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All Journal AGRIVITA, Journal of Agricultural Science Agrin : Jurnal Penelitian Pertanian AGRITROPICA : Journal of Agricultural Sciences Akta Agrosia JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Pada Masyarakat
Rustikawati Rustikawati
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EVALUATION OF GROWTH AND YIELD PERFORMANCE ON INOCULATED CHILI PEPPER HYBRIDS BY CUCUMBER MOSAIC VIRUS Herison, Catur; Handayaningsih, Merakati; Fahrurrozi, Fahrurrozi; Rustikawati, Rustikawati
AGRIVITA, Journal of Agricultural Science Vol 36, No 1 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tolerance cultivar is a best control measure to reduce losses due to virus attack in the field.  Study on growth and yield of new cultivars compared to the commercial ones is an important step in cultivar development whether they will be accepted by farmers or not. The objective of this research was to study growth and yield of new chili pepper hybrids developed for CMV tolerance in comparison to a commercial and similar fruit size one. Twelve new hybrids and one commercial hybrid cultivar Prada, as a control, were evaluated in a completely randomized design with 3 replications. In the greenhouse experiment, H23 and H20 showed better vegetative growth compared to other hybrids and control. Hybrid H17 showed the highest yield followed by H5, H4 and H6.  Keywords.  growth, yield, chili pepper hybrids, CMV tolerance
IDENTIFICATION OF M4 GAMMA IRRADIATED MAIZE MUTANT BASED ON RAPD MARKERS Rustikawati, Rustikawati; Suprijono, Eko; Romeida, Atra; Herison, Catur; Sutjahjo, Surjono H.
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.102

Abstract

Gamma irradiation to induce mutation in plant has been used intensively since several decades ago.  On maize, 275 Gy gamma irradiation have been known to increase genetic variability indicated by their morphological variation.  Identification on genetic changes by molecular technique is important to answer whether there is mutation happening on DNA level of the plants.   The objective of this research was to identify RAPD marker polymorphism on gamma irradiation mutants compared to their parents.  The initial step of the research was to select random primers could positively amplify the maize DNA.  The result showed that selection on 60 random primers yielded 15 primers that positively amplified the maize DNA.  Amplification on both mutants and their parents by those 15 selected primers indicated that only 5 primers yielding polymorphism between mutants and their parents.  Polymorphisms on mutant G1, G3 and G6 were detected on one locus, meanwhile on mutant G7, G8 and G9 were on two loci.
Manually Feed NPK Fertigation Improves Growth and Yield of Chili Pepper Hybrids in Ultisol Herison, Catur; Rustikawati, Rustikawati; Hasanudin, Hasanudin; Suharjo, Usman K.J.; Handajaningsih, Merakati; Nursalim, Nursalim
Akta Agrosia Vol 23, No 1 (2020)
Publisher : Faculty of Agriculture, The University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/aa.23.1.19-26

Abstract

Extending the planting area to a less-fertile soil, such as Ultisols, become inevitable choice to increase the national chili pepper production in Indonesia as this types of soil is the largest part of dry land of Indonesia, about 29.7% or around 51 million ha.  However, efforts to increase its productivity by providing sufficient fertilizers application is most frequently inefficient because in the conventional fertilizer applications, broadcasted fertilizer on the soil bed, are not readily available for the plant as their solubility is limited under the plastic mulch and still in the form of granular until the end of planting season.  Fertigation, therefore, maybe the answer for this problem.  The objective of this study was to determine the best NPK fertigation method on four chili pepper hybrids.  The experiment was conducted in a randomized complete block design with three replication, and the treatments were arranged in a split plot design.  As the main plots were NPK fertigation intensity, i.e. 100%, 75%, 50%, 25% and 0% fertigation with the remnant fertilizers were circularly broadcasted surrounding the plant.  As the subplots were chili pepper hybrids, i.e. ‘Maxima’, H39, H14, and ‘UNIB CHR17’.  The results showed that there was no interaction effect of fertigation intensity indicated that all hybrid genotypes showed similar pattern of responses.  The H39 hybrid had better vegetative growth compared to other hybrids in term of plant height, dichotomous height, stem diameter, number of branches, plant fresh-weight.  Whereas the hybrid cultivar ‘Maxima’ showed the highest yield followed by H14 and cultivar ‘UNIB CHR17’.  The 100% fertigation was the best method of NPK fertilizer application for chili pepper in Ultisols.keywowrd: fertigation, chili pepper, fertilizer application, acidic soil
DNA MARKER-ASSISTED AND MORPHOLOGICAL SELECTION ON BC3 GENOTYPES SHORTCUT THE INTROGRESSION OF CMV TOLERANCE GENES ON CHILI PEPPER Catur Herison; Sri Winarsih; Merakati Handayaningsih; Rustikawati Rustikawati
AGRIVITA, Journal of Agricultural Science Vol 34, No 3 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i3.103

