International Journal of Biosciences and Biotechnology
Vol 5 No 2 (2018)

IN VIVO MULTISTEP MUTAGENESIS INDUCTION USING COLCHICINE ON COWPEA MUTANT 1 (Vigna unguiculata L. Walp)

I Gede Ketut Susrama (Department of Agroecotechnology, Faculty of Agriculture, Udayana University)
I Gede Putu Wirawan (Department of Agroecotechnology, Faculty of Agriculture, Udayana University)



Article Info

Publish Date
21 May 2018

Abstract

In the previous research, we obtained three catagories of M1 mutant cowpea namely 1) purple pod mutant cowpea, 2) green pod mutant cowpea which has three pods in a stalk, and 3) green pod mutant cowpea which has two pods in a stalk. All those three catagories of M1 mutant cowpeas were treated again with colchicine in a multistep mutagenesis proses in vivo. Then, we found changes in number of leaflet in a petiole to 4 leaflets (quadrifoliate) and to five leaflets in a petiole (pentafoliate). These changes are indications that beside as a chromosome multipying alkaloid, colchicine causes other genetic changes as well. Through this mutagenesis research, we created gigas mutant cowpea that having longer leaves, longer pods and heavier seeds. The accurrence of two kinds of insect pests attack were found namely cowpea aphid Aphis craccivora and pod sucking bug Riptortus linearis.

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Journal Info

Abbrev

jbb

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Environmental Science

Description

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of ...