AKTIVITAS BIODESULFURISASI DIBENZOTIOFENA DALAM MODEL MINYAK TETRADEKANA PADA RASIO MINYAK AIR DAN KONSENTRASI RESTING SEL ISOLAT BAKTERI SBJ8

I Gede Krisna Putra Pratama (JURUSAN TEKNOLOGI INDUSTRI PERTANIAN FAKULTAS TEKNOLOGI PERTANIAN UNIVERSITAS UDAYANA)
Ida Bagus Wayan Gunam (JURUSAN TEKNOLOGI INDUSTRI PERTANIAN FAKULTAS TEKNOLOGI PERTANIAN UNIVERSITAS UDAYANA)
G.P. Ganda Putra (JURUSAN TEKNOLOGI INDUSTRI PERTANIAN FAKULTAS TEKNOLOGI PERTANIAN UNIVERSITAS UDAYANA)

JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI
Vol 5 No 1 (2017): Maret

This study aims to determine the best ratio of oil / water and concentration of resting cells and determine the time course of resting cells bacteria isolates SBJ8 to be known the biodesulfurization capability in two-phase media using media mineral salt sulfur free-tetradecane(MSSF-TD). Oil/water ratio used in this study is 1 : 1 , 1 : 2 and 1 : 4 in combination with cell concentration by OD66010, 20, and 30. The study was conducted using 200 mg · l–1dibenzothiophene as a source of sulfur dissolved in tetradecane as a model of petroleum (oil phase). Isolates were incubated for 24 hours at 37°C and the residue analyzed by gass cromathography mass selective (GC-MS). Testing the degradation of resting cells is accomplished by two replications. The results showed that the ratio of oil water 1 : 4 by and cellconcentration OD66030 shows the results of the highest degradation of 84.84% and the time course of 24 hours resting cells show degradation rate dibenzothiophene up to 81.84%. Keywords : Biodesulfurization, dibenzothiophene, tetradecane, resting cells