ISOLATION, IDENTIFICATION AND ANALYSIS OF SHORT INTERSPERSED NUCLEAR ELEMENT (SINE) FROM VARANUS KOMODOENSIS

Widya Asmara (Bagian Mikrobiologi Fakultas Kedokteran Hewan UGM Yogyakarta)

Jurnal Sain Veteriner
Vol 17, No 1 (1999)

All vertebrata genomes studied so far contain SINEs, that consti tute up to 5% of the total genome. Most of the SINE sequences studied so far are mammalian genomes. There Is very limited information about SINE sequences from rep­tiles, especially of Varanus family.Isolation of SINE sequences were carried out by con­structing the komodo genomic library in E. coli. Suspected positive clones were detected by genome to genome hybridiza­tion. The SINE sequences identification were carried out by enzymatic method, and the DNA sequences were analysed with the GENETYX-SV program-Up to now there are 2 SINE sequences from komodo genome have been isolated and identified. Their sequences homo logy is 85-2% From Database search analysis with the GenBank indicated that those SlNEs were not evolved from any spe­cific tRNA gene.