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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Gizi dan Pangan Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Jurnal Farmasi Udayana CHEMISTRY PROGRESS Jurnal Pendidikan Bahasa dan Sastra Indonesia Undiksha Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Current Biochemistry The Journal of Pure and Applied Chemistry Research Indonesian Journal of Biotechnology BERITA BIOLOGI ANNALES BOGORIENSES FITOFARMAKA: Jurnal Ilmiah Farmasi Menara Perkebunan Jurnal Jamu Indonesia Sainstek : Jurnal Sains dan Teknologi Jurnal Pusat Inovasi Masyarakat Jurnal Ilmu Kehutanan Molekul: Jurnal Ilmiah Kimia Journal of The Indonesian Society of Integrated Chemistry Indonesian Journal of Jamu

I MADE ARTIKA

Department Of Biochemistry, Faculty Of Mathematics And Natural Sciences, Darmaga Campus, IPB University, Bogor, 16680, Indonesia

Author-ID : 234078

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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AKTIVITAS AMILASE BAKTERI YANG DIISOLASI DARI SUMBER AIR PANAS CISEENG BOGOR Amri, Erismar; Widhyastuti, Nunuk; Artika, I Made
Sainstek : Jurnal Sains dan Teknologi Vol 2, No 1 (2010)
Publisher : IAIN Batusangkar

This research was aimed at studying the activities of amylase produced by isolate bacteria which were isolated from hot water resource, Ciseeng Bogor. Four among the nine isolate bacteria grown on NA media showed the activities of amylase. The character of Amylase was analyzed in 2 isolates, A1 and A3. Isolate A1 was optimally active at pH 3, 55 C, meanwhile isolate A3 was optimally active at pH 4, 50 C. The amylase resulted from either isolate A1 or isolate A3 was acid amylase. Both of the isolates were relatively stable at pH acid, whereas the amylase resulted from A3 was not stable on pemanasan in 30 minutes, and the amylase from A1 increased after being incubated at more than 60 C in 30 minutes. In conclusion, isolate A1 identified as Bacillus Coagulans indicated the optimal activities when it was grown at pH 6 and at the 14th hour of incubation.Key words: amylase, isolation, enzyme

IDENTIFIKASI GEN TERMOASIDOFILIK ALKOHOL 1 DEHIDROGENASE PADA BACILLUS SP-PJV MS, Yulia Atika; Artika, I Made; Nurhidayat, Novik
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1872

One of the constrains in the conversion process of biomass to bioethanol is the unoptimum technology for the conversion process.One of the causes is the difference between optimum temperature for enzymatic hydrolytic saccharification and for fermentation.Enzymatic hydrolysis requires high temperature and acidic pH, while fermentation operates at mild condition.Hence, thermophilic fermentative microbes are needed so that simultaneous saccharification and fermentation processes can be carried out.Alcoholdehydrogenase(ADH), an enzyme functions in the final step of fermentation, catalyzes reduction of acetaldehydeto ethanol so that it being one of indicator fermentative microbial.Protium javanicum, a typical fruit from Lombok grow at high temperature and has sour taste so that it predicted has a thermo acidophilic ADH.This study was aimed toidentify gene encoding ADHin Protiumjavanicum(Pjv) microbial isolates.ADH gene identification was carried out by DNA amplification using spesific adh primers inqPCR machine. Results showed that all isolates tested had adh gene and that of Bacillus sp-Pjv was the most efficiently amplified. Results of fermentationtest also showed that the Bacillus sp-Pjv isolate was a better ethanol-producer than the others.

