Quantitative determination of morphine in urine has been done by utilizing spectrophotodensitometry.Morphine standard in methanol 5 g/mL was spotted by linomat between 4-24 L in metal sheet thin layerchromatography spectrophotodensito-metry using morphine standard of 20.40.60.80.100.120. and 140 ng.Methode. statistically and validation analysis, showed a regression line of y = -69.21 + 8.06 x with r =0.992. Detection and quantitation limits of 18.02 ng and 60.06 ng respectively were measured at maximum wavelength of (maks) 287 nm with on recovery of 90.91 percent.
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