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All Journal Marine Research in Indonesia
Hutagalung, Rory A.
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Earth & Planetary Sciences

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EFFECT OF SALINITY AND GROWTH MEDIUM ON Symbiodinium sp. ISOLATED FROM GIANT CLAM Panggabean, Maria Goretti Lily; Hutagalung, Rory A.; Ayu, Eveline
Marine Research in Indonesia Vol 34 No 2 (2009)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4527.442 KB) | DOI: 10.14203/mri.v34i2.513

Abstract

An experiment on isolate Symbiodinium sp. from Hippopus hippopus was carried out to determine growth medium and optimum salinity for in vitro culture of the symbiont. The split-plot factorial design with two factors was applied with 3 replications. The first factor was medium with 2 levels, namely f/2 and modified GPM. The second factor was salinity with five levels i.e. 20, 25, 30, 35 and 40 PSU. All treatments were incubated under continuous light (2600 lux) and 25°C. The maximum growth was reached at day 16 and there was significant interaction (p<0.05) between medium and salinity. The maximum density (64.8 x 104cell/ml) was found in media f/2 and salinity 35 PSU.
Co-Authors EVELINE AYU Panggabean, Maria Goretti Lily