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Debby Dijola Moniharapon
Department of Biology, Faculty of Science and Technology, Pattimura University, Ambon - Indonesia
Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology Medicine & Pharmacology

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Efficacy Test of Ethanol Extract From Matoa (Pometia pinnata) Stem Bark on Aedes aegypti Larval Mortality As An Effort to Control Dengue Fever Debby Dijola Moniharapon; Mechiavel Moniharapon
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp1-9

Abstract

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus and transmitted primarily through the bite of the Aedes aegypti mosquito, the disease's primary vector. Controlling the population of this mosquito species is considered a strategic measure in preventing the spread of DHF. One potential biological control agent is the matoa plant (Pometia pinnata), a member of the Sapindaceae family widely distributed in tropical regions. This plant contains secondary metabolites such as flavonoids, tannins, and saponins, which exhibit natural insecticidal activity. This study aims to evaluate the efficacy of ethanol extract derived from the bark of P. pinnata against A. aegypti larvae. The extraction process was conducted using ethanol as a solvent, and the resulting extract was tested against mosquito larvae at concentrations of 25 ppm, 50 ppm, 70 ppm, and 90 ppm. Larval mortality data were analysed using Analysis of Variance (ANOVA) via SPSS software version 24.00. The results demonstrated that the ethanol extract of P. pinnata bark had a significant dose-dependent effect on larval mortality. These findings suggest the potential of the extract as a natural active ingredient in the formulation of plant-based larvicides, contributing to environmentally friendly and sustainable dengue vector control strategies.
Utilization of Lime Leaf (Citrus aurantilofia) Extract as an Eco-Friendly Alternative Strategy for the Management of Sitophilus zeamays Debby Dijola Moniharapon; Evelin Tuhumuri; Mechiavel Moniharapon; Cindri Tasane
Biofaal Journal Vol 7 No 1 (2026): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v7i1pp78-89

Abstract

The maize weevil (Sitophilus zeamays) is a major postharvest pest that frequently damages stored grains, as evidenced by characteristic feeding perforations. The use of botanical insecticides represents an environmentally friendly alternative for pest management. One promising natural resource is lime leaf (Citrus aurantifolia). This study aimed to evaluate the effectiveness of lime leaf extract on the mortality of the maize weevil (Sitophilus zeamays). A laboratory-based experimental approach was employed using five dosage treatments (0 g as the control, 3 g, 8 g, 13 g, and 18 g), each replicated three times. Observations were conducted at 0, 12, 14, 16, and 18 hours after exposure. Data were analyzed using a two-way analysis of variance (two-way ANOVA) to simultaneously assess the effects of dosage and exposure time on pest mortality. The results indicated that lime leaf extract was effective as a botanical insecticide, with mortality rates increasing in proportion to both dosage and duration of exposure. The most effective treatment was 18 g, which resulted in the highest mortality rate of 98.34% after 18 hours. These findings suggest that lime leaf extract has strong potential for development as a botanical insecticide for sustainable and environmentally safe storage pest management.
Co-Authors Cindri Tasane Evelin Tuhumuri Mechiavel Moniharapon