PROPOLIS SEBAGAI ALTERNATIF BAHAN ANTIKARIES GIGI Hasan, A. E. Zainal; Artika, I Made; K, Popi A; Lasmiyanti, Metty
CHEMISTRY PROGRESS Vol 4, No 1 (2011)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.4.1.2011.26504

ABSTRACTZainal Hasan A. E. et al., 2011. Propolis as alternative additive anti-cariesPeriodontal disease included dental caries still the major problem in the world. Usually commercial tooth pastecontains fluoride which has an important role to prevent tooth from damage. Contain fluoride overdose causedfluorosis, bone damage and anemia. Therefore, the propolis was being tested as alternative additive anti cariesto prevent the growth of cariogenics bacteria (Streptococcus mutans). This research studied propolis activityantibacterial and the minimum inhibitory concentration (MIC) of propolis against S. mutans.Ethanolic extract of propolis (EEP) contained various bioactive compound such as flavonoid, tannine, alkaloid,triterpenoid and saponin based on phytochemistry analyses. The extract effectivities are 96.5% relative to thepropolis X, 41.04% relative to amphicillin 10 mg/ml and 240.57% relative to NaF 3000 ppm. The MIC of theextract is 3.13%. Therefore, propolis extract can be used asa additive anti caries element in tooth paste.Statistical analyses showed that 100% EEP, X propolis and amphicillin 10 mg/ml had the ability to decrease S.mutans colonyKeywords : propolis, anti-caries, ethanolic extract

PENERAPAN TEKNOLOGI NANOPARTIKEL UNTUK SEDIAAN OBAT (ANTIBIOTIK BERBASIS BAHAN ALAM, PROPOLIS TRIGONA SPP) Hasan, H. A. E. Zainal; Artika, I Made; Fahri, Vita Rosaline; Sari, Nurmala
CHEMISTRY PROGRESS Vol 5, No 1 (2012)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.5.1.2012.655

Zainal Hasan dkk., 2012. Penerapan teknologi nanopartikel untuk sediaan obat (antibiotik berbasis bahanalam, Propolis Trigona spp.). Propolis Trigona spp telah diteliti dapat menghambat pertumbuhan bakteri patogen seperti Escherichia coli.Senyawa Flavonoid yang terkandung dalam propolis diduga berperan sebagai antibakteri. Jika ukuran partikelmakin kecil maka luas permukaan partikel makin besar sehingga laju dari larutan semakin meningkat danmempercepat penyerapan obat melalui peredaran darah sehingga efek terapeutiknya lebih cepat tercapai.Salah satu cara yang dapat dilakukan untuk memperkecil ukuran partikel guna meningkatkan kelarutan danpenyerapan suatu sediaan farmasi adalah dengan menggunakan nanoteknologi.Pada penelitian ini telah dirancang sediaan propolis dalam bentuk nanopartikel. Tujuan penelitian ini adalahmembuat nanopartikel dari propolis Trigona spp. Proses pembuatan nanopropolis yaitu dengan cara penyalutandan homogenizer pada kecepatan tinggi. Telah dilakukan uji aktivitas dari nanopartikel propolis sebagaiantibakteri dan menentukan konsentrasi hambat tumbuh minimum propolis nanopartikel terhadap bakteriEscherichia coli secara in vitro. Dari hasil penelitian ini menunjukkan bahwa propolis Trigona spp dapat dibuatdalam bentuk nanopartikel. Hasil Scanning Electron Microscopy menunjukkan adanya ukuran diameternanopropolis sebesar 100 ? 322 nm. Hasil uji aktivitas antibakteri menunjukkan pada konsentrasi 10% - 0,02%nanopropolis aktif terhadap E. coli sedangkan propolis bukan nanopartikel aktif sampai konsentrasi 0,15%.Dengan demikian KHTM ?propolis bukan nanopartikel? dicapai pada konsentrasi 0,15%, sedangkannanopropolis KHTM nya lebih rendah dari 0,02%. Propolis nanopartikel lebih efektif dibandingkan ?propolisbukan nanopartikel? dalam menghambat pertumbuhan E. coli.Kata kunci : propolis, nanopartikel, E.coli, KHTMZainal Hasan et al., 2011. Application of nanoparticle technology for medicine preparate (Antibiotic base onnatural product, Propolis Trigona spp.). Trigona spp propolis has been evaluated can inhibit Escherichia coli. Flavonoid in propolis suggested act asantibacterial. If particle size smaller, then its surface area is bigger, so the rate of drugs absorption wereincreased trough blood circulation and its therapy effect can be achieved faster. One of resize way to reduceparticle size to increase its solubility dan absorption pharmaceutical is using nanotechnology.This research has been designed propolis in nanoparticle size. The aims of this research is to makenanoparticle propolis from Trigona spp. The process to make nanopropolis was covered it and homogenize inhigh speed. Nanopropolis activity evaluation has been done as antibacterial and determined its minimuminhibition concentration on Escherichia coli (in vitro). Trigona spp propolis can be made to nanoparticle. ElectronMicroscopy scanning shows nanopropolis diameter was 100 ? 322 nm. Antibacterial activity evaluation showson nanoproposis concentration 10% - 0,02% active on E. Coli, although nonnanopropolis active onconcentration 0,15%. Nanopropolis is more effective than non nanopropolis to inhibit E. coli.Keywords : propolis, nanopartikel, E.coli, KHTM