Abstract

Superior hybrid and CMV tolerant cultivar development requires a pair of high heterobeltiosis parents and both of which are CMV tolerant.  Gene introgression has to be accomplished if the tolerance does not exist in the parents. This research was objected to employ DNA markers and morphological traits on identifying the most resemble BC3 CMV tolerant individuals to their recurrent parents to shortcut the backcrossing cycle. This research used fifteen BC3 populations generated from crosses of hybrid parents PBC1354 and PBC378 with tolerant parents of C1024, C1042 and C1043.  The BC3 populations were previously selected for their CMV tolerance and were characterized by RAPD technique and morphological traits. Selection by means of both RAPD marker and morphological traits identified BC3 individuals 99.9% resemble to their recurrent parent. Genotypes B3A24-20, B3A29-13, B3A29-22, B3B12-13, B3B37-9, B3B12-25, B3C16-5, B3C16-16, B3C34-18 99.9% were similar to PBC378, and  B3D11-8, B3D11-17, B3D38-5, B3E12-17, B3E20-22, B3E31-19  were 99.9% similar to PBC1354.   Those genotypes were both similar to their recurrent parent and tolerance to CMV. The employed strategy shortcut CMV tolerance genes introgression through a backcross breeding.
KAJIAN GENETIK DAN SELEKSI GENOTIPE S5 KACANG HIJAU (Vigna radiata) MENUJU KULTIVAR BERDAYA HASIL TINGGI DAN SEREMPAK PANEN S. H. Sutjahjo; Rustikawati Rustikawati; A.W. Sandhi S.G.
Agrin Vol 11, No 1 (2007): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2007.11.1.59

Abstract

Tujuan penelitian ini adalah untuk melakukan studi genetik (pendugaan nilai heritabilitas, variabilitasgenetik, dan korelasi genetik), dan untuk melakukan seleksi indeks menuju pengembangan kultivar berdayahasil tinggi dan panen serempak. Tiga puluh empat genotipe kacang hijau ditanam dalam rancangan acakkelompok dengan tiga ulangan. Hasilnya menunjukkan bahwa buku total memiliki nilai duga heritabilitastinggi, dua belas karater lainnya memiliki nilai duga heritabilitas sedang hingga rendah. Total bukumenunjukkan variabilitas genetik luas. Tinggi tanaman, jumlah polong total, bobot polong, dan rasio bobotbiji terhadap polong menunjukkan korelasi genetik yang kuat terhadap hasil, dengan koefisien korelasigenetik berturut-tutur sebesar 0.672, 0.455, 0.912, dan 0.680. Genotipe 8, 14, betet, 1, dan 24 menunjukkannilai indeks seleksi yang lebih tinggi dibandingkan genotipe yang lain, dan karena itu genotipe tersebutdirekomendasikan untuk pengembangan selanjutnya.Kata kunci: kacang hijau, studi genetik, seleksi indeks ABSTRACTThe objectives of this research were to do genetic study (heritability estimation, genetic variability,and genetic correlation) and to do index selection for developing high yielding and concurrently harvestingcultivars. Thirty four mungbean genotypes were grown in a randomized complete block design with threereplications. The result showed that total node had high heritability values and 12 other characters hadmoderate to low heritability values. Total node showed broad genetic variability. Plant height, total pod, podweight, and ratio seed to pod weight showed strong genetic correlation with the yield; with geneticcoefficient correlation of 0.672, 0.455, 0.912, and 0.680 respectively. Genotype 8, 14, Betet, 1, and 24showed better selection index value than other genotypes, and, therefore, they were recommended for furtherdevelopment.Key words: mungbean, genetic study, index selection 
EVALUATION OF GROWTH AND YIELD PERFORMANCE ON INOCULATED CHILI PEPPER HYBRIDS BY CUCUMBER MOSAIC VIRUS Catur Herison; Merakati Handayaningsih; Fahrurrozi Fahrurrozi; Rustikawati Rustikawati
AGRIVITA Journal of Agricultural Science Vol 36, No 1 (2014)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v36i1.336

