Karakteristik Fisikokimia Bakteriosin Asal Bakteri Asam Laktat Enterococcus durans Hasil Isolasi Kolon Sapi Bali

I Wayan Suardana
Hana Kristal Alamanda Septiara
I Nyoman Suarsana

Buletin Veteriner Udayana
Vol. 9 No. 2 Agustus 2017

Lactic acid bacteria can excrete antimicrobial compounds like bacteriocins. The study aimed to find out the characteristic of physic-chemical of bacteriocin producing Enterococcus durans isolate 18A isolated from colon’s bali cattle. The study initiated by Gram staining and catalase test, followed by isolation and purification of bacteriocin. The result of the research showed that bacteriocins of Enterococcus durans isolate 18A as a protein with it’s concentrationis 0,272?g/ml and it does not contain carbohydrate. On the other hand, the bacteriocins was not showed a band while tested on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result showed, the bacteriocin producing Enterococcus durans isolate 18A has antimicrobial activity to Bacillus cereus as 23,88%.