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Study of Microorganisms Associated with Spoilage of Onions Sold in Mbiama Market, Rivers State, South South Nigeria Imarenezor Edobor Peter Kenneth; Opara Christiana Ngozi; Efere Yarwadum Samson
African Journal of Sciences and Traditional Medicine Vol 3 No 1 (2026): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v3i1.8802

Abstract

Onions are a staple vegetable in many Nigerian households, and their spoilage can contribute to significant economic losses and food insecurity. This study investigated the microorganisms associated with the spoilage of onions sold in Mbiama Tuesday Market, Rivers State, South-South Nigeria, and examined their potential implications for public health. A total of 100 onion samples were collected, and the associated microorganisms were isolated, identified, and characterized using standard bacteriological and mycological techniques, including biochemical testing and antibiotic sensitivity assays. The results showed that the onions were contaminated with a variety of bacteria—Bacillus spp., Pseudomonas spp., Staphylococcus spp., and Escherichia coli—and fungi, including Mucor, Aspergillus niger, yeast, and Penicillium spp., all of which were capable of causing soft rot, discoloration, and decay. Among the bacterial isolates, E. coli was the most frequent (23.5%), whereas Pseudomonas spp. was the least frequent (9.8%); among the fungi, A. niger was the most frequent (13.7%) and Mucor the least frequent (5.9%). Bacterial colony counts ranged from 5.2 × 10⁵ CFU/g (E. coli) to 5.6 × 10⁵ CFU/g (Pseudomonas spp.), while fungal counts ranged from 1.4 × 10⁵ CFU/g (yeast) to 1.58 × 10⁵ CFU/g (A. niger). Biochemical tests revealed that Bacillus spp. and Staphylococcus spp. were catalase and coagulase positive, whereas Pseudomonas spp. and E. coli were coagulase negative. Antibiotic sensitivity testing showed that Staphylococcus spp. was resistant to the tested antibiotics, while Bacillus spp., E. coli, and Pseudomonas spp. remained sensitive. The study concludes that onions sold in Mbiama Market are contaminated with diverse spoilage-causing bacteria and fungi, posing a potential risk to public health. These findings underscore the need for proper handling, storage, and preservation practices, such as maintaining cool, dry storage conditions and ensuring hand hygiene among vendors and consumers as well as further research to develop effective strategies for controlling microbial spoilage of onions in Nigeria.
Determination of Antagonistic Effects of Sludge and Urine-Associated Bacteria against Staphylococcus aureus and Escherichia coli Imarenezor Edobor Peter Kenneth; Opara Christiana Ngozi; Efere Yarwadum Samson
African Journal of Clinical Medicine and Pharmacy Research Vol 3 No 1 (2026): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajcmpr.v3i1.8284

Abstract

Abattoirs, due to their very many activities, is a source of microbial diversity, many of which are able to prevent the growth of other closely related bacteria. Hence, this work was carried out to isolate and identify bacteria from sludge and urine, and evaluate their antagonistic effects against Staphylococcus aureus and Escherichia coli. The antagonistic effects of bacterial pathogens from abattoir sludge against Staphylococcus aureus and Escherichia coli. The culture and identification of bacteria from abattoir sludge was done using standard microbiological techniques and the bacterial antagonism assay was done using the overlay method. From the abattoir sludge, Bacillus species (37.5%) was the most prevalent, followed by Shigella species (12.5%), Escherichia coli (12.5%), Staphylococcus aureus (12.5%), Proteus species (12.5%), and Klebsieilla. species (12.5%). The bacterial isolates were most resistant to Ciprofloxacin, Cefotaxime, Cefuroxime, Imipenem and most sensitive to Nitrofurantoin, Nalixidic Acid, Gentamicin, Erythromycin, Ofloxacin, AZN. From the study, none of these abattoir sludge-associated bacteria showed antagonistic effect against Staphylococcus aureus and against Escherichia coli. This study demonstrates the antagonistic potential of sludge and urine-associated bacteria against Staphylococcus aureus and Escherichia coli. The identified isolates, particularly Pseudomonas aeruginosa and Bacillus subtilis, exhibited significant inhibitory activity, suggesting their potential as sources of novel antimicrobial compounds. These findings highlight the importance of exploring environmental and human-associated microbiota for bioactive molecules that could address the growing challenge of antibiotic resistance.
Diarrhoea among Children under Five Years Old in Relation to Household Safe Storage Practices in Wukari Local Government Area, Taraba State, Nigeria Imarenezor Edobor Peter Kenneth; Boyi Hassan; Opara Christiana Ngozi; Efere Yarwadum Samson
African Journal of Medicine, Surgery and Public Health Research Vol 3 No 1 (2026): African Journal of Medicine, Surgery and Public Health Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajmsphr.v3i1.8285

Abstract

Diarrhoea remains a leading cause of morbidity and mortality among under‑five children in Nigeria, particularly in rural settings where unsafe water handling and inadequate storage prevail. Diarrhea is defined as the passing of three or more watery or loose stools per day, or more frequent passage than normal for an individual. This cross‑sectional study assessed the determinants of diarrhoea in relation to household water practices in Wukari Local Government Area (LGA), Taraba State. The aim was to identify water‑related risk factors—source type, treatment method, storage container, and hygiene behaviours—that influence diarrhoeal occurrence among children <5 years. A community‑based survey was conducted across randomly selected areas of Wukari LGA. Structured questionnaires captured socio‑demographics, water source (protected well, borehole, surface, sachet), treatment (boiling, chlorination, filtration, none), storage (covered narrow‑necked jerry can, open bucket, other), and hand‑washing practices. A total number of 200 sample were collected for this study among children of five (5) years of age. The result shows a prevalence of 34% (n=200) representing 34% (68) respondents of positive and 66% representing 132 respondents of negative. In conclusion, this study demonstrates that despite relatively high access to improved water sources (protected wells/boreholes) in Wukari, the prevalence of diarrhoea among children under five remains elevated (34%) because of pervasive unsafe practices downstream—principally failure to treat water before consumption, storage in uncovered buckets that permit re‑contamination, and fetching water with unclean hands. Microbiological evidence confirmed significantly higher faecal coliform counts in open‑bucket water, substantiating the causal pathway. Maternal education acted as a buffer, underscoring the role of health literacy. The findings indicate that interventions must shift focus from mere source improvement to promoting point‑of‑use safety—treatment, covered storage, and hand hygiene—to achieve meaningful reductions in diarrhoeal disease. To this end, it is recommended that; Community‑based water safety education, provision of affordable safe‑storage vessels, point‑of‑use water treatment promotion, strengthen maternal health literacy, microbial monitoring, policy advocacy and research follow‑up. Implementation and follow‑up practices which has to do with giving priorities to areas with highest diarrhoea prevalence (identified in survey) for rapid rollout; monitor via existing Integrated Disease Surveillance & Response (IDSR) system will reduce the prevalent rate of under-five diarrrhoea in Wukari which by extension Taraba State and North East in general.