IDENTIFIKASI SENYAWA TOKSIK EKSTRAK KLOROFORM KULIT BIJI NYAMPLUNG (Calophyllum inophyllum L.)

Sri Rahayu Santi
I Wayan Suirta
Kadek Agus Andika Pratama

Jurnal Kimia (Journal of Chemistry)
Vol. 6, No. 2 Juli 2012

Identification  of active toxic compounds from chloroform extract Nyamplung skin bean (Calophyllum inophyllum L) have been conducted. As much as 2,85 g n-hexane extract, 7,42 chloroform extract, and 4,71 water extract were resulted from 1 kg Nyamplung skin bean. Separation of  2 g  chloroform extract  using column chromatography (stationary phase: silica gel 60 and mobile phase chloroform-methanol 9,5:0,5) resulted  in four fraction with yellowish fraction (0,473 g) as the most toxic fraction (LC50 8,241 ppm). Identification using GC-MS showed that the isolate was hexadecanoic acid (21,02%), 8-octadecenoic acid (59,55%), and oktadecanoic acid(19,43%).