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All Journal JSMARTech : Journal of Smart Bioprospecting and Technology
Wulandari, Dian Novita Wulandari
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Phytochemical Screening and Antioxidant Activity Testing of Young and Old Bidara Fruit Using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Method Pratiwi, Lidia Wahyu Pratiwi; Jayanti, Gatra Ervi Jayanti; Faisal, Faisal; Wulandari, Dian Novita Wulandari; Widyaningrum, Ike Widyaningrum
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 7 No. 1 (2026): Vol. 7 No. 1 (2026): JSMARTech Volume 7, No 1, 2026
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2026.007.02.08

Abstract

This study evaluated the phytochemical constituents and antioxidant activity of Ziziphus mauritiana fruit at young and old ripening stages. Ethanol extracts were subjected to qualitative phytochemical screening and antioxidant activity assessment using the DPPH assay. Both young and old fruit extracts tested positive for alkaloids but lacked detectable flavonoids, tannins, saponins, and triterpenoids. Antioxidant activity was weak in both extracts, with IC₅₀ values of 3882.44 ppm (young) and 4391.79 ppm (old). Statistical analysis showed no significant difference in antioxidant activity between ripening stages (p > 0.05). Young fruit displayed slightly higher inhibition at increasing concentrations. These results suggest that the ripening stage does not significantly affect antioxidant activity in Z. mauritiana fruit. Further research with alternative extraction methods and assays is recommended.
Co-Authors Faisal Faisal Jayanti, Gatra Ervi Jayanti Pratiwi, Lidia Wahyu Pratiwi Widyaningrum, Ike Widyaningrum