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DELIGNIFIKASI AMPAS TEBU DENGAN LARUTAN NATRIUM HIDROKSIDA SEBELUM PROSES SAKARAIFIKASI SECARA ENZIMATIS MENGGUNAKAN ENZIM SELULASE KASAR DARI ASPERGILLUS NIGER FNU 6018 Gunam, Ida Bagus Wayan; Wartini, Ni Made; Dewi Anggreni, Anak Agung Made; Suparyana, Pande Made
Teknologi Indonesia Vol 34 (2011)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.048 KB) | DOI: 10.14203/jti.v34iKhusus.36

Abstract

Cellulose, the most abundant renewable resource, has received much attention as potential energy and carbon source for the production of useful products such as glucose, ethanol and fuels. The possibility of converting cellulose in bagasse enzymatically into glucose, after being loosened its complex structure chemically into primary one by using sodium hydroxide was studied. Bagasse was soaked in 6% sodium hydroxide for 12 hours at room temperature. This treatment resulted in loosening some cellulose bundle structure shown by release of lignin and hemicelluloses up to 32.11 and 42.87%, respectively and high water retention value of 15.90 (w/w). In this condition the delignifi ed bagasse could be saccharifi ed by crude cellulase enzym from Aspergillus niger. Saccharifi cation enzimatically of 2 g delignifi ed bagasse at 50oC pH 4,8 during 120 hours produced reducing sugar of 54.47 mg/100 ml.
PENGARUH JENIS DAN JUMLAH PENAMBAHAN GULA PADA KARAKTERISTIK WINE SALAK Wayan Gunam, Ida Bagus; Wrasiati, Luh Putu; Setioko, Wito
Agrotekno Vol. 15, No. 1 Februari 2009
Publisher : Universitas Udayana

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Abstract

The research was aimed to determine the effect of typeand concentration of sugar on the characteristics of producedsalacca wine and to determine the type and concentration ofsugar yielding salacca wines with the best characteristics andpreferred by panelists. Sensory test results showed that themost preferred salaca wine characteristics by panelists wasthose which produced with addition of 25% sucrose sugar(w/v), and the produced wine contained 14.81% of ethanol,2.66% of total sugar, 0.21% of total acid, pH 3.67, 10.5°Brixof total soluble solids, and methanol content was notdetected.
Pemanfaatan Tepung Rumput Laut Gracilaria sp. pada Tempe sebagai Alternatif Pangan Sumber Yodium Monikasari, Ni Nyoman Trisa; Wayan Gunam, Ida Bagus; Wisaniyasa, Ni Wayan
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 16, No 1 (2021): Juni 2021
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v16i1.689

Abstract

Tempe merupakan produk pangan bergizi tinggi yang digemari dan diterima oleh masyarakat luas, tetapi umumnya memiliki kadar yodium yang rendah. Rumput laut yang kandungan yodiumnya cukup tinggi dapat digunakan sebagai bahan fortifikasi yodium pada makanan. Penambahan rumput laut pada tempe diharapkan dapat menjadi alternatif pangan sumber yodium untuk menanggulangi masalah GAKY (Gangguan Akibat Kekurangan Yodium). Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan tepung rumput laut Gracilaria sp. terhadap kadar yodium pada tempe serta mengetahui karakteristik yang dihasilkan. Perlakuan yang dilakukan adalah perbandingan kedelai dan tepung rumput laut 100:0; 97,5:2,5; 95:5; 92,5:7,5; dan 90:10. Perbandingan kedelai dan tepung rumput laut 92,5:7,5 merupakan komposisi terbaik yang menghasilkan tempe dengan kadar yodium 0,26 mg/g; nilai sensoris hedonik rata-rata 5 (agak suka), serta 100 gram tempe tersebut memenuhi 17,47% angka kecukupan gizi (AKG) yodium orang dewasa per hari. Studi ini menunjukkan bahwa tempe tersebut dapat digolongkan sebagai pangan sumber yodium karena telah memenuhi 15% AKG orang dewasa.ABSTRACTTempeh is a highly nutritious food that is popular and accepted by diverse community groups, but has low iodine levels. Seaweed, with a high iodine content can be used as a fortification ingredient to increase the iodine levels of the foods. The addition of seaweed in tempeh is expected to be used as an alternative food source of iodine contents to overcome the IDD (Iodine Deficiency Disorders) problem. This study aimed to determine the effect of the addition of Gracilaria sp. seaweed flour to iodine levels in tempeh and to determine the characteristic of the product. The treatments were 5 (five) compositions of soybean and seaweed flour at ratio of (100:0), (97.5:2.5), (95:5), (92.5:7.5), and (90:10). The composition of soybean and seaweed flour at ratio of 92.5:7.5 was the best composition that produces tempeh with an iodine content of 0.26 mg/g; hedonic sensory test results on average 5 (somewhat like) and 100 g of tempeh met 17.47% of the daily iodine requirements for the adults. This study showed that tempeh can be considered as an alternative food source of iodine content as it contributed to 15% of adult’s daily nutritional adequacy.
Biodesulfurization of the mixture of dibenzothiophene and its alkylated derivatives by Sphingomonas subarctica T7b Ida Bagus Wayan Gunam; Teruo Sone; Kozo Asano
Indonesian Journal of Biotechnology Vol 26, No 3 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.62584

