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Developing biochemical teaching material based on research results of amino acid compound identification of seaweed (Dictyota patens) in the Segara beach Sanur Brahmana, Eti Meirina; Astitiasih, I A Raka
Jurnal Pendidikan Biologi Indonesia Vol 3, No 2 (2017): JULY
Publisher : University of Muhammadiyah Malang

This study aims to identify the content of amino acids in seaweed (Dictyota patens) in Segara Sanur Beach which then developing teaching materials based on the results obtained. The method used to test the amino acids was HPLC, meanwhile the data collection has been done by employing questionnaires. The normality test was measured by using normality test, besides the homogeneity test and Paired Sample Test were also used to analyze the homogeneity and significant difference of the data gained. The development of teaching materials has been conducted through several stages namely: needs analysis, design of teaching materials, development of teaching materials, validation of teaching materials by experts, and experiments of teaching materials. The amino acid contents were done by using HPLC. The amino acids contained (% w/w) in the seaweed are aspartic acid (0.36), glutamate acid (0.57), serine (0.20), histidine (0.06), glycine (0.25), threonine (0.17), arginine (0.59), alanine (0.08), tyrosine (0.02), methionine (0.12), valine (0.22), phenylalanine (0.14), Isoleucine (0.16), leucine (0.33) and lysine (0.93). While in the development of teaching materials there is an increase in student learning outcomes by 0.28 with good criteria and there is a significant difference in students learning outcomes between the experimental class and control. Student response is 82.07% which means very strong toward the use of teaching materials.

Asupan Glikosida Flavonoid Terong Belanda (Solanum betaceum Cav.) Terhadap Aktivitas Superoksida Dismutase Dan Kadar Malondialdehid Tikus Wistar Yang Diberi Aktivitas Fisik Maksimal Jeane, Magda; Astiti Asih, Ida Ayu Raka; Bogoriani, Ni Wayan
Jurnal Media Sains Vol 2, No 1 (2018): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

ABSTRAKTujuan dari penelitian ini adalah untuk mengetahui pengaruh asupan glikosida flavonoid terong belanda (Solanum betaceum Cav.) terhadap aktivitas Superoksida Dismutase (SOD) darah tikus Wistar yang diberi aktivitas fisik maksimal. Sampel penelitian adalah 24 ekor tikus yang dibagi kedalam 4 kelompok perlakuan yaitu: kontrol negatif, kontrol positif, pemberian ekstrak etanol, dan pemberian fraksi n-butanol. Aktivitas SOD diukur menggunakan kit Biovision. Hasil penelitian menunjukkan bahwa perlakuan yang diberikan memberikan perbedaan yang signifikan (p<0,05) terhadap aktivitas SOD. Pemberian glikosida flavonoid fraksi n-butanol meningkatkan aktivitas SOD tikus Wistar yang diberikan aktivitas fisik maksimal sebanyak 22,79% lebih tinggi dibandingkan pemberian asupan ekstrak etanol terong belanda. Keyword : Solanum betaceum Cav., Superoksida Dismutase, Aktivitas fisik, stres oksidatifABSTRACTThis study was conducted to determine the effect of flavonoid glycoside of Tamarillo (Solanum betaceum Cav.) on the activity of Wistar rat?s blood Superoxide Dismutase (SOD) enzyme provided maximum physical activity. Twenty four rats divided into 4 groups; negative control, positive control, ethanol extract, and n-butanol fraction. Superoxide Dismutase Enzyme activity was measured using Biovision kit. Results showed that the treatments gave significant difference (p<0.05) to SOD. Flavonoid glycoside from n-butanol fraction increased SOD activity of Wistar rat 22,79% higher than ethanol extract. Keyword : Solanum betaceum Cav., Superoxide Dismutase, Physical activity, oxidative stress

