Jurnal Ilmu dan Kesehatan Hewan
Vol. 1 No. 1 2013

Polimorfisme Lokus Mikrosatelit D10S1432 pada Populasi Monyet Ekor Panjang Di Sangeh

F. Rell (FKH UNUD Denpasar)
S.K. Widyastuti (FKH UNUD Denpasar)
I N. Wandia (FKH UNUD Denpasar)



Article Info

Publish Date
26 Feb 2013

Abstract

Genetic polymorphisms are variations in the genetic structure of populations that represents the base level of biodiversities.  This research aimed to assess the polymorphism of microsatellite locus of D10S1432 in the population of long-tailed macaques in Sangeh, Bali.  A total of 18 blood samples collected as a source of DNA. DNA was extracted using QIAmp® DNA Blood Mini Kit. Microsatellite locus was amplified by polymerase chain reaction technique (PCR) with a total of 30 cycles, and a 57oC of annealing temperature. Furthermore, the alleles were separated by 7% polyacrylamide gel electrophoresis, and emerged with silver staining. The results showed that there were three types of alleles found at D10S1432 locus in the long-tailed macaques population in Sangeh. The alleles size varied from 170 bp to 186 bp. The allele frequencies were  0.28, 0.67, and 0.05 for allele 170, allele 174, and allele 186 consecutively. The heterozygosity of the locus was 0.48. It can be concluded that microsatellites locus D10S1432 is polymorphic in the long-tailed macaques population in Sangeh.

Copyrights © 2013






Journal Info

Abbrev

jikh

Publisher

Subject

Health Professions Veterinary

Description

Jurnal Ilmu dan Kesehatan Hewan menerima naskah ilmiah original (belum pernah dipublikasikan) dalam bidang veteriner atau yang terkait dengannya. Jurnal Ilmu dan Kesehatan Hewan dikelola oleh Program Studi Magister Kedokteran Hewan Universitas Udayana dan terbit dua kali ...