Abstract

Organosulfur compounds classified as dibenzothiophenes (DBTs) and their derivatives are contained in petroleum. When used as fuel, these substances release SOx emissions, thus contributing to air pollution, acid rain, and climate change. Therefore, it is necessary to reduce the content of these organic sulfur compounds in fuels and one way to achieve this is through bacterial desulfurization. This study reports the biodesulfurization process of a mixture of DBT, 4-hexyl DBT, 4,6-dibutyl DBT, and various organosulfur compounds in light gas oil (LGO). The experiment was conducted by treating 1 mL of aromatic organosulfur compounds with 100 mg/L in \textit{n}-tetradecane or 1 mL LGO with 5 mL mineral salts in sulfur-free medium, incubated at 27 °C for 5 days with shaking at 273 rpm. Gas chromatography analyses revealed that the growing Sphingomonas subarctica T7b cells desulfurized and converted 88.29% of DBT to 2-hydroxybiphenyl as a metabolite while a mixture of DBT and 4,6-dibutyl DBT was desulfurized at 86.40\% and 7.00%, respectively. Furthermore, the mixture of DBT, 4-hexyl DBT, and 4,6-dibutyl DBT had a desulfurization percentage of 84.40%, 41.00%, and 6.66%, respectively, after five days of incubation. The compounds were observed to desulfurize slightly better as single compounds compared to when mixed with other aromatic sulfur compounds.
PEMANFAATAN SAMPAH ORGANIK MENJADI PUPUK KOMPOS DENGAN BANTUAN MIKROORGANISME DI DESA SIBETAN KARANGASEM WAYAN GUNAM; I.B., L.P. WRASIATI; I MD. MAHAPUTRA WIJAYA; IW. ARNATA; I W.G. SEDANA YOGA
Buletin Udayana Mengabdi Vol 7 No 2 (2008): Volume 7 No.2 – September 2008
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (21.101 KB)

Abstract

ABSTRACT Sibetan village which located in Karangasem Regency eastern of Bali is well known for its zallaca farming, that produces high quality and original taste of zallaca fruits. Zallaca,s post harvest process in farmer’s and distributor’s level produces enough quantity of organic waste. This waste currently is become a pollutant to their neighborhood and their soil. Even tough the majority of the waste is organic, it still became a disturbance because of its large volume. Hence, a special method or treatment is needed to solve this problem. Composting this organic waste through the helps of micro-organisms is became the selected method because this process is able to decreases the volume of the organic waste, and also the fermented waste is useful as an organic fertilizer. The fermented waste add a value to the zallaca’s farmer in order to decrease the dependency to artificial fertilizer, and helps increase the income of the zallaca’s farmer with the decrease allocations of farming expenses to bought an artificial fertilizer.
Microbiological And Physicochemical Changes Of Green Coffee (Coffea Arabica) Fermentation In Kintamani, Bangli, Bali Sayi Hatiningsih; Nyoman Semadi Antara; Ida Bagus Wayan Gunam
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 5 No 2 (2018): Scientific Journal of Food Technology (September)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

