Natural Science: Journal of Science and Technology
Vol 8, No 2 (2019): Volume 8 Number 2 (August 2019)

INDUKSI KALUS TANAMAN KENTANG DOMBU (SOLANUM TUBEROSUM L.) SECARA IN VITRO DENGAN PEMBERIAN ZPT 2,4-D (DICHLOROPHENOXY ACETID ACID)

Idris, Siti RLR (Unknown)
Paserang, Asri Pirade (Unknown)



Article Info

Publish Date
29 Aug 2019

Abstract

This research was aimed to determine the effect of the PGR 2.4-D (Dichlorophenoxy Acetid Acid) in various concentrations on induceing callus of Dombu potato (Solanum tuberosum L.). This research was performed based on Completely Randomized Design (RAL) with 6 treatments and 3 times repetation, so there were 18 experiment units. Each experiment used 3 explants so as there were 54 explants. The combination of concentration of the tested PGR in the culture media were T1 = MS0+ 2.4-D 0 ppm (control), T2 = MS0+ 2.4-D 0.5 ppm, T3 = MS0+ 2.4-D 1 ppm, T4= MS0+ 2.4-D 1.5 ppm, T5 = MS0+ 2.4-D 2 ppm and T6 = MS0+ 2.4-D 2.5 ppm. The results showed that callus induction was appeared in the concentration of 1.0, 1.5, 2.0, and 2.5 ppm. Callus was generally formed on the Day-8 after plantation. Callus color was mostly greenish transparent, callus texture was mostly crumb type, and callus formation percentage was almost 100%. The best media for inducing the callus was in treatment T4 (1.5 ppm 2.4-D), it referred to the formed callus biomass

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Journal Info

Abbrev

ejurnalfmipa

Publisher

Subject

Computer Science & IT

Description

Jurnal Natural Science online diterbitkan oleh Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) Universtas Tadulako. Jurnal ini menerbitkan artikel-artikel ilmiah hasil penelitian dalam bidang-bidang ilmu pengetahuan dan ...