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Mukarram Mudjahid
Department of Pharmacy, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia
Medicine & Pharmacology Public Health

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Evaluating Curcumin Intake on Metabolism-Related Genes in Drosophila melanogaster Jonathan Elbert Karsten Halim; Asbah Asbah; Nadila Pratiwi Latada; Mukarram Mudjahid; Usmar Usmar; Risfah Yulianti; Firzan Nainu
International Journal of Biomedical Science and Travel Medicine 24-29
Publisher : Publication Department, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/ijbstm.1.1.2024.24-29

Abstract

Background Aging entails a gradual deterioration of physiological functions within the body. Current research provides evidence suggesting that curcumin may extend the lifespan of fruit flies by mitigating the effects of aging. However, the precise concentration of curcumin necessary to induce favorable phenotypic and molecular outcomes in fruit flies has yet to be determined. Method The study utilized the capillary feeder (CAFE) assay on male Oregon-R flies, and examined the expression of the srl and pepck genes through the reverse transcriptase quantitative PCR (RT-qPCR) method. Case An elevation of curcumin consumption was examined in the treatment groups that were provided with feed containing curcumin concentrations of 50 μM and 250 μM. This observation is consistent with the increased lifespan noted in the Drosophila groups consuming higher concentrations of curcumin. Meanwhile, molecular analysis at the expression level of the srl and pepck genes revealed no significant change in gene expression between the treatment and control groups. Conclusion The use of the CAFE assay assists researchers in quantitatively measuring the amount of curcumin intake in Drosophila melanogaster. However, the consumption of curcumin in this experiment did not demonstrate a significant impact on the metabolism-related genes of Drosophila, srl and pepck genes.
Impact of Ethanol Exposure on Survival and the Expression of Endogenous Antioxidants in Drosophila melanogaster L.M Alif Fauzan Tamar; Nur Islamiah Syahrir; Khansa Khansa; Reski Amalia Rosa; Nadila Pratiwi Latada; Sri Wahyuni M; Nur Rahma Rumata; Risfah Yulianty; Mukarram Mudjahid; Firzan Nainu
International Journal of Biomedical Science and Travel Medicine 56-60
Publisher : Publication Department, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/ijbstm.1.2.2024.56-60

Abstract

Background Ethanol, a widely consumed psychoactive substance, disrupts cellular and molecular processes, leading to a range of adverse physiological effects. Prolonged exposure to ethanol has been associated with severe neurological disorders, underscoring the importance of investigating its toxicological impact. This study aims to evaluate the effects of ethanol exposure on the phenotypical characteristics of Drosophila melanogaster, with a particular focus on survival and the expression of endogenous antioxidant-related genes. Methods The study utilized an ethanol exposure assay on male w1118 flies, assessing survival and the expression of the sod1, sod2, and cat genes through reverse transcriptase quantitative PCR (RT-qPCR). Results Ethanol exposure negatively affected survival in a concentration-dependent manner, with lower survival rates observed in Drosophila groups exposed to higher ethanol concentrations. Additionally, molecular analysis highlighted the significant role of the endogenous antioxidant gene cat in promoting survival in Drosophila under ethanol exposure. Conclusion The findings of this study indicate that ethanol exposure is lethal to D. melanogaster in a concentration-dependent manner, with catalase playing a key role in mitigating its toxic effects and enhancing fly survival.
Phenotypical analysis of Chloramphenicol toxicity in Drosophila Aditya Satya Pratama; Aizia Risty Rizal; Nurhidayah Ramly; Gimas Fatir Bijaksana; Jihan Atiqah Permatasari; Muhammad Akbar Bahar; Nadila Pratiwi Latada; Mukarram Mudjahid; Risfah Yulianty; Nur Inda Yanti; Firzan Nainu
International Journal of Biomedical Science and Travel Medicine 19-26
Publisher : Publication Department, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/ijbstm.2.1.2025.19-26

Abstract

Background: Chloramphenicol is a broad-spectrum antibiotic with serious side effects, including aplastic anemia and gray baby syndrome. While Drosophila melanogaster is a cost-effective and genetically relevant model for toxicological studies, its response to chloramphenicol remains unexamined. This study explores the toxic effects of chloramphenicol in Drosophila to provide insights into its broader biological impact. Methods: This study aims to analyze the toxicity of chloramphenicol in terms of developmental toxicity, locomotor activity, morphology and gene expression status (sod1, sod2, tom40, and indy) in Drosophila melanogaster. The study was conducted on Oregon-R strain D. melanogaster larvae fed with chloramphenicol at concentrations of 625; 1,875; 3,125; and 4,375 ppm. Results: Developmental toxicity assay revealed that chloramphenicol exposure significantly delayed developmental progression, as evidenced by a prolonged transition from larval to pupal stages at concentrations of 3,125 and 4,375 ppm. However, no significant alterations were observed in locomotor activity or morphological characteristics. Moreover, chloramphenicol exposure in D. melanogaster appeared to exert toxic effects by significantly altering the expression of sod1, sod2, and tom40, while having no detectable impact on indy gene expression. Conclusion: High concentrations of chloramphenicol significantly impair larval development in D. melanogaster and alter gene expression profiles. However, adult flies exhibit no observable morphological or locomotor abnormalities compared to controls. These findings highlight that Drosophila larvae are susceptible to chloramphenicol toxicity, making it an ideal phase for assessing the detrimental effects of chloramphenicol.
Co-Authors Aditya Satya Pratama Aizia Risty Rizal Asbah Asbah Firzan Nainu Gimas Fatir Bijaksana Jihan Atiqah Permatasari Jonathan Elbert Karsten Halim Khansa Khansa L.M Alif Fauzan Tamar Muhammad Akbar Bahar Nadila Pratiwi Latada Nadila Pratiwi Latada Nur Inda Yanti Nur Islamiah Syahrir Nur Rahma Rumata Nurhidayah Ramly Reski Amalia Rosa Risfah Yulianti Risfah Yulianty Sri Wahyuni M Usmar Usmar