The excellent quality of the fermentation process is closely related to microbes involved in the process fermentation. This study aimed to compare the microbiological changes and physicochemical quality of green coffee during 16 hours fermentation. Lactic acid bacteria and yeast were the predominant microorganisms found throughout the fermentation process. The total lactic acid bacteria and yeast reached the highest value of 9.31 log10 CFU/g and 9.10 log10 CFU/g respectively on the coffee bean and 9.30 log10 CFU/g and 9.10 log10 CFU/g respectively on liquid of fermented coffee bean. The quantity of microflora on the coffee bean and liquid of fermented coffee bean increased and reached its maximum value after twelve hours and decreased after exceed its peak, while fungi was no detected during fermentation. Sixteen hours fermentation significantly (P<0.05) influenced the bulk density, moisture content and bean weight/100 beans, bean amount/10 g and cause defect to green coffee such us “broken beans”, “brown beans” and “partly black beans” yet were not significantly affected (P>0.05) bean size and color of the green coffee. Throughout coffee fermentation, the acids on the green coffee increased from 5.33 ± 0.144 to 9.99 ± 0.144% and the caffeine on the green coffee decreased from 3.70 ± 0.017 to 1.66 ± 0.003%. Phenol content on the green coffee and its brews also decreased successively from 1381.58 ± 8.40 to 980.16 ± 10.07 mg GAEs/100 g and 922.25 ± 17.961 to 812.06 ± 12.660 mg GAEs/100 g. While for the antioxidants capacity, green coffee was more stable and its brews increased successively from 11564.28 ± 255.345 to 112 34.428 ± 174.377 mg GAEAC/L and 32326.24 ± 22.744 to 38658.62 ± 180.107 mg GAEAC/L. It can be concluded that coffee fermentation had more a positive effect on microbiological changes and physicochemical quality of green coffee.
Pengaruh Penambahan Kunyit (Curcuma domestica Val.) dan Lama Fermentasi Terhadap Karakteristik Mikrobiologis Urutan (Sosis Bali Terfermentasi) Nyoman Semadi Antara; IdaBagus Wayan Gunam; A.A.Made Dewi Anggreni
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 2 No 2 (2015)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

The aim of the research was to find out the effect of turmeric used in the urutan formulationon the microbiological characteristic of urutan. In production of urutan, two strains of lactic acidbacteria (LAB), Lactobacillus plantarum U201 and Pediococcus acidilactici U318, were used asmultiple starter culture. The results showed that the growth of LAB increased after 24 hfermentation. The addition of 0.5-1.0% turmeric could enhance the growth of LAB. The additionof 1-2% turmeric suppressed the growth of Enterobacteriaceae to undetected level after 48 hfermentation. The growth of Staphylococcus aureus tended to decrease during fermentation, andthese bacteria were not detected on urutan which used turmeric of 1-2% after 96 h fermentation.At the initial of fermentation the growth of micrococci increased, and the bacteria decreasedgradually from 48 h fermentation until the end of fermentation. In general, using turmeric of 1%in urutan formulation could enhance the growth of LAB and suppress the growth of undesirablebacteria, such as the group of Enterobacteriaceae and Staph. aureus.
KARAKTERISTIK WINE SALAK PADA BERBAGAI JENIS DAN KONSENTRASI BAHAN PENJERNIH Ida Bagus Wayan Gunam; Luh Putu Wrasiati; Deddy Setiadi Kadir Madjid
JURNAL GASTRONOMI INDONESIA Vol 4 No 1 (2016): Jurnal Gastronomi
Publisher : Pusat Penelitian & Pengabdian kepada Masyarakat Politeknik Pariwisata Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52352/jgi.v4i1.167

