Tofu industry is one of the industries which causing pollution on the environment especially water environment. The amount of wastewater-dumped and the content of organic substances could damage the water ecosystem. Multi Soil Layering (MSL) as one of alternative method is used in order to remove the tofu industrial wastewater pollution. MSL is a method of wastewater treatment that enhances the function of soils to purify wastewater. The research aimed to determine optimum loading rate and efficiency of MSL system to remove TSS, BOD and COD. This research was carried out in February, 2019. The results revealed efficiency of MSL system to remove TSS, BOD and COD were 86,86% ; 78,05% ; and 89,75 % respectively.
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