Jurnal Kimia (Journal of Chemistry)
Vol. 8, No. 1 Januari 2014

KEMAMPUAN TANAH HUTAN MANGROVE SEBAGAI SUMBER ENZIM DALAM HIDROLISIS ENZIMATIK SUBSTRAT SEKAM PADI

Ni Luh Md. Widayantini (Unknown)
I Nengah Wirajana (Unknown)
Putu Suarya (Unknown)



Article Info

Publish Date
01 Jan 2014

Abstract

Cellulase activity of mangrove forest soil from Suwung Kauh Beach in Bali has been reported in previous study. An enzymatic hydrolysis of rice husk with this mangrove forest soil as a source of enzymes was conducted. The aim of this study was to determine the ability of this mangrove forest soil as a source enzymes in hydrolyzing rice husk with and without delignification incubated in varied durations. The rice husk with and without delignification were mixed with mangrove forest soil  and incubated at pH 7 and  29oC with a various incubation times of 0, 1, 2 , 3, and 4 weeks. Reducing sugar content of the results of incubation was measured by spectrophotometry using the Nelson-Somogyi method. The results of this study showed that the mangrove forest soil can hydrolyse the delignification rice husk, but could not degrade the rice husk without delignification. The highest reducing sugar content of 0,892 mg/100mL was resulted from hydrolysis of the delignification rice husk during one week. This result indicated that the mangrove forest soil as a source of cellulase had an ability in hydrolyzing the delignificated rice husk in pH 7 and 29oC incubated in one week.

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Journal Info

Abbrev

jchem

Publisher

Subject

Chemistry

Description

Jurnal Kimia (Journal of Chemistry) publishes papers on all aspects of fundamental and applied chemistry. The journal is naturally broad in scope, welcomes submissions from across a range of disciplines, and reports both theoretical and experimental ...