UJI KANDUNGAN ASAM AMINO PADA RUMPUT LAUT Dictyota patens DI PANTAI SEGARA SANUR I A Raka Astitiasih, Eti Meirina Brahmana
Menara Ilmu Vol 11, No 75 (2017): Vol. XI Jilid 1 No. 75, April 2017
Publisher : LPPM Universitas Muhammadiyah Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33559/mi.v11i75.142

Telah dilakukan uji kandungan asam amino pada rumput laut Dictyota patens di Pantai Segara Sanur. Pengujian kandungan asam amino menggunakan HPLC (High Perfomance Liquid Chromatografhy). Asam amino yang terkandung (% b/b) dalam rumput laut tersebut adalah asam aspartat (0,36), asam glutamate (0,57), serin (0,20), histidin (0,06), glisin (0,25), treonin (0,17), arginin (0,59), alanin (0,08), tirosin (0,02), metionin (0,12), valin (0,22), fenilalanin (0,14), isoleusin (0,16), leusin (0,33) dan lisin (0,93).Â Keywords: Asam Amino, Dictyota patens, Pantai Segara Sanur

Antibacterial Activity of Flavonoids from Ethyl Acetate Extract of Milk Banana Peel (Musa x paradisiaca L.) Wiwik Susanah Rita; Ida Ayu Raka Astiti Asih; I Made Dira Swantara; Ni Luh Yuli Damayanti
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.223

Infectious diseases caused by Staphylococcus aureus and Escherichia coli can be treated by milk banana peels (Musa × paradisiaca L.). This study aims to reveal the antibacterial activity of milk banana peel ethyl acetate extract against Staphylococcus aureus and Escherichia coli, and to identify their active compounds. Extraction was conducted by maceration at room temperature, followed by partition. Separation was carried out by column chromatography with mobile phase of chloroform: ethyl acetate (7.5:2.5) and stationary phase of silica gel 60. Antibacterial assay was performed by well diffusion method and identification of active compounds was analyzed by UV-Vis spectrophotometry and LC-MS/MS. Extraction of the peel powder produced methanol extract, partition of the methanol extract resulted n-hexane, ethyl acetate, n-butanol, and water fractions. Antibacterial activity assay shows that ethyl acetate extract was the most active to inhibit the growth of both bacteria. Separation with column chromatography resulted 5 fractions. Identification of the most active fraction with UV-Vis showed that the isolate gave maximum absorption at λ 339.00 nm (band I) and λ 262.00 nm (band II) which were thought to be flavonol (3-OH substituted). Analysis with LC-MS/MS shows that the most active fraction contained 3-methacrylate flavonols and 3-(furan- 2yl) acrylate flavonols.

Sintesis dan Karakterisasi Grafena dengan Metode Reduksi Grafit Oksida Menggunakan Pereduksi Zn Yudha Taufantri; Irdhawati Irdhawati; Ida Ayu Raka Astiti Asih
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 2, No. 1, Mei 2016
Publisher : Syarif Hidayatullah State Islamic University

Graphene is a thin material, has a hexagonal two-dimentional lattice and is considered as an interesting material for adsorption process. Nowadays, graphene has been known as a potential material for diverse application, such as adsorbent. In this study graphene was synthesized from graphite. Furthermore, graphene was applied for adsorption of dichloro diphenyl trichloroethane (DDT). Graphene was synthesized by Hummer’s method using hydrothermal and reduced by Zn. The samples were characterized by Scanning Electron Microscope (SEM) and X-Ray Diffraction (XRD) methods. The results of the XRD showed graphene structure in the 2θ, appeared at 23.9369 with interlayer spacing was about 3.71763 Å, compared with graphite oxide structure in the 2θ appeared at 11.2055 with interlayer spacing was about 7.89649 Å. The results of SEM analysis showed graphene has one layer with planar hexagonal structure and seems transparent whose single layer and multi layers. The graphene adsorption was analyzed by using the UV-Visible spectrophotometer. The results indicated the surface area of graphene was shown as 46.8563 m2/g. The amount of DDT adsorbed by graphene during 15 minutes was 7.5859 mg/g. This adsorption mechanism of DDT and graphene might be due to π-π and hydrogen interactions. Keywords: Adsorption, dichloro diphenyl trichloroethane (DDT), graphena. DOI: http://dx.doi.org/10.15408/jkv.v2i1.2233

