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Jurnal Ilmu dan Kesehatan Hewan
Published by Universitas Udayana
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Core Subject : Health,
Jurnal Ilmu dan Kesehatan Hewan menerima naskah ilmiah original (belum pernah dipublikasikan) dalam bidang veteriner atau yang terkait dengannya. Jurnal Ilmu dan Kesehatan Hewan dikelola oleh Program Studi Magister Kedokteran Hewan Universitas Udayana dan terbit dua kali setahun.
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Articles 45 Documents
Asosiasi Polimorfisme Mikrosatelit DRBP1 Gen BoLa (Bovine Leucocyte Antigen) dengan Ukuran Tubuh pada Sapi Bali Ni Wayan Patmawati; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Genomic selection has a potency for improving the genetic quality of livestock. In effort to find out a molecular marker or DNA segment which is linked with a certain phenotype, we examined the association of the polymorphism of microsatellite DRBP1 with the body size of Bali cattle. A total of 55 blood samples of sires was collected from the Breeding Center of Bali Cattle Improvement Office in District of Jembrana. Total DNA was extracted with QIAamp DNA blood mini kit. The locus was amplified using PCR technique and alleles were separated through PAGE 6%, and visualized with silver staining. We found 5 alleles which their length varied from 108 bp to 136 bp. Allele 122 had the highest frequencies (25.45%). The expected heterozygositiy (HE) and observed heterizygosities (HE), and the PIC were 0.783, 0.891 and 0.738 consecutively. Statistic analysis showed that the polymorphism of microsatellite DRBP1 had no significant association (p > 0.05) with body size of male Bali cattle. This indicates that the DRBP1 does not play a role in determining the body size.
Crude Antigen Cystisercus Taenia Saginata Isolat Bali untuk Deteksi Sistiserkosis pada Sapi Hertati Anriani Lubis; I Made Damriyasa; Nyoman Sadra Dharmawan
Veterinary Science and Medicine Journal Vol 2 No 1 (2014)
Publisher : Udayana University

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Abstract

The purpose of this study was to evaluate Taenia saginata cystisercus antigen for the detection of bovine cysticercosis. Taenia saginata cysticercus antigen was derived from local isolates, obtained from the experimental infection of Taenia saginata tape worms from Bali. The research was done by ELISA (Enzyme Linked Immunosorbent Assay) optimized by determining the optimal concentration of antigen, the optimal dilutions of serum and the optimal dilutions of conjugate. The results showed that Taenia saginata cysticercus crude antigen (Bali isolate) are antigenic and can be used to detect cattle cysticercosis. Optimal concentration of antigen: 2 ug/ml, optimal dilutions of serum: 1:80 and optimal dilution of conjugate: 1:4000.
Risk Factors and Spatial Distribution of Rabies in Bali in the Year of 2014 IGNA Wisnu Adi Saputra; I Nyoman Dibia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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Abstract

The aims of this study was to determine the spatial distribution and the risk factors associated with theincidence of rabies in Bali on the year of 2014. A total of 1286 brain samples from all over the Bali wasexamined.  Out of  all brain samples, 130 samples were diagnosed positive for rabies. Based on these data,we collected the dogs location coordinates using a tool of Global Positioning System (GPS). Thosecoordinates  were, then, analyzed with GIS software. The results showed that spatial distribution of theincidence of rabies in each regencies in Bali varied, however, it had a same pattern. The factors ofcounseling, breed, trade, and geographical condition associated to the incidence of rabies. Further researchis needed to map the incidence of rabies over a longer time (more than 3 years), to improve theeffectiveness of surveillance in connection with the eradication of rabies in Bali.
Resistensi Bakteri Aeromonas sp. Isolat Ikan Piranha (Pygosentrus nattereri) Bali Safari And Marine Park terhadap Antibiotik Ni Made Herawati; Hapsari Mahatmi; I Nengah Kerta Besung
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

