Jurnal Kimia (Journal of Chemistry)
Vol. 3, No. 1 Januari 2009

ISOLASI DAN IDENTIFIKASI SENYAWA ISOFLAVON DARI KACANG KEDELAI (Glycine max)

I. A. R. Astiti Asih (Unknown)



Article Info

Publish Date
13 Nov 2012

Abstract

Isolation and identification of flavonoid compound from soy bean (Glycine max) have been conducted inthis research. Extraction of 1740.83 g of soy bean powder with methanol yielded 17.82 g yellowish brown of crudemethanol extract. The crude extract was then hydrolysed with 2N HCl. Following partition of hydrolysed productwith n-hexane gave 2.61g of n-hexane extract. Phytochemical testing of n-hexane extract gave positive result forflavonoid compound. The n-hexane extract was then subjected to column chromatography for purification.Identification of fraction which contained flavonoid using UV-Vis showed two band peaks at 312.9 nm dan 268.2nm. Further identification using chemical shift including sodium hydroxide, sodium acetate, sodium acetate:borateacid, aluminium chloride, aluminium chloride-concentrated chloride acid suggested that the compound isolated wasisoflavone without any free hydroxyl group and ortho dihydroxy group at ring A. Thr infra red spectra of isolateindicated the presence of characteristic functional group such as aromatic CH, aliphatic C-H, C=O, aromatic C=C,and C-O

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Journal Info

Abbrev

jchem

Publisher

Subject

Chemistry

Description

Jurnal Kimia (Journal of Chemistry) publishes papers on all aspects of fundamental and applied chemistry. The journal is naturally broad in scope, welcomes submissions from across a range of disciplines, and reports both theoretical and experimental ...