Developing biochemical teaching material based on research results of amino acid compound identification of seaweed (Dictyota patens) in the Segara beach Sanur Eti Meirina Brahmana; I A Raka Astitiasih
JPBI (Jurnal Pendidikan Biologi Indonesia) Vol. 3 No. 2 (2017): JULY
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jpbi.v3i2.4036

This study aims to identify the content of amino acids in seaweed (Dictyota patens) in Segara Sanur Beach which then developing teaching materials based on the results obtained. The method used to test the amino acids was HPLC, meanwhile the data collection has been done by employing questionnaires. The normality test was measured by using normality test, besides the homogeneity test and Paired Sample Test were also used to analyze the homogeneity and significant difference of the data gained. The development of teaching materials has been conducted through several stages namely: needs analysis, design of teaching materials, development of teaching materials, validation of teaching materials by experts, and experiments of teaching materials. The amino acid contents were done by using HPLC. The amino acids contained (% w/w) in the seaweed are aspartic acid (0.36), glutamate acid (0.57), serine (0.20), histidine (0.06), glycine (0.25), threonine (0.17), arginine (0.59), alanine (0.08), tyrosine (0.02), methionine (0.12), valine (0.22), phenylalanine (0.14), Isoleucine (0.16), leucine (0.33) and lysine (0.93). While in the development of teaching materials there is an increase in student learning outcomes by 0.28 with good criteria and there is a significant difference in students learning outcomes between the experimental class and control. Student response is 82.07% which means very strong toward the use of teaching materials.

AKTIFITAS ANTIINFLAMASI TOPIKAL MINYAK ATSIRI DAN EKSTRAK ETER TUMBUHAN TENGGULUN, PROTIUM JAVANICUM, BURM TERHADAP MODEL INFLAMASI KULIT PADA TIKUS I Wayan Suirta; Ni Made Puspawati; I.A. Raka Astiti Asih
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 4 No 1 (2016)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.242 KB)