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh jenis dan konsentrasi bahan penjernih terhadap karakteristik wine salak, serta untuk menentukan jenis dan konsentrasi bahan penjernih yang dapat menghasilkan karakteristik wine salak yang terbaik. Rancangan yang dipergunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK) pola faktorialyang terdiri dari 2 faktor, faktor 1 adalah jenis bahan penjernih yang terdiri dari 3 level yaitu bentonit, kitosan dan gelatin. Faktor 2 adalah konsentrasi bahan penjernih yang terdiri dari 5 level yaitu 0,5 ppm; 1,0 ppm; 1,5 ppm; 2,0 ppm; 2, 5 ppm. Masing-masing perlakuan dikelompokkan menjadi 3 kelompok berdasarkan waktu penjernihannya, sehingga diperoleh 45 unit percobaan. Data yang diperoleh dianalisis dengan sidik ragam dan apabila terdapat pengaruh maka dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahan penjernih kitosan dengan konsentrasi 1,5 ppm diperoleh tingkat kejernihan yang terbaik 0,010 A, menghasilkan karakteristik wine salak dengan kadar alkohol 12,54%, pH4,2, dan total padatan terlarut 7,5 mg. L '. Aroma, citarasa dan penerimaan secara keseluruhan wine salak yang dijernihkan dengan kitosan 1,5 ppm disukai oleh panelis.
PENGARUH BERBAGAI MERK DRIED YEAST (Saccharomyces sp.) DAN pH AWAL FERMENTASI TERHADAP KARAKTERISTIK WINE SALAK Ida Bagus Wayan Gunam
Jurnal Teknologi & Industri Hasil Pertanian Vol 22, No 2 (2017): Jurnal Teknologi & Industri Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.558 KB) | DOI: 10.23960/jtihp.v22i2.63-72

Abstract

The purpose of this research were to find out the effect of different of dried yeast and initial pH of fermentation on the characteristic of salacca wine, and to determine the appropriate of kind of dried yeast and initial pH of fermentation to produce salacca wine with the best characteristic. This research used Randomized Block Design with factorial pattern. The first factor was the kind of dried yeast brand that consisted of 3 kinds namely Lalvin, Levure and Alcotec. The second factor consisted of 5 levels of initial pH, namely 3.5; 4; 4.5; 5; and 5.5. Each treatment was done 2 times, in order to obtain 30 trial units. The data obtained was analyzed using ANOVA followed by Duncans test. Interactions between treatments had very significant effect on the ethanol content, reduction sugar content, and pH, but had significant effect on acid total content. The best treatment was to use the Alcotec brand dried yeast with a treatment of initial pH as 4, with the following characteristics: ethanol content 12.40%, negative content of methanol, reduction sugar content 5.87%, acid total content 0.20%, pH 3.5 after the fermentation, the color was enough pure yellow, the flavor was strong enough, the taste was strong enough, and rather liked over all acceptance.
Pengaruh penambahan gum arab dan suhu penyimpanan terhadap kestabilan gel lidah buaya sebagai bahan edible coating [The effect of gum arabic addition and storage temperature on the stability of aloe vera gel as an edible coating] Ni Made Defy Janurianti; I Made Supartha Utama; Ida Bagus Wayan Gunam
Jurnal Teknologi & Industri Hasil Pertanian Vol 27, No 1 (2022): Jurnal Teknologi & Industri Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtihp.v27i1.53-62