PENERAPAN PENDEKATAN READER RESPONS DALAM MENINGKATKAN KEMAMPUAN MEMBACA NOVEL SISWA SMP MUTIARA SINGARAJA Harsana, Ngurah; Artika, I Made; Sudiana, I Nyoman
Jurnal Pendidikan Bahasa dan Sastra Indonesia Undiksha Vol 8, No 1 (2018)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpbs.v8i1.20590

Penelitian tindakan kelas yang dilakukan di SMP Mutiara Singaraja ini bertujuan (1) mengidentifikasi langkah-langkah penerapan pendekatan reader respons dalam meningkatkan kemampuan membaca novel siswa SMP Mutiara Singaraja, (2) mengetahui peningkatan kemampuan membaca novel siswa dengan menggunakan pendekatan reader respons di SMP Mutiara Singaraja, dan (3) mengetahui respons siswa terhadap penerapan pendekatan reader respons dalam pembelajaran memahami buku novel. Penelitian ini dilaksanakan dengan dua siklus dan menggunakan lima tahap penelitian tindakan kelas, yakni (1) refleksi awal, (2) perencanaan tindakan, (3) pelaksanaan, (4) observasi dan evaluasi, dan (5) refleksi. Data dikumpulkan dengan metode observasi, tes, angket/kuisioner, serta wawancara dan dianalisis secara deskriptif kuantitatif dan kualitatif. Hasil penelitian menunjukkan nilai rata-rata siklus I adalah 69,05 atau mengalami peningkatan 8,35% dari nilai sebelumnya 60,70. Nilai rata-rata siklus II adalah 79,85 atau meningkat sebesar 10,8% dari siklus I dan meningkat 19,15% dari nilai rata-rata prasiklus. Perolehan rata-rata respons siswa pada siklus I sebesar 43,05 dalam kategori positif dan pada siklus II mengalami peningkatan sebesar 1,6%, sehingga menjadi 44,65 dalam kategori positif.

Antibacterial Activity of Propolis Produced by Trigona spp. Against Campylobacter spp. AMIN FATONI; I MADE ARTIKA; AHMAD ENDANG ZAINAL HASAN; KUSWANDI KUSWANDI
HAYATI Journal of Biosciences Vol. 15 No. 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

Propolis is believed to have antimicrobial, anti-inflammatory and immunostimulating activities. The objective of this study was to investigate the antibacterial activity of ethanol extract propolis (EEP) of Trigona spp. from Bukittinggi West Sumatera against Campylobacter spp. Antibacterial activity of the EEP was measured by disc diffusion method. The compound groups of the propolis were also analyzed on the existence of alkaloids, flavonoids, saphonins, tannins, steroids, and terpenoids. This study revealed that the EEP of Trigona spp. shows an antibacterial activity on Campylobacter spp. The compound groups detected in the EEP were flavonoids and tannins, suggesting that the antibacterial activity of propolis of Trigona spp. may be due to these compounds. Key words: Trigona spp., antibacterial activity, Campylobacter spp

The Use of HIS6 Gene as a Selectable Marker for Yeast Vector I MADE ARTIKA
HAYATI Journal of Biosciences Vol. 16 No. 1 (2009): March 2009
Publisher : Bogor Agricultural University, Indonesia

