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Effect of Essential Oil of Tejpat, Black Pepper, and Cardamom as a Natural Food Preservatives for Sweet Orange (Citrus sinensis) Juice Sagar Mani Paudel; Bishal Thagunna; Deepa Khadka; Rashmi Baral; Deepa Thapa
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1137-1141

Abstract

Essential oil of tejpat (Cinnamomum tamala), black pepper (Piper nigrum) and cardamom (Amomum subulatum roxb.) collected by hydro distillation, and were tested as natural food preservative in orange juice (Citrus sinensis). The physiochemical changes such as pH, TSS, acidity, total microbial count, yeast and mold count were evaluated at fix time intervals of 4 hours. for 24 hours. It has been observed that the juice sample infused with black pepper essential oil demonstrated superior preservation of TSS, pH and acidity compared to the other variants. Orange juice supplemented with essential oils maintained the microbial count and yeast and mold count below 100 CFU/ml for up to 24 hours. After 24 hours of storage, all samples surpassed the total microbial count. Among the essential oils, black pepper essential oil demonstrated superior preservation compared to the others.
Effect of Repeated Frying of Potato Chips on Physiochemical Properties of Different Frying Oils and Its Sensory Evaluation Rabin Poudel; Bishal Thagunna; Bishal Bhattrai; Sushan Subedi; Nishal Baral
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1599-1605

Abstract

Deep frying involves submerging food in heated oil at about 180°C. Throughout the deep-frying process, various chemical reactions occur, leading to alterations in the physicochemical characteristics of the oil, which can ultimately have detrimental health impacts on consumers. Nevertheless, for reasons of economic efficiency, both home and industrial users frequently reuse cooking oils for deep frying. Consequently, this research aimed to evaluate the physicochemical and nutritional properties of commercially available mustard oil, sunflower oil, soybean oil, and palm oil, in addition to investigating how the repeated deep frying of potato tubers affects the physicochemical and nutritional qualities of the analyzed oil samples. Consequently, the ideal amount of frying cycles for every type of oil was explored. To accomplish this, potato was fried individually using coconut, palm, and sunflower oils over four successive frying cycles. Results revealed that increasing the frying counts resulted in increased peroxide value, acid value, viscosity and refractive index, whereas decrease in moisture, iodine value and DPPH value of four oil samples. The peroxide value (PV) and acid value of mustard oil, sunflower oil, soybean oil, and palm oil showed increases ranging from 1.82 to 7.98 meq/Kg, 5.19 to 11.69 meq/kg, 4.81 to 9.89 meq/kg, and 2.49 to 11.08 meq/kg respectively, with acid values shifting from 1.24 to 6.74 mg KOH/g, 0.17 to 0.49 mg KOH/g, 0.25 to 0.42 mg KOH/g, and 0.80 to 2.86 mg KOH/g. The viscosity increased from 183.33 to 615.09 mPA s, 51.66 to 405.49 mPA s, 165.57 to 590.17 mPA s, and 376.42 to 711.43 mPA s of mustard oil, sunflower oil, soybean and palm oil respectively, while the refractive index increased from 1.4654 to 1.4672, 1.4668 to 1.4710, 1.467 to 1.4722, and 1.4552 to 1.4610 of mustard, sunflower, soybean and palm oil respectively. In contrast, there was a reduction in moisture content, iodine value, and DPPH value for mustard, sunflower, soybean, and palm oil, ranging from 0.16-0.06%, 0.09-0.03%, 0.11-0.04%, and 0.21-0.09%, respectively. The findings indicate that the iodine values decreased from 106.2-88.6 g, 126.2-106.5 g, 129.4-108.4 g, and 54.3-31.82 g, respectively. The DPPH values of oil samples were 63.25%, 72.08%, 82.55%, and 78.18%, respectively. Following the fourth frying cycle, these values dropped to 47.85%, 53.79%, 79.62%, and 69.58%. Higher sensory scores regarding overall acceptability were obtained by chips fried in palm oil and lower by mustard oil.
Optimization of Calcium- Enriched Cookies Incorporating Eggshell and Shiitake Powder Roshan Kunwar; Bishal Thagunna; Smriti Paudel; Anjana Lamichhane; Apekshya Bhattarai; Sandhya Subedi
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1021-1025

Abstract

Eggshells from chickens can serve as a source of dietary calcium. This research examined the impact of incorporating eggshell powder on the proximate, mineral, and sensory characteristics of cookies. The cookies were made using wheat flour, enhanced with eggshell powder in proportions of 3, 5, 7, and 9%, along with shitake mushroom powder in amounts of 3, 4, 5, and 6%. The samples were labeled as A, B, C, D and E. The dough was prepared using a multi-stage mixing technique, like the creaming method. After that, it was shaped into round cookies, which were baked in a preheated oven at 180 oC for 25 minutes. The results obtained showed that incorporating eggshell powder significantly raised the calcium levels in the cookies, measuring 1085.05, 1505.20, 1975.22, and 2450.11 mg/100g at concentrations of 3%, 5%, 7%, and 9%, respectively. Additionally, there is a slight alteration in the texture and sensory characteristics of the cookies. The iron content of cookies containing shitake mushroom powder at 3, 4, 5, and 6 % were 3.50, 3.41, 3.36, and 2.28 mg/100g respectively. The proximate analysis revealed that adding eggshell and shiitake mushroom powder increased the protein content from 8.83% to 14.67%, the ash content from 1.56% to 4.59%, and the fiber content from 0.92% to 2.88%. Simultaneously, it reduced the moisture content from 5.78% to 4.69% and the fat content from 19.03% to 15.93%. To summarize, adding eggshell powder and shiitake mushroom could improve the nutritional profile of traditional cookies, particularly in terms of calcium, ash, fiber, and protein levels.
Formulation and Assessment of Nutritious Complementary Food Blended with Germinated (Maize, Millet and Soybean), Carrot, and Coconut Flour Season Khanal; Bishal Thagunna; Sonika Ghimire; Ishori Bhandari; Binod Sunar
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1065-1069

Abstract

In developing nations, malnutrition related to protein and energy in young children is possibly the most widespread health issue. Complementary foods are essential for the growth and development of children as they address both the nutritional and developmental requirements of infants when breastfeeding alone is insufficient. The purpose of this study was to evaluate effects of blending ratio and processing technique on physicochemical composition, and sensory acceptability of quality germinated maize, millet, and soybean, along with carrot and coconut flour based complementary food. The different staples were processed into flours and were combined in ratios of (70:10:10: 5:5), (60:15:15:5:5), (50:20:20:5:5), and (40:25:25:5:5) of maize flour, millet flour, soybean flour, carrot and coconut flour and the samples were denoted as A, B, C, and D. The result revealed that composite the moisture levels varied from 6.55% to 7.15%, with sample C containing 50% maize flour exhibiting the highest moisture content, while sample D with 40% maize flour showed the lowest moisture content. The protein content values of all samples exceeded the 15%. The fat content of the complementary food samples varied between 6.44% and 8.58%, showing highest in sample highest in sample A and lowest in sample E. The range of crude fiber content varied from 1.89% to 4.73%, with sample D showing the highest level and sample E the lowest. The ash content of the formulated complementary food samples increased with the rising addition of maize, soybean, and millet flour. From the sensory analysis sample C recorded the highest score.