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Ami Febriza Achmad
Department of Physiology, Faculty Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Department of Surgery, Faculty Medicine and Health Sciences, Universitas Muhammadiyah Makassar
Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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In Vitro Testing of the Antibacterial Activity of Ethanol Extract of Lontar Leaves (Borassus flabellifer) Against Staphylococcus aureus Ami Febriza Achmad; Asdar Tajuddin; Rifqah Amaliah; Rolly Riksanto Bahtiar
Medica Hospitalia : Journal of Clinical Medicine Vol. 12 No. 3 (2025): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36408/mhjcm.v12i3.1228

Abstract

BACKGROUND: Staphylococcus aureus is a bacterium that frequently causes infections and often develops resistance to antibiotics. Efforts to identify alternative treatments using herbal remedies are increasing. In Indonesia, a country rich in biodiversity, lontar leaves (Borassus flabellifer) have been traditionally used and show potential antibacterial activity. AIMS: To evaluate the antibacterial activity of ethanol extract of lontar leaves against Staphylococcus aureus in vitro. METHOD: This experimental study employed a post-test-only control group design. Antibacterial activity was tested using the well diffusion method. Extracts were obtained through maceration with 96% ethanol and tested at 75%, 50%, and 25% concentrations. Ciprofloxacin was used as the positive control, and 10% DMSO as the negative control. RESULTS: The inhibition zone diameters were 21.86 mm (75%), 19.64 mm (50%), and 18.37 mm (25%). The positive control (ciprofloxacin) measured 24.43 mm, while the negative control (DMSO) showed 0 mm. CONCLUSION: The 96% ethanol extract of lontar leaves demonstrated antibacterial activity against Staphylococcus aureus, with higher concentrations yielding stronger inhibition.
Co-Authors Asdar Tajuddin Rifqah Amaliah Rolly Riksanto Bahtiar