The yeast Saccharomyces cerevisiae HIS6 gene has been shown to be functional as a selectable marker for selecting and maintaining a yeast vector in yeast S. cerevisiae host cells. The yeast HIS6 gene encodes an enzyme involved in the yeast histidine biosynthesis. The yeast HIS6 gene was cloned into a yeast expression vector. The resultant recombinant plasmid was introduced into yeast host cells defective in endogenous HIS6 gene. The functionality of the HIS6 gene as a selectable marker was tested by growing transformed cells on selective minimum medium lacking histidine supplementation. Key words: HIS6 gene, selectable marker, vector, transformation, yeast

Allotopic Expression of a Gene Encoding FLAG Tagged-subunit 8 of Yeast Mitochondrial ATP Synthase I MADE ARTIKA
HAYATI Journal of Biosciences Vol. 13 No. 1 (2006): March 2006
Publisher : Bogor Agricultural University, Indonesia

Subunit 8 of yeast mitochondrial ATP synthase is a polypeptide of 48 amino acids encoded by the mitochondrial ATP8 gene. A nuclear version of subunit 8 gene has been designed to encode FLAG tagged-subunit 8 fused with a mitochondrial signal peptide. The gene has been cloned into a yeast expression vector and then expressed in a yeast strain lacking endogenous subunit 8. Results showed that the gene was successfully expressed and the synthesized FLAG tagged-subunit 8 protein was imported into mitochondria. Following import, the FLAG tagged-subunit 8 protein assembled into functional mitochondrial ATP synthase complex. Furthermore, the subunit 8 protein could be detected using anti-FLAG tag monoclonal antibody. Key words: allotropic expression, ATP synthase, mitochondria, yeast

Fractionation and Characterization of Tannin Acyl Hydrolase from Aspergillus niger YUNITA ARIAN SANI ANWAR; I MADE ARTIKA; HASIM DANURI
HAYATI Journal of Biosciences Vol. 16 No. 3 (2009): September 2009
Publisher : Bogor Agricultural University, Indonesia

We previously produced tannin acyl hydrolase (tannase) from Aspergillus niger isolated from cacao pod. In the present study the enzyme was subjected to fractionation by ammonium sulphate followed by dialysis process. The saturation level of ammonium sulphate used was 30-80% where the best enzyme activity was obtained at the saturation level of 60%. Compared to that of crude enzyme, specific activity of tannase after dialysis was four folds. Characterization results showed that optimum activity was at 35-50 oC and pH 6. Tannase was activated by K+ and Na+ at concentration of 0.01 and 0.05 M respectively. Mg2+ was found activate tannase only at 0.01 M. Addition of metal ions like Zn2+, Cu2+, Ca2+, Mn2+ and Fe2+ inhibited the enzyme activity. Kinetics analysis of various substrates tested showed that the Km value of tannic acid and gallotannin was 0.401 and 6.611 mM respectively. Vmax value of tannic acid was 10.804 U/ml and of gallotannin was 12.406 U/ml. Based on Michaelis-Menten constant (Km), the tannase obtained in the present study was more active in hydrolysing depside bonds rather than ester bonds. Key words: tannase, fermentation, fractionation, characterization, Aspergillus niger

Bioenergetic Consequences of FLAG Tag Addition to the C-Terminus of Subunit 8 of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase I MADE ARTIKA
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

The yeast mitochondrial F1F0-ATP synthase is a multisubunit complex that contains at least 17 different subunits. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. FLAG tag addition to subunit 8 protein potentially facilitate elucidation of its topology, structure, and function. It has been shown that following incorporation of FLAG tag to its C-terminus, subunit 8 still assemble into functional ATP synthase complex. In order to analyze bioenergetic consequences of the FLAG tag addition, a yeast strain expressing FLAG tagged-subunit 8 was subjected to cellular respiration assays. Results obtained showed that addition of FLAG tag to the C-terminus of subunit 8 does not impair its proper functioning. The FLAG tag system, therefore, can be employed to study subunit 8’s detailed structure, topology, and function.

