Kadek Agus Andika Pratama
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IDENTIFIKASI SENYAWA TOKSIK EKSTRAK KLOROFORM KULIT BIJI NYAMPLUNG (Calophyllum inophyllum L.) Sri Rahayu Santi; I Wayan Suirta; Kadek Agus Andika Pratama
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 2 Juli 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Identification  of active toxic compounds from chloroform extract Nyamplung skin bean (Calophyllum inophyllum L) have been conducted. As much as 2,85 g n-hexane extract, 7,42 chloroform extract, and 4,71 water extract were resulted from 1 kg Nyamplung skin bean. Separation of  2 g  chloroform extract  using column chromatography (stationary phase: silica gel 60 and mobile phase chloroform-methanol 9,5:0,5) resulted  in four fraction with yellowish fraction (0,473 g) as the most toxic fraction (LC50 8,241 ppm). Identification using GC-MS showed that the isolate was hexadecanoic acid (21,02%), 8-octadecenoic acid (59,55%), and oktadecanoic acid(19,43%).