RAPID CONFIRMATION OF RABIES ANTIGEN IN FORMALIN-FIXED PARAFIN-EMBEDDED TISSUE BY IMMUNOPATHOLOGIC DIAGNOSIS

R. Wasito (Department of Pathology Faculty of Veterinary Medicine, Gadjah Mada University Yogyakarta)
Hastari Wuryastuti (Veterinary Internal Medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta)

Jurnal Sain Veteriner
Vol 15, No 1&2 (1996)

Skunks experimentally infected with street virus rabies intramuscularly were used to demonstrate rabies viral antigen in paraffin-embedded tissue by immunopathology diagnosis approach, the avidin biotin complex (ABC) method. The use of ABC method provides a simple and sensitive method to localize rabies virus antigen in formalin- fixed tissues. The results of the present study also demonstrated that a procedures using formalin-fixed parafin-embeded tissue and stained immunopathologicalH by the ABC method was an excellent method for both preservation of morfological details of ceils/tissues and demonstration of rabies virus antigen.