Pachybasin, a Major Metabolite from Culture Broth of Endophytic Coelomyceteous AFKR-18 Fungus isolated from a Yellow Moonsheed Plant, Arcangelisia flava (L.) Merr.

DEWI WULANSARI (Phytochemistry Laboratory, Research Center for Biology, Indonesian Institute of Sciences Jalan Raya Bogor Km. 46, Cibinong 16911, Indonesia)
YULIASRI JAMAL (Phytochemistry Laboratory, Research Center for Biology, Indonesian Institute of Sciences Jalan Raya Bogor Km. 46, Cibinong 16911, Indonesia)
. PRAPTIWI (Phytochemistry Laboratory, Research Center for Biology, Indonesian Institute of Sciences Jalan Raya Bogor Km. 46, Cibinong 16911, Indonesia)
ANDRIA AGUSTA (Phytochemistry Laboratory, Research Center for Biology, Indonesian Institute of Sciences Jalan Raya Bogor Km. 46, Cibinong 16911, Indonesia)

HAYATI Journal of Biosciences
Vol. 21 No. 2 (2014): June 2014

Endophytic fungi have been known to produce a broad range of biologically active secondary metabolites. One endophytic filamentous fungus, Coelomycetes AFKR-18, isolated from the young stems of a yellow moonsheed plant, Arcangelisia flava, has been found to produce pachybasin when placed in a liquid medium. The chemical structure of pachybasin was deduced from MS, 1D-, 2D-NMR spectrum analysis, and from reference data. On a micro-dilution test, pachybasin showed antimicrobial activities against E. coli, B. subtilis, M. luteus, S. cerevisiae, C. albicans, A. niger, and A. flavus, with MIC values of 64.0 µg/mL, and against S. aureus and F. oxysporum with MIC values of 32.0 and 16.0 µg/mL respectively.