Co-Authors . SURYANI A. E. Zainal Hasan AA Sudharmawan, AA Abdul Choliq ADINDA VIRGINIA DWI SETYO Agung Eru Wibowo AHMAD ENDANG ZAINAL HASAN AHMAD ENDANG ZAINAL HASAN Ahmad Sulaeman Akhmad Endang Zainal Hasan Amanda, Nisa Widya AMIN FATONI Andani, Gita Putri Andita Fitri Mutiara Rizki, Andita Fitri Mutiara Antonius Padua Ratu Apipah Aprianti APON ZAENAL MUSTOPA Apon Zaenal Mustopa Arya Arendra Asri Sulfianti Azmi Azhari Azmi Azhari, Azmi Azmi, Wihda Aisarul BUGI RATNO BUDIARTO DEDI JUSADI Denny Irawati Desi Purwaningsih Dewi Sukma Dhani Luthfi Ramadhani DIMAS ANDRIANTO Djarot Sasongko Hami Seno Dodi Safari Dodi Safari Dodi Safari Dodi Safari Dwi N. Susilowati Dzihan Dinar Rabani Eliza Halim Erismar Amri Erlank Bagjavicenna Erna Puspasari Evi Nur Qolbaini Fatriani, Rizka Fina Febrianti Firda DIMAWARNITA Fri Rahmawati Gusnia Meilin Gholam Gusnia Meilin Gholam Gusnia Meilin Gholam H. A. E. Zainal Hasan Hafizh Zahra Hani 'Athiyya Rafi Hardinsyah Harsana, Ngurah HARTUTIK EKA SUSANTI HARYANTO SUSILO Hasim - HASIM DANURI Hayatul Rahmi Herti Sugiarti Herti Sugiarti, Herti Hyakansa HANIF Ifa Manzila Iman Akhyar Firdausy Iman Rusmana Inawati Inawati Jajang Suhyana K, Popi A Kurnia Agustini Laita Nurjanah Laita Nurjanah, Laita LAKSMI AMBARSARI Lasmiyanti, Metty Lusiana Kresnawati Hartono Luzicoiij, Michael Edison M. Zairin Junior M.Pd Prof. Dr. I Nyoman Sudiana . Mala Nurilmala MARIA BINTANG Meilisza, Nina Melva Louisa Mirza Dikari Kusrini MS, Yulia Atika Muhaimin Muhaimin MUHAMMAD AGUS SUPRAYUDI Muhammad Dailami, Muhammad Muhammad Nafiz Nisa Widya Amanda Noorwati Sutandyo Norman Razief Azwar Norman Razief Azwar Novik Nurhidayat Novik Nurhidayat Nuke Annisa Nasution NUNUK WIDHYASTUTI Nur Bambang Priyo Utomo Nur Hasanah Nurmala Sari Nurul Khumaida Perkasa Arian Puji Lestari Rahadian Pratama Rahmawati, Fri Ramadhani Malik Abdillah Rava Raisha Putra Resti Rahmawati Putri Ridwan Putra Firmansyah Rini Kurniasih, Rini Riyan Alifbi Putera Irsal Rizka Fatriani Roedhy Poerwanto Septiany C. Palilingan Sheryn Sunni Albani Siagian, Putri Junita Siregar, Josephine Elizabeth Siti Nurjanah Siti Nurjanah Soekarno Mismana Putra Soekarno Mismana Putra, Soekarno Mismana Sogandi Sogandi Sogandi Sogandi Sudarsono Suharyanto Suharyanto Sulistiani sulistiani Suryani Suryani Suryani Suryani Suryani Suryo Wiyono Sutoro Sutoro Syaeful Abidin Syamsul Falah Tatik Khusniati Tetty Chaidamsari Tetty Chaidamsari, Tetty Tri Panji Trini Suryani Kadir Vita Rosaline Fahri Waras Nurcholis Wasrin Syafii Wijiastuti Wijiastuti Yadi Suryadi Yahdiana Harahap Yulianto YUNITA ARIAN SANI ANWAR

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