Jurnal Media Laboran
Vol 6 No 1 (2016): Mei

Pengaruh Penambahan Ekstrak Khamir Terhadap Aktivitas Antibakteri Isolat Fungi Endofit Genus Paecilomyces Dari Kulit Buah Kakao (Theobroma Cacao L)

Asnurbaety Dwiyana (Universitas Indonesia Timur)



Article Info

Publish Date
18 May 2016

Abstract

A research about the effect of yeast extract addition on the antibacterial activity of endophytic fungi Paecilomyces genus from cacao pod husk (Theobroma cacao L.) has been conducted. The purpose of this research vas to know the effect of adding yeast extract on the antibacterial activity produced by endophytic fungi of cacao pod husk. Isolate of endophytic fungi fermented in Potato Dextrose Broth (PDB) medium with the addition of yeast extract 0.25% w/v, 0.5% w/v , 1 % w/v, 2% w/v, on a rotary shaker at 150 rpm for 7 days and then centrifuged at 3000 rpm for 15 minutes. The antibacterial activity of supematant was determined by measuring the growth inhibition of pathogenic bacteria Streptococcus mutans with agar diffusion method using paper disc for 24 hours incubation. The inhibition zone of negative control and sample with the addition of yeast extract 0.25% w/v, 0.5% w/v , 1% w/v 2% w/v to S mutans were 7.2 mm, 7.3 mm. 7.7 mm, 8.0 mm, and 8.4 mm respectively. These results indicated that yeast extract addition 1% w/v and 2% w/v have the most significantly influence toward antibacterial activity effect of endophytic fungi Paecilomyces genus.

Copyrights © 2016






Journal Info

Abbrev

MedLAb

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Public Health Other

Description

JURNAL MEDIA LABORAN merupakan Jurnal ilmiah yang dikelolah oleh Prodi D3 Analis Kesehatan Fakultas Kesehatan Masyarakat Universitas Indonesia Timur Makassar. Jurnal yang terbit dua kali setahun ini, yakni di bulan Mei dan november memuat artikel-artikel yang lebih komprehensif di bidang ...