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All Journal Hearty : Jurnal Kesehatan Masyarakat Journal of Agriculture and Animal Science
Tania Anggreani Wijaya
Universitas Airlangga
Agriculture, Biological Sciences & Forestry Other

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EFFECT OF RUELLIA TUBEROSA L. LEAF NANOPARTICLES ON SEMINIFEROUS TUBULE DIAMETER AND GERMINAL EPITHELIUM THICKNESS IN DIABETIC RATS Tania Anggreani Wijaya; Daffa Alice Pavita; Yunia Chrismonica
HEARTY Vol 14 No 2 (2026): APRIL
Publisher : Fakultas Ilmu Kesehatan, Universitas Ibn Khaldun, Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/hearty.v14i2.22079

Abstract

Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia that can cause various pathological changes in the human body and animals. Oxidative stress due to diabetes can lead to spermatogenesis disorders and increased cell apoptosis in the testes. Ruellia tuberosa L. leaf contain antioxidant compounds, such as saponins, carotenoids, flavonoids, and phenols, that can be used as free radical scavengers to reduce oxidative stress levels. The manufacture of Ruellia tuberosa L. leaf nanoparticles using the top-down method enhances pharmacological activity, particularly by facilitating cell wall penetration and improving the absorption of active substances in the body. This study employed a proper experimental design with a post-test-only control group model using Wistar rats as experimental animals. The treatment groups in this study included K- (control), K+ (diabetic control), P1 (exogenous long-acting insulin 1.80 IU/kg BW), P2 (Ruellia tuberosa L. leaf nanoparticles dose of 200 mg/kg BW), and P3 (Ruellia tuberosa L. leaf nanoparticles dose of 400 mg/kg BW). Testicular retrieval was performed 28 days after administration of Ruellia tuberosa L. leaf nanoparticles. Testicular histopathology was stained using Hematoxylin-Eosin staining and examined under a trinocular microscope at 100X magnification. Data on the diameter and thickness of the germinal epithelium were analyzed using the ANOVA test, followed by Duncan’s test. The results showed that administration of Ruellia tuberosa L. leaf nanoparticles at a dose of 400 mg/kg BW was an effective dose in increasing the diameter of the seminiferous tubules and the thickness of the germinal epithelium compared to Ruellia tuberosa L. leaf nanoparticles at a dose of 200 mg/kg BW and exogenous long-acting insulin 1.80 IU/kg BW
Prevalensi dan Identifikasi Nematoda Gastrointestinal pada Sapi Potong di Rumah Potong Hewan Majeluk, Kota Mataram Muhammad Ulul Azmi; Tania Anggreani Wijaya; Imam Mustofa; Sri Mulyati; Erma Safitri
AgriMalS Vol 6 No 1 (2026): Volume 6 Nomor 1 Tahun 2026
Publisher : Universitas Muhammadiyah Kotabumi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47637/agrimals.v6i1.2241

Abstract

Nematodiasis is a parasitic disease caused by infection with worms belonging to the class Nematoda and remains one of the major constraints in the livestock sector in Indonesia due to its impact on animal health and productivity. This study aimed to determine the prevalence of gastrointestinal nematode eggs in beef cattle slaughtered at the Majeluk Slaughterhouse, Mataram City. A descriptive study was conducted using 96 fecal samples collected over an eight-week period. Samples were obtained directly from the rectum of cattle immediately after slaughter. Laboratory examinations were performed using native smear and flotation techniques to identify nematode eggs based on their morphological characteristics under microscopic observation. Prevalence was calculated as the proportion of positive samples relative to the total number of samples examined. Of the 96 samples analyzed, 36 were positive for gastrointestinal nematode eggs, resulting in a prevalence rate of 37.5%. Four types of nematode eggs were identified: Trichostrongylus sp., Oesophagostomum sp., Toxocara vitulorum, and Ostertagia sp. These findings indicate that gastrointestinal nematode infections are still present in slaughtered cattle at a moderate prevalence level. Therefore, improved control measures, including regular anthelmintic treatment and better livestock management practices, are necessary to reduce infection rates and minimize potential economic losses.
Co-Authors Daffa Alice Pavita Erma Safitri Imam Mustofa Muhammad Ulul Azmi Sri Mulyati Yunia Chrismonica