ABSTRAK: Tenggulun (Protium javanicum, Burm ) secara tradisional telah dimanfaatkan masyarakat Bali sebagai tanaman obat untuk mengobati bengkak. Penelitian ini bertujuan untuk menentukan aktivitas antiinflamasi ekstrak eter kulit batang dan minyak atsiri daun tengulun pada udem (inflamasi) telinga tikus yang diinduksi TPA (12-O-tetradecanoylphorbol-13-acetate) secara topikal. Ekstraksi dilakukan dengan maserasi dan isolasi minyak atsiri dengan distilasi uap. Pada penelitian ini digunakan rancangan acak lengkap dengan sembilan kelompok perlakuan dengan masing-masing perlakuan terdiri dari lima ekor tikus. Inflamasi diukur 6 jam setelah perlakuan. Hasil triturasi 180g ekstrak etanol kulit batang tenggulun diperoleh 6,30 g ekstrak dietil eter dan hasil distilasi uap 4,5 kg daun segar diperoleh 2,5 g minyak atsiri. Hasil uji fitokimia ekstrak kulit batang tenggulun mengandung senyawa golongan terpenoid dan minyak atsiri mengandung senyawa flavonoid, terpenoid, dan steroid. Hasil uji aktifitas antiinflamasi menunjukkan bahwa persentase hambatan peradangan dari ekstrak tenggulun didapatkan : KEI (58,34%) , KEII (54,17%) , KEIII (45,85%) dan KAI (50,02%) yang menunjukkan hambatan yang tidak berbeda secara bermakna dengan hambatan yang diberikan oleh kontrol positip KP (62,50%). Ekstrak eter 12 mg dan 20 mg masih memberikan hambatan peradangan yang lebih besar dari minyak atsiri 20 mg. Hasil uji histologi menunjukkan ekstrak eter dan minyak atsiri dapat menghambat migrasi sel radang lebih dari 50%. Jumlah sel radang yang bermigrasi ke daerah peradangan sebanyak 17 sel untuk ekstrak eter dosis 20 mg/telinga dan 20 sel radang untuk minyak atsiri dosis 20 mg/ telinga. Dengan induksi TPA jumlah sel radang yang bermigrasi ke daerah peradangan sebanyak 64 selABSTRACT: Protium javanicum, Burm (Tenggulun) has been used traditionally in Bali as medicines to treat inflamation. This research aimed to evaluate topical anti-inflammatory activity of stem bark ether extract and essential oil of leaves on ear edema rats induced by TPA (12-O-tetradecanoylphorbol-13-acetate). Extraction of stem bark was done by maceration and the essential oil was produced by steam distillation. Complete Randomized Design with nine treatment groups was used in this study. Each of the treatment had five repetitions. The inflammation was measured after 6 hours of treatment. Trituration of 180 g crude ethanol extract with ether gave 6.30 g ether extract and steam distillation of 4.5 kg fresh leaves yielded 2.5 g of the essential oil. Phytochemical study showed that stem bark ether extract only contained triterpenoid while the essential oil of leaves contained flavonoid, terpenoid, and steroid compounds. The yield of % inhibited antiinflamatory activity showed that KEI (58,34%), KEII (54,17%), KEIII (45,85%) and KAI (50,02%) was insignificantly different to positive control dexamethason which inhibited inflammation by 62.50%. The result of histology test showed that ether extracts and essential oils can inhibit the formation of inflammatory cells of more than 50%. Dosis of 20 mg/ear of ether extract (formed 17 inflammatory cells) and 20 mg/ear of essential oil (formed 20 inflammatory cells) can inhibit the formation of inflammatory cells induced by TPA (formed 64 of inflammatory cells).

