the strain TH3 was Klebsiella sp. strain 23 with 98 % similarity.Key words : Waste of biodiesel, Glycerol, PDO, H2and bacteria.
PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN Rachman, Mahyudin Abdul
Jurnal Rekayasa Lingkungan Vol 5, No 1: Jurnal Rekayasa Lingkungan
Publisher : BPPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.328 KB)

Abstract

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12, 13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase deffi cient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methane sludge
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