HAYATI Journal of Biosciences
Vol. 17 No. 2 (2010): June 2010

Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia

DEWI FITRIANI (Research Center for Biotechnology, Indonesia Institute of Science)
BUDI SAKSONO (Research Center for Biotechnology, Indonesia Institute of Science)

Article Info

Publish Date
17 Jun 2010

Abstract

L-arabinose isomerase is an enzyme converting D-galactose to D-tagatose. D-tagatose is a potential sweetener-sucrose substitute which has low calorie. This research was to clone and sequence araA gene from marine bacterial strain Geobacillus stearothermophilus isolated from Tanjung Api Poso Indonesia. The amplified araA gene consisted of 1494 bp nucleotides encoding 497 amino acids. DNA alignment analysis showed that the gene had high homology with that of G. stearothermophilus T6. The enzyme had optimum activity at high temperature and alkalin condition.

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Journal Info
HAYATI Journal of Biosciences
Website

Abbrev

hayati

Publisher

Institut Pertanian Bogor

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...