AKTIVITAS ANTIOKSIDAN SENYAWA FLAVONOID EKSTRAK ETANOL BIJI TERONG BELANDA (Solanum betaceum, syn) DALAM MENGHAMBAT REAKSI PEROKSIDASI LEMAK PADA PLASMA DARAH TIKUS WISTAR Ni Wayan Oktarini A.C.Dewi; Ni Made Puspawati; I Made Dira Swantara; Ida Ayu Raka Astiti Asih; Wiwik Susana Rita
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 2 No 1 (2014)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK : Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dari biji terong belanda dalam menghambat reaksi peroksidasi lemak plasma darah pada tikus dan menentukan golongan flavonoid yang aktif sebagai antioksidan. Uji aktivitas antioksidan dilakukan dengan metode DPPH dan pengukuran kadar MDA darah tikus Wistar. Partisi ekstrak etanol biji menghasilkan fraksi n-heksan, etil asetat, dan n-butanol. Fraksi etil asetat dan n-butanol positif mengandung senyawa flavonoid. Uji aktivitas antioksidan menggunakan metode DPPH menunjukkan bahwa fraksi etil asetat memiliki aktivitas antioksidan lebih besar dengan nilai IC50 1162,608 ppm. Fraksi etil asetat dipisahkan dengan kromatografi kolom menggunakan eluen n-heksan:etil asetat:n-butanol (6:4:0,1), diperoleh dua fraksi (Fraksi A dan fraksi B). Analisis dengan spektroskopi inframerah menunjukkan bahwa kedua isolat diduga mengandung gugus fungsi yang sama (OH, CH, C=O, C=C aromatik, C-O, CH alifatik. Analisis dengan spektroskopi UV-Vis diindikasikan bahwa fraksi A merupakan golongan dihidroflavonol dengan gugus hidroksi pada cincin A yaitu pada atom C-6, C-7 atau C-7, C-8 dan fraksi B merupakan golongan flavanon dengan gugus hidroksi pada cincin A yaitu pada atol C-7 dan C-8. Hasil analisis statistik pada pengukuran kadar MDA darah tikus Wistar menunjukkan bahwa fraksi etil asetat dengan dosis 200 mg/kgBB mampu menurunkan kadar MDA darah tikus Wistar yang diberi aktivitas fisik maksimal. Kata kunci : Solanum betaceum, syn, malondialdehid, peroksidasi lemak, flavonoid ABSTRACT : The aims of this study are to determine the antioxidant activity of flavonoid compounds extracted from Dutch eggplant seeds in inhibiting lipid peroxidation reactions in the Wistar rat blood plasma and to determine the active flavonoid compounds as antioxidants. The test of antioxidant activity was carried out with DPPH (diphenilpikril hidrazil) method and measurment of MDA (malondialdehyde) level of blood of the Wistar rats. The ethanol extract with partitioned with n-hexane, ethyl acetat, and n-butanol. Fractions of ethyl acetate and n-butanol positively contain flavonoids. The test of antioxidant activity by DPPH method showed that the ethyl acetate fraction had strong antioxidant activity with IC50 value of 1162.608 ppm. Further, ethyl acetate fraction was separated by column chromatography using the mixture of n – hexane, ethyl acetate, water eluent ( 6:4:0,1 ) and it was obtained two fractions (named as fraction A and B). The analysis by infrared spectroscopy showed that the two isolates were suspected to be having similar functional groups (OH, CH, C=O, C=C aromatic, C-O and CH aliphatic). Analysis by UV-Vis spectroscopy indicated that fraction A was dihydroflavonol containing hydroxyl group on ring A located at C-6 and C-7 or C-7 and C-8 while the fraction B was flavanon containing hydroxyl group on ring A located at of C-7 and C-8. Statistic analysis of MDA level of the Wistar rat blood plasma showed a significant effect on MDA level where the dose of 200 mg/kgBW of the ethyl acetate fraction could decrease the MDA level of the Wistar rats blood plasma after they were forced to do maximum physical activities.

AKTIVITAS ANTIBAKTERI EKSTRAK KULIT PISANG (Musa sp.) TERHADAP Escherichiacoli DAN Staphylococcus aureus SERTA IDENTIFIKASI GOLONGAN SENYAWA AKTIFNYA Ida Ayu Raka Astiti Asih; Wiwik Susanah Rita; I Gusti Bagus Teguh Ananta; Ni Kadek Dyan Mustika Sri Wahyuni
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 6 No 1 (2018): Volume 6, Nomor 1, 2018
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Kulit pisang (Musa sp.) dapat digunakan sebagai bahan penghambat bakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kulit pisang (Musa sp.) terhadap Escherichia coli dan Staphylococcus aureus serta identifikasi senyawa aktifnya.. Ekstraksi dilakukan dengan teknik maserasi dengan etanol, selanjutnya ekstrak etanol dipartisi berturut-turut dengan n-heksana, etilasetat, dan n-butanol. Ekstrak n-heksana, etil asetat, dan n-butanol, yang diperoleh diuji fitokimia dan aktivitas penghambatan terhadap bakteri Escherechia coli dan Staphylococcus aureus. Uji penghambatan bakteri dilakukan dengan metode sumur difusi pada media Nutrient Agar (NA).Ekstraksi 500 g kulit pisang (Emas, Kepok, Susu dan Kayu) masing-masing menghasilkan 15, 20, 10, 12 gram ekstrak pekat yang berwarna kuning. Hasil uji fitokimia menunjukkan bahwa ekstrak kulit pisang mengandung senyawa alkaloid, terpenoid, fenol dan flavonoid. Hasilujiaktivitas terhadap bakteri Escherechia coli dan Staphylococcus aureus menunjukkan bahwa ekstrak kulit pisang Mas dan Kepok adalah ekstrak yang aktif dengan MIC masing masing 1% dan 0,5%, untuk kulit pisang mas dan 0,5% serta 0,1%, untuk kulit pisang kepok. Kata Kunci: Antibakteri, Musa sp., Escherichia coli, Staphylococcus aureus, Minimum Inhibitory Concentration (MIC) ABSTRACT: The banana peel (Musa sp.) can be used as antibacteria. This study aims to determine the antibacterial activity of banana peel (Musa sp.) against Escherichia coli and Staphylococcus aureus and to identify their active compounds. Extraction was done by maceration technique with ethanol, then ethanol extract was partitioned successively with n-hexane, ethylacetate, and n –butanol to get n-hexane, ethyl acetate, and n-butanol extract. The extract was tested phytochemical and activity of against Escherichia coli and Staphylococcus aureus. The antibacterial activity test was performed by diffusion well method on Nutrient Agar (NA) medium. Extraction of 500 g banana peel (Mas, Kepok, Susu and Kayu) each produced 15, 20, 10, 12 grams of yellow-colored concentrated extract. The phytochemical test results showed that banana peel extract contains alkaloids, terpenoids, phenols and flavonoids. The antibacterial activity test of the banana peels against Escherechia coli and Staphylococcus aureus showed that banana extract of Mas and Kepok was the active extract with MIC 1% and 0.5%, for mas and 0.5% and 0,1%, for kepok peel.