Abstract

Tolerance cultivar is a best control measure to reduce losses due to virus attack in the field.  Study on growth and yield of new cultivars compared to the commercial ones is an important step in cultivar development whether they will be accepted by farmers or not. The objective of this research was to study growth and yield of new chili pepper hybrids developed for CMV tolerance in comparison to a commercial and similar fruit size one. Twelve new hybrids and one commercial hybrid cultivar 'Prada', as a control, were evaluated in a completely randomized design with 3 replications. In the greenhouse experiment, H23 and H20 showed better vegetative growth compared to other hybrids and control. Hybrid H17 showed the highest yield followed by H5, H4 and H6.  Keywords.  growth, yield, chili pepper hybrids, CMV tolerance
PELATIHAN PENGOLAHAN KOPI HERBAL UPAYA OPTIMALISASI POTENSI DAN INOVASI USAHA KELOMPOK TANI DESA IV SUKU MENANTI KABUPATEN REJANG LEBONG Wulandari, Sri Wulandari; Alnopri, Alnopri; Rustikawati, Rustikawati; Ela Hasri Windari, Ela Hasri Windari; Fernandez, Regi
JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Vol 4, No 1 (2024): Sipissangngi Volume 4, Nomor 1, Maret 2024
Publisher : Lembaga Penelitan dan Pengabdian Masyarakat, Universitas Al Asyariah Mandar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35329/jurnal.v4i1.4942

Abstract

Pengabdian ini bertujuan memberikan pelatihan kepada kelompok tani Tunas Jaya dan Bima Saktal yang berada di Desa IV Suku Menanti berkaitan dengan pengolahan kopi herbal dalam upaya pengoptimalan potensi sumber daya lokal desa setempat, pengabdian juga memberikan dan menambah inovasi kelompok tani dari pengembangan produk guna meningatkan pendapatan usaha berbasis komoditas kopi. Metode pelaksanaan dengan penyuluhan, pelatihan, dan monitoring. Pengabdian masyarakat yang telah dilakukan di Desa IV Suku Menanti, dapat merefleksikan keingintahuan masyarakat mengenai pembuatan kopi herbal dengan varian rasa rempah. Terbukti dengan antusiasnya masyarakat dalam mempertanyakan hal-hal yang berkaitan dengan proses pengolahan  kopi herbal dan pemanfaatan tanaman herbal. Pelatihan meningkatkan pengetahuan manfaat dan cara pembuatan atau pengolahan kopi herbal yang semula tidak diketahui oleh anggota kelompok tani. Pelatihan memberikan manfaat melalui diversifikasi kopi bubuk herbal berhasil baik yang diindikasikan  oleh  tingkat  partisipasi  anggota  kelompok.  Program pemberdayaan masyarakat memberikan inovasi dari kopi biasa menjadi kopi herbal sehingga mendorong peningkatan perekonomian tanaman kopi yang merupakan unggulan lokal Desa IV Suku Menanti.
Pemanfaatan Limbah Kulit Kopi sebagai Cascara untuk Pemberdayaan Kelompok Tani di Desa IV Suku Menanti Wulandari, Sri; Fernandez, Regi; Windari, Ela Hasri; Alnopri, Alnopri; Rustikawati, Rustikawati
Jurnal Pengabdian Pada Masyarakat Vol 9 No 3 (2024): Jurnal Pengabdian Pada Masyarakat
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/jppm.v9i3.895

Abstract

Salah satu daerah penghasil kopi terbesar di Kabupaten Rejang Lebong adalah Desa Suku IV Menanti Kecamatan Sindang Dataran. Pengolahan biji kopi menghasilkan produk samping yaitu kulit kopi yang kemudian berpotensi menjadi limbah. Membantu petani kopi yang kekurangan pengetahuan dan keterampilan untuk mengolah limbah kulit kopi, telah diadakan pelatihan pembuatan cascara guna meningkatkan ekonomi dan kualitas lingkungan. Program ini bertujuan untuk memberdayakan anggota kelompok tani Bima saktal dan Tunas Jaya yang berada di Desa IV Suku Menanti, melalui penggunaan limbah kulit kopi untuk menghasilkan produk dengan nilai tambahan. Proses pelaksanaan program ini mencakup persiapan, pelaksanaan, pendampingan, pengawasan dan evaluasi, dan rencana keberlanjutan program. Hasil dari pelaksanaan kegiatan ini ialah menunjukkan hasil yang positif dengan motivasi peserta yang cukup antusias mengikuti kegiatan program ini. Kegiatan pengabdian masyarakat ini meningkatkan pengetahuan dan keterampilan masyarakat tentang pemanfaatan kulit kopi menjadi cascara, teknik-teknik pengolahan cascara, serta teknik menciptakan cascara yang berkualitas dan aman untuk dikonsumsi. Potensi keberlanjutan dari program ini didukung oleh adanya pendirian kedai kopi di desa setempat serta desa IV Suku Menanti sebagai desa wisata sehingga diharapkan membantu memasarkan produk cascara untuk mendukung keberlanjutan aspek sosial, ekonomi, dan lingkungan masyarakat setempat. One of the largest coffee-producing areas in Rejang Lebong Regency is Suku IV Menanti Village, Sindang Dataran District. Coffee bean processing produces a by-product, namely coffee skin, which then has the potential to become waste. To help coffee farmers who lack the knowledge and skills to process coffee skin waste, cascara-making training has been held to improve the economy and environmental quality. This program aimed to empower members of the Bima Saktal and Tunas Jaya farmer groups in Suku Menanti Village IV, through the used of coffee skin waste to produce products with added value. The implementation process of this program includes prepared, implemented, mentored, supervised, evaluated, and program sustainability planned. The results of the implementation of this activity are showing positive results with the motivation of participants who are quite enthusiastic about participating in this program. This community service activity increases community knowledge and skills about the use of coffee skins to become cascara, cascara processing techniques, and techniques for creating quality and safe cascara for consumption. The potential for sustainability of this program is supported by the establishment of coffee shops in the local village and Village IV Suku Menanti as a tourist village so that it is expected to help market cascara products to support the sustainability of the social, economic, and environmental aspects of the local community.
Assessment on Genetic Diversity and Relationship of 19 Bird Pepper Genotypes Based on Morphological and SSR Markers Rustikawati, Rustikawati; Herison, Catur; Sutrawati, Mimi; Prameswari, Wuri
AGRIVITA Journal of Agricultural Science Vol 47, No 1 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i1.4447