Abstract

Aloe vera gel is a polysaccharide widely used as an edible coating because it contains glucomannan and lignin compounds that can inhibit respiration and transpiration in fruit. However, aloe vera gel has weakness such as easy to change its color, odor, and viscosity. Therefore, a stabilizer is needed, one of which is gum arabic. Storage temperature also affects the stability of aloe vera gel. The purpose of this study was to determine the concentration of gum arabic and the optimal storage temperature in stabilizing aloe vera gel as an edible coating. The study used a completely randomized design (CRD) with a factorial pattern. Parameters tested were viscosity, pH, color change, and sedimentation percentage. The results showed that the optimal concentration of gum arabic to produce stable aloe vera gel was 2% in cold storage (5 ± 1ºC), with stability up to 9 days of storage, on the condition that aloe vera gel has a pH of 4.58 ± 0.04, viscosity 40.67 ± 0.58 m.Pa.s, sedimentation percentage 8.13 ± 14.08% and color change (∆E) during storage 20, 09 ± 0.58. Aloe vera gel added with 2% arabic gum can be an edible coating on fruit or vegetables.
Co-Authors A.A.S. A. Sukmaningsih Ab Wahabb, Roswanira Abdul Wahab, Roswanira Adekilekun , Habeebulahi Ajibola Adekilekun Oyewusi, Habeebat Adekilekun, Habeebulahi Akinyede, Kolajo Ambar Kusumaningrum Amna Hartiati Anak Agung Made Dewi Anggreni Azis Akbar Hakim Bambang Admadi Harsojuwono Bimby Issassam Damuri, Nur Wahida Deddy Setiadi Kadir Madjid Dwi Ayu Kirani Paramita Dylla Hanggaeni Dyah Puspaningrum Ella Dewi Yusnia ENIEK KRISWIYANTI Erlangga, Muhammad Agung Fitra Ayu Sitanggang G.P. Ganda Putra Gde Agung Bagus Surya Adnyana Gede Bagus Suwarrizki G. P Hamonangan Sipayung Huda , Nurul Huyop, Fahrul I B. K. Widnyana Yoga I Dewa Gde Mayun Permana I Dewa Putu Rega Elyana I Gede Arya Sujana I Gede Krisna Putra Pratama I Gusti Ayu Lani Triani I Gusti Bagus Ananta Wijaya Putra I Gusti Ngurah Agung Paranatha I Kadek Adi Wijaya Putra I Ketut Satriawan I Komang Trikuti I M. Mahaputra Wijaya I Made Mahaputra Wijaya I Made Mahaputra Wijaya I Made Sugitha I MADE SUPARTHA UTAMA I Made Supartha Utama I Made Yoga Saputra I MADE YOGA SEMARA GUNA I Putu Hendra Prasetya I W W Aryanika I W. Wisma P. Putra I W.G. SEDANA YOGA I Wayan Arnata I Wayan Kasa I Wayan Suarta I Wayan Widia I Wayan Wisma Pradnyana Putra I.M.S. Wijaya IDA AYU ASTARINI Ida Bagus Gede Brahmantara IDA BAGUS N. SURYA DARMA Ida Bagus Wisnu Matra Atmaja Izzah Ismail, Norjihada Janurianti , Ni Made Defy Janurianti, Ni Made Defy Ketut Agus Ary Subakti KETUT BUDA KOMANG AYU NOCIANITRI Kozo Asano Luh Putu Wrasiati Lutfi Suhendra Made Dewi Anggreni, Anak Agung Made Ria Defiani Mahaputra Wijaya Mertayasa, I Made Michael Jordi Theofanny Mohd Rozdhi, Amira Azawani Mohd Shariff , Amir Husni Monalisa Nababan Monikasari, Ni Nyoman Trisa Muhammad Iqbal N.P.I. Widyantari Nebay Cronika Simbolon Ni Kadek Eni Juniantari Ni Made Ratih Despianti Ni Made Wartini Ni Putu Puspadi Aristyanti Ni Putu Suwariani Ni Wayan Wisaniyasa Noval Wahyu Adi Nursatria Purba Nurul Octavia Wasis NYOMAN SEMADI ANTARA Odilia Keron Oladimeji Tugbobo, Samuel Oladipo, Oluwatosin Oyewusi, Habeebat Adekilekun Pande Ketut Diah Kencana Pande Komang Suparyana Prameshwari, Jamas Purnami, Ni Gusti Ayu Kade Sintya Ari Putu Agus Nadiarta Putu Setia Budi Rajindran, Nanthini Rendy Sinaga Rike Pratiwi Sadyasmara, Cokorda Anom Bayu Sandhi Wipradnyadewi, Putu Ari Sayi Hatiningsih Shenni Maulina Sujana, I Gede Arya Suputra, I Gede Maha Adijaya Tantri, Ida Ayu Pridari Teguh Pribadi Girsang Teruo Sone Thobie E. B Kaban Tirmizhi Abubakar, Munkaila WAYAN REDI ARYANTA Wijaya, I Made Mahaputra Wito Setioko Yohanes Setiyo Yohannes Eko Putra Simanullang Yudisthira Dharma Bhusana Dasa Zainul Arifin