Akta Agrosia
Vol 18 No 2 (2015)

Identifikasi Genetik Kultivar Padi Gogo dengan Menggunakan Marka RAPD

Simarmata, Marulak (Unknown)
Rustikawati, Rustikawati (Unknown)



Article Info

Publish Date
01 Dec 2015

Abstract

A research was conducted to identify the genetics of upland rice cultivars using random amplified polimorphic DNA (RAPD) markers. Five out of 60 random primers that capable to amplify the most number of markers were OPE-07 (AGATGCAGCC), OPE-15 (ACGCACAACG), OPH-13 (GACGCCACAC), OPH19 (CTGACCAGCC), and OPM-02 (ACAACGCCTC). The number of markers amplified were 5, 6, 8, 8, and 7, respectively. RAPD-PCR using these 5 primers arranged to DNA templates of 41 rice cultivars amplified 1127 RAPD markers. Polymorphic information content (PIC) of each primer were 0.79, 0.80, 0.87, 0.87, and 0.72, respectively. Cluster analysis using a UPGMA dendogram showed that the 41 cultivars were grouped into 9 clusters with genetic similarity index more than 90 percent.

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Journal Info

Abbrev

Agrosia

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Akta Agrosia is dedicated to researchers or academics who intent on publishing research, scientific thinking, or other scientific ideas. The articles published in the Akta Agrosia are the author’s original works covering on Crop Production, Crop Physiology, Pest and Disease, Soil Science, ...