Abstract

A comprehensive assessment of genetic diversity offers invaluable insights to breeders. This study aims to analyze the genetic diversity of 19 cayenne pepper genotypes using morphological and SSR markers.  Morphological markers are essential for evaluating visible traits while SSR markers provide deeper insights into genetic variation. Using both morphological and SSR markers provides a more robust, multidimensional approach to understanding genetic diversity. Morphological characterization was conducted on 19 bird pepper genotypes using 30 distinct traits, further divided into 95 sub-traits, to evaluate vegetative and generative growths. Molecular profiling was carried out using 10 SSR primers. The SSR analysis yielded 80 scorable bands, of which 72 were polymorphic. The high polymorphic information content suggests that these markers were effective in differentiating the genotypes.   The genetic diversity within the population was substantially high, providing valuable genetic material for breeding programs. Genetic similarity analysis divided the population into six distinct groups. Among them, Group I (genotype A05 Taiwan) and Group VI (genotype A02, Bengkulu Indonesia) formed unique, solitary groups, distinguishing them from other clusters. This information is highly beneficial for bird pepper cultivar development, emphasizing the importance of incorporating additional traits to align with the cultivar development of high yielding and saline-stress tolerance.
Identifikasi Genetik Kultivar Padi Gogo dengan Menggunakan Marka RAPD Simarmata, Marulak; Rustikawati, Rustikawati
Akta Agrosia Vol 18 No 2 (2015)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4329.729 KB)

Abstract

A research was conducted to identify the genetics of upland rice cultivars using random amplified polimorphic DNA (RAPD) markers. Five out of 60 random primers that capable to amplify the most number of markers were OPE-07 (AGATGCAGCC), OPE-15 (ACGCACAACG), OPH-13 (GACGCCACAC), OPH19 (CTGACCAGCC), and OPM-02 (ACAACGCCTC). The number of markers amplified were 5, 6, 8, 8, and 7, respectively. RAPD-PCR using these 5 primers arranged to DNA templates of 41 rice cultivars amplified 1127 RAPD markers. Polymorphic information content (PIC) of each primer were 0.79, 0.80, 0.87, 0.87, and 0.72, respectively. Cluster analysis using a UPGMA dendogram showed that the 41 cultivars were grouped into 9 clusters with genetic similarity index more than 90 percent.
Co-Authors A.A. Ketut Agung Cahyawan W A.W. Sandhi S.G. Adeputri, Eriana Alnopri Alnopri, Alnopri Atra Romeida Bertham, Yudhy H Catur Herison Dewi Liesnoor Setyowati Dwi Wahyuni Ganefianti, Dwi Wahyuni Dwitama, Niko EDHI TURMUDI Eko Suprijono Ela Hasri Windari, Ela Hasri Windari Fahrurrozi Fahrurrozi Fernandez, Regi Gustian, Meko Hasanudin Hasanudin Herison, Catur HESTI PUJIwATI Hindarto, Kanang Setyo Indres, Indres Inoriyah, Entang Luwih, Bagus Edi Marulak Simarmata Merakati Handajaningsih Merakati Handayaningsih Mimi Sutrawati Muhammad Chozin, Muhammad Mukhtasar, Mukhtasar Nurjanah, Uswatun Nursalim Nursalim Reny Herawati S. H. Sutjahjo Saputra, Pringky Okta Sianipar, Mangara W Sri Wulandari Sri Wulandari Wulandari, Sri Wulandari Suharjo, Usman K.J. Sukisno Sukisno, Sukisno Surjono H. Sutjahjo Surmaini, Ervi Suryati, Dotti Windari, Ela Hasri Wuri Prameswari Yulian, Yulian