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All Journal Biology, Medicine, & Natural Product Chemistry Indonesian Journal of Health Research Innovation
I Putu Bayu Agus Saputra
Universitas Pendidikan Ganesha
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Fragrant Pandan (Pandanus ammaryllifolius) Leaves Ethanol Extract as an Enhancer of Endogenous Antioxidant Defence under High-Fat Diet Conditions Ni Made Wiasty Sukanty; Laksmi Nur Fajriani; I Putu Bayu Agus Saputra; Iptan Ariki; I Ketut Agus Yura Aditya
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1171-1176

Abstract

Excessive fat consumption, especially saturated and trans fatty acids, plays a significant role in metabolic disorders such as obesity, dyslipidemia, and cardiovascular disease through increased oxidative stress. Malondialdehyde (MDA), the end product of lipid peroxidation, serves as a biomarker of oxidative damage, whereas indirect bilirubin acts as an endogenous antioxidant. This study aimed to evaluate the effect of fragrant pandan leaves ethanol extract (FPLEE) in reducing MDA levels and modulating indirect bilirubin activity in rats fed a high-fat diet (HFD). The experimental method used 24 male Rattus norvegicus divided into six treatment groups: normal control (NC), positive control (C+), negative control (C-), and three FPLEE treatment groups with different doses (8, 16, and 32 mg/200 g BW/day). Administration was carried out for 14 days after 14 days of HFD induction. MDA levels were measured using the ELISA method, while total bilirubin and direct bilirubin were measured using spectrophotometry to determine indirect bilirubin levels. Statistical analysis was performed using one-way ANOVA and the post hoc Bonferroni test. The results showed that there were no significant differences in MDA levels between groups, although the C+ group fed a HFD showed a tendency toward increased MDA. Indirect bilirubin levels increased significantly in the low-dose FPLEE group (8 mg/200 g BW/day), indicating increased endogenous antioxidant activity, while higher doses did not. Thus, FPLEE has the potential to be an effective natural phytopharmaceutical agent in enhancing the body's antioxidant defence against oxidative stress caused by excessive fat consumption, especially at low doses.
PERAN NRF2 DAN NF-ΚB DALAM PATOGENESIS DIABETES MELITUS: NARRATIVE REVIEW I Putu Bayu Agus Saputra; Ni Made Wiasty Sukanty; Suci Fitriana Pramudya; Putu Kermawan; Irna Olvaliani Aimang
Indonesian Journal of Health Research Innovation Vol. 3 No. 2 (2026): Indonesian Journal of Health Research Innovation
Publisher : Yayasan Menawan Cerdas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64094/7hf4mg65

Abstract

Diabetes melitus tipe 2 (DMT2) merupakan gangguan metabolik kronis dengan prevalensi global yang meningkat pesat, yang ditandai oleh hiperglikemia persisten akibat resistensi insulin dan disfungsi sel beta pankreas. Pada tingkat molekuler, patogenesisnya berkaitan erat dengan stres oksidatif dan inflamasi kronis yang dimediasi oleh interaksi antara jalur Nuclear factor erythroid 2-related factor 2 (Nrf2) dan Nuclear Factor Kappa B (NF-κB). Penelitian ini bertujuan untuk menganalisis secara komprehensif hubungan silang (crosstalk) antara kedua jalur tersebut melalui tinjauan naratif dengan pencarian literatur terstruktur dari PubMed, ScienceDirect, dan Google Scholar. Hasil kajian menunjukkan bahwa hiperglikemia kronis meningkatkan produksi Reactive Oxygen Species (ROS), yang memicu aktivasi NF-κB serta meningkatkan ekspresi sitokin proinflamasi seperti TNF-α dan IL-6, yang berkontribusi terhadap resistensi insulin dan kerusakan pankreas. Sebaliknya, Nrf2 berperan sebagai regulator antioksidan utama dengan menginduksi enzim seperti glutathione peroxidase; namun, aktivitasnya menurun pada kondisi stres oksidatif yang berkepanjangan. Ketidakseimbangan antara hiperaktivasi NF-κB dan disfungsi Nrf2 ini mempercepat progresivitas penyakit. Sebagai kesimpulan, penargetan keseimbangan antara kedua jalur tersebut merupakan strategi terapeutik yang menjanjikan untuk mencegah komplikasi dan meningkatkan luaran metabolik pada DMT2.
Co-Authors I Ketut Agus Yura Aditya Iptan Ariki Irna Olvaliani Aimang Laksmi Nur Fajriani Ni Made Wiasty Sukanty Putu Kermawan Suci Fitriana Pramudya