An experiment on isolate Symbiodinium sp. from Hippopus hippopus was carried out to determine growth medium and optimum salinity for in vitro culture of the symbiont. The split-plot factorial design with two factors was applied with 3 replications. The first factor was medium with 2 levels, namely f/2 and modified GPM. The second factor was salinity with five levels i.e. 20, 25, 30, 35 and 40 PSU. All treatments were incubated under continuous light (2600 lux) and 25°C. The maximum growth was reached at day 16 and there was significant interaction (p<0.05) between medium and salinity. The maximum density (64.8 x 104cell/ml) was found in media f/2 and salinity 35 PSU.
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