Piranha (Pygosentrus nattereri) is a fish that has been conserved at Conservation Center of Bali Safari and Marine Park. In May 2012 there was a case that twelve Piranhas suffered from illness and two of them died without any specific clinical signs. The dead Piranhas were necropsied and the tissues or parts of organs were collected for microbial examination interests. Spesies specific bacterial examination was also included using Microgen GNA TM + B-ID system test The bacterial examination found that the fishes were infected by Aeromonas hydropila and Aeromonas veronii. Further examination about antibiotic resistance tests showed that those Aeromonas spp were resistant to chloramphenicol and a combination of trimethoprim and sulphamethoxazole. It can be suggested that, next, the usage of other antibiotics is strongly recommended for the case of Piranha infected by Aeromonas spp at Bali Safari and Marine Park.
Elektrokardiogram Monyet Ekor Panjang (Macaca fascicularis) yang Teranestesi Kombinasi Ketamin-Silasin Deny Rahmadani; Putu Gede Yudhi Arjentinia; I Gede Soma
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

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Abstract

Electrocardiograph principally is an electrical activity of heart muscle, which initiated and started by thesinoatrial node in right atrium, from which it spreads out through the all of the heart muscle. Centralnervous system influenced the strength and frequency of electrical impulse. In the anesthetized animal,heart contraction does not influenced by central nervous system. The study aimed to observe theelectrocardiogram (ECG) pattern of the wild Macaca fascicularis anesthetized by ketamine and xylazinecombination. The ECG pattern were recorded on 10 wild Macaca fascicularis during anesthetic period. Thedata were analyzed using descriptive method. The result showed that the average of the P wave was 0.04second with amplitude up to 0.10 mV; QRS complex 0.08 second and 0.36 mV; PR interval 0.13 second;QT interval 0.27 second; RR interval 14.2 mm; PR segment 0.04 second; ST segment 0.07 second;amplitude of T wave  up to 0.19 mV. All of the monkeys have sinus rhythmic with average heart rate is 106beats per minute.
Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed Macaque Populations in Bali Island, Indonesia I Nengah Wandia; I Gede Soma; I Gusti Agung Arta Putra
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

The long tailed macaques inhabit throughout Bali Island, however, recently they have been fragmented into many smaller local populations. In spite of that, there are just few reports that describe the mode of fragmentation and the genetic structure of the population. This research aimed to explore the polymorphism of microsatellite loci on Y chromosome in long tailed macaque populations in Bali Island using three human microsatellite primers, namely DYS390, DYS391, and DYS393. A total of 99 blood samples were collected from male long tailed macaques originated from 8 populations (23 from Pulaki, 11 from Bedugul, 13 from Mekori, 8 from Sangeh, 6 from Uluwatu, 11 from Alas Kedaton, 11 from Ubud, and 16 from Bukit Gumang). Total DNA was extracted using QIAamp® Blood Mini Kit. The polymorphism of the microsatellite loci was detected using PCR technique, and allelic variations were separated through 7% poly acrylamide gel electrophoresis. The result of the research showed that DYS390 and DYS393 loci were monomorphic (each had only one allele). The DYS391 locus had two alleles, therefore, this locus was polymorphic. It could be suggested that DYS391 locus could be used to study genetic variation in male long tailed macaque population in Bali Island.
The Effectivity of Ethanol Extract of Datura Metel L. Seeds as a General Anaesthesia on Kintamani Dogs I Putu Juli Sukariada; I Wayan Sudira; I Gusti Ngurah Sudisma
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

This study aimed to determine the effectivity of ethanol extract of Datura metel L. seeds, as general anaesthesia on Kintamani dogs. A total of 25 dogs was divided into 5 groups, namely: group 1(treated by the combination of xylazin 1 mg/kg BW and ethanol extract of 10% Datura metel L. seeds 10 mg/kg BW), group 2 (treated by the combination of xylazin 1 mg/kg BW and ethanol extract of 10% Datura metel L. seeds 15 mg/kg BW), group 3 (treated by the combination of xylazin 1 mg/kg BW and ethanol extract of 10% Datura metel L. seeds 20 mg/kg BW), group 4 (treated by the combination of xylazin 1 mg/kg BW and ketamine 15 mg/kg BW), and group 5 (treated by the ethanol extract of 10% Datura metel L. seeds 15 mg/kg BW). Induction time, duration and recovery time were recorded, in which these responses were observed by clamping on the tail, ears and interdigital area.  Physiological responses including respiration values, rectal temperature, oxygen saturation (SPO2), the frequency of the heart rate and electrocardiogram were observed using physiographic machine every 5 minutes until the dogs regain their consciousness. The level of ALT and AST was also examined to determine the level of toxicity of ethanol extracts of 10% Datura metel L. seeds. The results showed that group 1 and group 2 had no significant effect on the physiological response changes. However, the group 3 showed the sign of acute toxicity followed by increasing the levels of ALT and AST. The group 4 showed a decreasing physiological response, while group 5 had a similar response to group 2, but without sedation effect. It can be concluded that the ethanol extract of 10% Datura metel L. seeds has a potential as an analgesic and an anaesthetic agent.
Pola Resistensi Salmonella typhi yang Diisolasi dari Ikan Serigala (Hoplias malabaricus) terhadap Antibiotik Waode Santa Monica; Hapsari Mahatmi; Kerta Besung
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

