Indonesian Journal of Biotechnology
Vol 24, No 2 (2019)

Analysis of ethylene biosynthesis gene expression profile during titanium dioxide (TiO2) treatment to develop a new banana postharvest technology

Fenny M Dwivany (School of Life Science and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung 40132, Indonesia
ForMIND Institute, Bandung, Indonesia
Bali International Research Center for Banana, Universitas Udayana, Bukit Jimbaran, Kuta Selatan, )

Rizkita R Esyanti (School of Life Science and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung 40132, Indonesia
Bali International Research Center for Banana, Universitas Udayana, Bukit Jimbaran, Kuta Selatan, Badung, Bali 80361, Indonesia)

Veinardi Suendo (Chemistry Department, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung 40132, Indonesia
Research Center for Nanoscience and Nanotechnology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung 40132, Indonesia)

Aksarani ‘Sa Pratiwi (School of Life Science and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung 40132, Indonesia)
Annisa A Putri (School of Life Science and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung 40132, Indonesia
Bali International Research Center for Banana, Universitas Udayana, Bukit Jimbaran, Kuta Selatan, Badung, Bali 80361, Indonesia
Bioscien)



Article Info

Publish Date
09 Dec 2019

Abstract

Banana is an important crop that demands proper methods in postharvest handling. As a climacteric fruit, thebanana fruit ripening process is affected by ethylene. Several methods have been developed to extend the shelf life of a banana, such as using ethylene scrubbers. In this study, ttanium dioxide (TiO2), a photocatalyst, was used as an alternatve method to delay the fruit ripening process. The effect of TiO2 on the ripening‐related gene MaACS1 was investgated. Banana fruits were placed in a TiO2‐coated glass chamber and observed for ten days. Fruit ripening in the treated chamber was delayed for eight days compared to the control. Total RNA was extracted from control and TiO2‐treated fruit pulp and synthesized into cDNA. Reverse transcripton PCR was performed to investgate the gene expression, which showed that MaACS1 expression was relatvely lower than treated control. The fnding of these studies suggested that the TiO2 chamber has the potental to extend the shelf life of banana by delaying its ripening process and decreasing the expression of MaACS1. To the best of our knowledge, no previous study has investgated the effect of TiO2 on the expression of genes related to banana fruit ripening.

Copyrights © 2019






Journal Info

Abbrev

ijbiotech

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...