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All Journal Biology, Medicine, & Natural Product Chemistry Sonde (Sound of Dentistry)
Didi Nugroho Santosa
Universitas Trisakti
Biochemistry, Genetics & Molecular Biology Dentistry Medicine & Pharmacology Public Health

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Potensi Antibakteri dan Antibiofilm Kombinasi Sefadroksil dengan Ekstrak Etanol Daun Anredera cordifolia Terhadap Porphyromonas gingivalis Mulyani C; Natassya P; Didi Nugroho Santosa
SONDE (Sound of Dentistry) Vol. 10 No. 1 (2025): SONDE (Sound of Dentistry)
Publisher : Maranatha Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstrak Proses inflamasi kronis oleh bakteri penyebab periodontitis dapat berdampak serius jika tidak ditangani dengan tepat. Porphyromonas gingivalis merupakan bakteri utama yang berkontribusi pada periodontitis kronis. Terapi antibiotik sering digunakan untuk mengatasi penyakit periodontal, tetapi penggunaannya yang berlebihan dan tidak tepat dapat memicu resistensi antibiotik. Kombinasi antibiotik dengan ekstrak bahan alam menjadi alternatif untuk meningkatkan efektivitas antibiotik. Daun binahong (Anredera cordifolia) memiliki sifat antibakteri karena kandungan senyawa metabolit sekundernya, seperti flavonoid, saponin, dan triterpenoid. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dan antibiofilm kombinasi sefadroksil dan ekstrak etanol daun A.cordifolia terhadap P.gingivalis. Penelitian ini berupa eksperimental laboratoris secara in vitro dengan rancangan post-test-only control group design. Penelitian diawali dengan uji fitokimia dan uji MBC sefadroksil terhadap P.gingivalis. Lalu uji antibakteri dilakukan dengan metode plate count dan uji antibiofilm dilakukan dengan metode microtiter plate biofilm assay. Sampel yang digunakan adalah kombinasi sefadroksil 1,2 µg/ml dengan ekstrak etanol daun A.cordifolia 6,25%, 12,5%, 25%, 50%, dan 100%, sefadroksil 1,2 µg/ml, sefadroksil 2,4 µg/ml sebagai kontrol positif, dan akuades sebagai kontrol negatif. Hasil MBC sefadroksil terhadap P.gingivalis adalah 2,4 µg/ml. Berdasarkan penelitian diketahui, kombinasi sefadroksil dengan ekstrak etanol daun A.cordifolia menunjukkan aktivitas antibakteri terhadap P.gingivalis pada konsentrasi 12,5% hingga 100%. Sedangkan, aktivitas antibiofilm bekerja paling optimal diamati pada masa inkubasi 3 jam. Kesimpulan penelitian ini adalah kombinasi sefadroksil dengan ekstrak etanol daun A.cordifolia berpotensimenghambat pertumbuhan bakteri dan pembentukan biofilm P.gingivalis. Kata kunci: antibakteri, antibiofilm, ekstrak etanol daun Anredera cordifolia, Porphyromonas gingivalis, sefadroksil
The Effect of Nano Chitosan Xylotrupes Gideon on Fibroblast Proliferation and Collagen Deposition in the Oral Mucosa of Rattus norvegicus Komariah Komariah; Muhammad Orliando Roeslan; Rezky Anggraeni; Didi Nugroho Santosa; Aubrey Kanya Rennata
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.23-30

Abstract

Lacerations of the oral mucosa are a common clinical problem and require an efficient healing process. The wound healing process involves the haemostasis, inflammation, proliferation, and maturation phases. During the proliferation phase, fibroblasts play an important role in migration and proliferation to produce collagen, which connects the wound's edges and provides strength and stability to the scar tissue that forms. Chitosan from the horned beetle (Xylotruphes gideon) contains active compounds that are thought to accelerate the healing process by increasing the activity of inflammatory cells and fibroblast proliferation. Physical modification into nanoparticles facilitates penetration into the mucosal layer, thereby accelerating collagen production and re-epithelialization. This study aimed to determine the effect of X. gideon nanokitosan administration on increasing fibroblast proliferation and collagen formation in vivo. The study was divided into five groups: positive control, negative control, and nano chitosan treatment at 3000, 1500, and 750µg/mL. All groups of experimental animals were previously given an injury to the right cheek mucosa with a No. 12 scalpel blade, and nano chitosan was administered topically. The increase in fibroblasts and collagen deposits was determined by HE and Masson's Trichrome staining on days 3, 7, and 14, which were observed microscopically and using ImageJ. The results showed a significant increase in fibroblasts on day 3 and collagen fibre deposition on day 14 in all treatment groups. The group with 750 ppm nano chitosan showed the best results in accelerating wound healing regarding fibroblast proliferation and collagen deposition. Conclusion: Nano chitosan X. gideon at a concentration of 750 ppm effectively accelerates wound healing in the cheek mucosa of mice by increasing fibroblast proliferation and collagen deposition.
Co-Authors Aubrey Kanya Rennata Komariah Komariah Muhammad Orliando Roeslan Mulyani C Natassya P Rezky Anggraeni