ADSORPSI Cr(III) PADA SILIKA GEL TERMODIFIKASI DIFENILKARBAZON SECARA SOL-GEL Ida Ayu Raka Astiti Asih; I Wayan Sudiarta
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 4 No 1 (2016)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK : Telah dilakukan penelitian mengenai pemanfaatan silika gel termodifikasi difenilkarbazon (Si-CPTMS-DPZon) secara sol-gel untuk mengadsorpsi ion logam kromium(III). Studi adsorpsi dilakukan dengan metode Batch. Pengaruh waktu dan pH awal larutan kromium dipelajari untuk mendapatkan kondisi optimum adsorpsi dan variasi konsentrasi ion kromium digunakan untuk mendapatkan kapasitas adsorpsi. Model isotermal Langmuir dan Freundlich digunakan untuk menentukan isoterm adsorpsi. Hasil penelitian menunjukkan bahwa adsorpsi Cr(III) pada adsorben Si-CPTMS-DPZon optimum terjadi pada pH 4 dan waktu interaksi 90 menit, dengan kapasitas adsorpsi sebesar 36,28 mg/g dan energi adsorpsi sebesar 29,16 kJ/mol. Adsorpsi yang terjadi lebih cenderung mengikuti isoterm Langmuir dengan nilai R2 sebesar 0,9971 dibanding dengan isoterm Freundlich dengan R2 0,5612. Adsorpsi Cr(III) pada adsorben dominan melalui adsorpsi kimia. ABSTRACT : Research about utilizing of diphenylcarbazone-modified silica gel prepared by sol-gel method (Si-CPTMS-DPZon) to adsorp chromium (III) ions has been carried out. This study was conducted using batch adsorption. The influence of time and initial pH of chromium solution were studied to obtain the optimum adsorption conditions, and the variation of the concentration of chromium ion was used to obtain the adsorption capacity. Langmuir and Freundlich isotherm models were used to determine the model of isotherm adsorption. The results showed that the optimum adsorption of Cr (III) on the adsorbent Si-CPTMS-DPZ occurred at pH 4 with 90 minutes of contact time. The adsorption capacity of Cr(III) on Si-CPTMS-DPZ was 36.28 mg/g with the adsorption energy of 29.16 kJ/mol. The adsorption profile tends to follow the Langmuir isotherm with R2 values of 0.9971 rather than the Freundlich isotherm with R2 of 0.5612. Adsorption of Cr (III) on the adsorbent dominantly occurred through chemical adsorption.

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