Analisis Keragaman Genetik Isolat Bakteri Xanthomonas oryzae pv. oryzae dari Jawa Barat dan Jawa Tengah Berdasarkan Analisis ARDRA Gen 16SrRNA

Yadi Suryadi (Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor)
Dwi N. Susilowati (Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor)
Puji Lestari (Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor)
Sutoro Sutoro (Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor)
Ifa Manzila (Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor)
Trini Suryani Kadir (Balai Besar Penelitian Tanaman Padi, Subang)
Sheryn Sunni Albani (Institut Pertanian Bogor)
I Made Artika (Institut Pertanian Bogor)

Jurnal Fitopatologi Indonesia
Vol 10 No 2 (2014)

Fifteen Xanthomonas oryzae pv. oryzae isolates from several regions in West Java and Central Java have been studied for their genetic diversity using amplified ribosomal DNA restriction analysis (ARDRA) 16SrRNA gene.Total of 13 ARDRA patterns from digestion of DNA with RsaI restriction enzyme was obtained, indicated higher genetic diversity of X. oryzae pv. oryzae. Phylogenetic analysis revealed a close genetic relationships among isolates. Isolates X. oryzae pv. oryzae 1/96pml and 61pml are closely related with isolates 29d, 59pml, and 60pml although they are isolated from different locations. The other closely related isolates were between X. oryzae pv. oryzae 5mgl with 23d, 38d, 10sbg, 8myd; while 6klt was close to 3ind and 2kr. The result of X. oryzae pv. oryzae genetic diversity can be used as a reference for strain or/isolates selection for development ofbacterial leaf blight resistance in Indonesia.