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DEWI FITRIANI
Research Center for Biotechnology, Indonesia Institute of Science
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia DEWI FITRIANI; BUDI SAKSONO
HAYATI Journal of Biosciences Vol. 17 No. 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.138 KB) | DOI: 10.4308/hjb.17.2.58

Abstract

L-arabinose isomerase is an enzyme converting D-galactose to D-tagatose. D-tagatose is a potential sweetener-sucrose substitute which has low calorie. This research was to clone and sequence araA gene from marine bacterial strain Geobacillus stearothermophilus isolated from Tanjung Api Poso Indonesia. The amplified araA gene consisted of 1494 bp nucleotides encoding 497 amino acids. DNA alignment analysis showed that the gene had high homology with that of G. stearothermophilus T6. The enzyme had optimum activity at high temperature and alkalin condition.
Co-Authors BUDI SAKSONO