The purpose of this study was to know the resistant pattern of Salmonella typhi (S. typhi) isolated from wolf fish (Hoplias malabaricus) to antibiotics in Bali Safari and Marine Park. S. typhi was isolated from fecal swab collected from the illness fish. The S. typhi was confirmed with MicrogenTM GN A ID test’s method. The antibiotic sensitivity of the S. typhi was tested using streptomycin, gentamicin, penicillin, vancomycin, and chloramphenicol according to the Kirby bouer method. The results showed that the isolate of S. typhi was resistant to penicillin and vancomycin, but sensitive to streptomycin, gentamicin, and chloramphenicol.
The Stability of the Bali’s Rabies Virus Molecular Marker for the Development of Diagnostic Method Putu Dimas Abiyoga; Gusti Ayu Yuniati Kencana; I Nyoman Dibia
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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Abstract

The objectives of this study were to prove that the molecular marker of Bali’s rabies virus is still conserveand to develop a diagnostic method based on molecular marker. Thirty brain samples of dog that had beeninfected by a rabies virus from 2014 and 2015 were used for this research. The sequences of nucleotidewhich were obtained and the sequences of nucleotide accessed in GenBank were analyzed using  MEGA5.2 software. The result provided that the specific amino acid (isoleusin) at position 308 (open readingframe) as a molecular marker of Bali’s rabies virus was still conserve. Fragment of N gene amplified byReverse RT-PCR method with a specifically designed primers showed that every isolate of rabies virus hadit’s own typical band, and could distinguish Bali isolate from the others in Indonesia.
Profil Darah Monyet Ekor Panjang (Macaca fascicularis) Liar di Habitat Alami I Gede Soma; I Nengah Wandia; I GA Artha Putra; Rostiani Silta
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

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Abstract

Study of blood profile of Macaca fascicularis in wild habitat was conducted using 29 blood samples of  adult Macaca fascicularis from Alas Purwo National Park and Baluran National Park, East Java. Blood samples of Ketamine-anaesthetized Macaca fascicularis were taken via femoral vein. Data were divided according to the sex. The result showed that there was sexual dimorphisms of blood profiles of adult Macaca fascicularis. Blood profiles of the adult male were the total erythrocytes count: 4.9 ± 0.4 (x106/µl), hemoglobin titer: 10.9 ± 0.9 g/dl, packed cell volume: 35.5 ± 3.5%, blood sedimentation rate: 1.5 ± 0.2 mm/h, MCH: 20.9 ± 2.1 pg, MCV: 63.8 ± 5.7 fL, MCHC: 28.7 ± 2.5 pg, total leukocytes count: 5777 ± 551.1/µl, limphocytes 23.6 ± 1.6%, neutrophils 57.2 ± 1.7%, eosinophils 9.6 ± 0.5%, and monocytes 9.6 ± 0.6%. Blood profiles of adult female were the total erythrocyte count: 3.9 ± 0.5 (x106/µl), hemoglobin titer: 8.8 ± 1.7 g/dl, packed cell volume: 32.1 ± 4.1%, blood sedimentation rate: 1.3 ± 0.3 mm/h, MCH: 20.5 ± 4.2 pg, MCV: 74.8 ± 9.9 fl, MCHC: 24.1 ± 4.7 pg, total leukocytes count: 5244.4 ± 1017,9/µl, limphocytes: 22.9 ± 1.8%, neutrophils 56.3 ± 1.7%, eosinophils 11.2 ± 0.7%, and monocytes 9.6 ± 0.6%.